Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | maintains catalytic activity of PMH | Streptomyces morookaense |
Application | Comment | Organism |
---|---|---|
additional information | amino acid sequence of PMH displays high similarity to that of the Streptomyces acyl-peptide hydrolase. PMH is an aminopeptidase carrying a putative catalytic triad, Ser511-Asp593-His625, with broad substrate specificity | Streptomyces morookaense |
Cloned (Comment) | Organism |
---|---|
pTrc-PMH overexpressed in Escherichia coli strain JM105 | Streptomyces morookaense |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
Pro-beta-naphthylamide | - |
Streptomyces morookaense | |
0.028 | - |
Phe-beta-naphthylamide | - |
Streptomyces morookaense | |
0.067 | - |
Tyr-beta-naphthylamide | - |
Streptomyces morookaense | |
0.12 | - |
Leu-beta-naphthylamide | - |
Streptomyces morookaense | |
0.39 | - |
Ala-beta-naphthylamide | - |
Streptomyces morookaense | |
1.91 | - |
puromycin | - |
Streptomyces morookaense |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces morookaense | - |
strain JCM4673 | - |
Streptomyces morookaense JCM 4673 | - |
strain JCM4673 | - |
Purification (Comment) | Organism |
---|---|
by gel filtration, to homogeneity | Streptomyces morookaense |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.124 | - |
recombinant enzyme, after the induction with IPTG, at 8 h of culture in LB medium | Streptomyces morookaense |
0.172 | - |
recombinant enzyme, after the induction with IPTG, at 18 h of culture in TB medium | Streptomyces morookaense |
13 | - |
with Tyr-Leu as substrate | Streptomyces morookaense |
19 | - |
with Tyr-Phe as substrate | Streptomyces morookaense |
23 | - |
with Ala-p-nitroanilide as substrate | Streptomyces morookaense |
154 | - |
with Phe-p-nitroanilide as substrate | Streptomyces morookaense |
186 | - |
with Ala-beta-naphthylamide as substrate | Streptomyces morookaense |
Storage Stability | Organism |
---|---|
-30°C, 20% glycerol | Streptomyces morookaense |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ala-beta-naphthylamide + H2O | - |
Streptomyces morookaense | Ala + 2-naphthylamine | - |
? | |
Ala-beta-naphthylamide + H2O | - |
Streptomyces morookaense JCM 4673 | Ala + 2-naphthylamine | - |
? | |
Ala-p-nitroanilide + H2O | prefered substrate for PMH | Streptomyces morookaense | Ala + p-nitroaniline | - |
? | |
Ala-p-nitroanilide + H2O | prefered substrate for PMH | Streptomyces morookaense JCM 4673 | Ala + p-nitroaniline | - |
? | |
Leu-beta-naphthylamide + H2O | - |
Streptomyces morookaense | Leu + 2-naphthylamine | - |
? | |
Leu-beta-naphthylamide + H2O | - |
Streptomyces morookaense JCM 4673 | Leu + 2-naphthylamine | - |
? | |
Phe-beta-naphthylamide + H2O | - |
Streptomyces morookaense | Phe + 2-naphthylamine | - |
? | |
Phe-p-nitroanilide + H2O | prefered substrate for PMH | Streptomyces morookaense | Phe + p-nitroaniline | - |
? | |
Pro-beta-naphthylamide + H2O | - |
Streptomyces morookaense | Pro + 2-naphthylamine | - |
? | |
puromycin + H2O | - |
Streptomyces morookaense | ? | - |
? | |
Tyr-beta-naphthylamide + H2O | - |
Streptomyces morookaense | Tyr + 2-naphthylamine | - |
? | |
Tyr-Leu + H2O | - |
Streptomyces morookaense | Tyr + Leu | - |
? | |
Tyr-Leu + H2O | - |
Streptomyces morookaense JCM 4673 | Tyr + Leu | - |
? | |
Tyr-Phe + H2O | - |
Streptomyces morookaense | Tyr + Phe | - |
? | |
Tyr-Phe + H2O | - |
Streptomyces morookaense JCM 4673 | Tyr + Phe | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PM hydrolase | - |
Streptomyces morookaense |
PMH | - |
Streptomyces morookaense |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.98 | - |
puromycin | - |
Streptomyces morookaense | |
1.61 | - |
Pro-beta-naphthylamide | - |
Streptomyces morookaense | |
1.69 | - |
Phe-beta-naphthylamide | - |
Streptomyces morookaense | |
1.85 | - |
Tyr-beta-naphthylamide | - |
Streptomyces morookaense | |
4.07 | - |
Leu-beta-naphthylamide | - |
Streptomyces morookaense | |
8.79 | - |
Ala-beta-naphthylamide | - |
Streptomyces morookaense |