Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli as a functional enzyme | Cucumis sativus |
expression in Escherichia coli as a functional enzyme | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 1 mM, complete inhibition of recombinant enzyme | Arabidopsis thaliana | |
Cu2+ | 1 mM, complete inhibition of recombinant enzyme | Cucumis sativus | |
diisopropyl fluorophosphate | 1 mM, complete inactivation | Arabidopsis thaliana | |
diisopropyl fluorophosphate | 1 mM, complete inactivation | Cucumis sativus | |
NEM | 1 mM | Arabidopsis thaliana | |
NEM | 1 mM | Cucumis sativus | |
PCMB | 0.1 mM | Arabidopsis thaliana | |
PCMB | 0.1 mM | Cucumis sativus | |
PMSF | 1 mM, 50% inhibition | Arabidopsis thaliana | |
PMSF | 1 mM, 50% inhibition | Cucumis sativus | |
Zn2+ | 1 mM, complete inhibition of recombinant enzyme | Arabidopsis thaliana | |
Zn2+ | 1 mM, complete inhibition of recombinant enzyme | Cucumis sativus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
N-acetyl-Ala-p-nitroanilide | pH 7.0, 37°C | Arabidopsis thaliana | |
4.6 | - |
N-acetyl-Ala-p-nitroanilide | pH 7.0, 37°C | Cucumis sativus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast stroma | - |
Cucumis sativus | 9570 | - |
chloroplast stroma | - |
Arabidopsis thaliana | 9570 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
82000 | - |
4 * 82000, SDS-PAGE | Cucumis sativus |
82000 | - |
4 * 82000, SDS-PAGE | Arabidopsis thaliana |
350000 | - |
gel filtration | Cucumis sativus |
350000 | - |
gel filtration | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Cucumis sativus | the enzyme might be involved in not only catalysis of the N-terminal hydrolysis of Nalpha-acylpeptides but also the elimination of glycated proteins | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q84LM4 | - |
- |
Cucumis sativus | - |
L. suyo | - |
Purification (Comment) | Organism |
---|---|
- |
Cucumis sativus |
- |
Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Cucumis sativus | - |
shoot | - |
Cucumis sativus | - |
shoot | - |
Arabidopsis thaliana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.9 | - |
- |
Cucumis sativus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-Ala-Ala + H2O | - |
Cucumis sativus | acetyl-Ala + Ala | - |
? | |
acetyl-Ala-Ala + H2O | - |
Arabidopsis thaliana | acetyl-Ala + Ala | - |
? | |
Acetyl-Ala-Ala-Ala + H2O | native enzyme shows 147% of the activity compared to acetyl-Ala-Ala as substrate | Cucumis sativus | Acetyl-Ala + Ala-Ala | - |
? | |
Acetyl-Ala-Ala-Ala + H2O | native enzyme shows 81% of the activity compared to acetyl-Ala-Ala as substrate | Arabidopsis thaliana | Acetyl-Ala + Ala-Ala | - |
? | |
acetyl-Ala-Ala-Ala-Ala + H2O | native enzyme shows 126% of the activity compared to acetyl-Ala-Ala as substrate | Arabidopsis thaliana | acetyl-Ala + Ala-Ala-Ala | - |
? | |
acetyl-Ala-Ala-Ala-Ala + H2O | native enzyme shows 85.6% of the activity compared to acetyl-Ala-Ala as substrate | Cucumis sativus | acetyl-Ala + Ala-Ala-Ala | - |
? | |
acetyl-Ala-Met + H2O | native enzyme shows 70.4% of the activity compared to acetyl-Ala-Ala as substrate | Cucumis sativus | acetyl-Ala + Met | - |
? | |
acetyl-Gly-Gly + H2O | native enzyme shows 37% of the activity compared to acetyl-Ala-Ala as substrate | Cucumis sativus | acetyl-Gly + Gly | - |
? | |
acetyl-Gly-Gly + H2O | native enzyme shows 4% of the activity compared to acetyl-Ala-Ala as substrate | Arabidopsis thaliana | acetyl-Gly + Gly | - |
? | |
acetyl-Gly-Leu + H2O | native enzyme shows 30.3% of the activity compared to acetyl-Ala-Ala as substrate | Cucumis sativus | acetyl-Gly + Leu | - |
? | |
acetyl-Met-Ala + H2O | native enzyme shows 4% of the activity compared to acetyl-Ala-Ala as substrate | Cucumis sativus | acetyl-Met + Ala | - |
? | |
acetyl-Met-Ala + H2O | native enzyme shows 45% of the activity compared to acetyl-Ala-Ala as substrate | Arabidopsis thaliana | acetyl-Met + Ala | - |
? | |
acetyl-Met-Asn + H2O | native enzyme shows 34.1% of the activity compared to acetyl-Ala-Ala as substrate | Cucumis sativus | acetyl-Met + Asn | - |
? | |
acetyl-Met-Glu + H2O | native enzyme shows 4.3% of the activity compared to acetyl-Ala-Ala as substrate | Cucumis sativus | acetyl-Met + Glu | - |
? | |
acetyl-Met-Glu + H2O | native enzyme shows 6% of the activity compared to acetyl-Ala-Ala as substrate | Arabidopsis thaliana | acetyl-Met + Glu | - |
? | |
acetyl-Met-Phe + H2O | native enzyme shows 5% of the activity compared to acetyl-Ala-Ala as substrate | Arabidopsis thaliana | acetyl-Met + Phe | - |
? | |
butyryl-Ala-Ala-Ala + H2O | native enzyme shows 33.6% of the activity compared to acetyl-Ala-Ala as substrate | Cucumis sativus | butyryl-Ala + Ala-Ala | - |
? | |
butyryl-Ala-Ala-Ala + H2O | native enzyme shows 77% of the activity compared to acetyl-Ala-Ala as substrate | Arabidopsis thaliana | butyryl-Ala + Ala-Ala | - |
? | |
formyl-Ala-Ala-Ala + H2O | native enzyme shows 62.4% of the activity compared to acetyl-Ala-Ala as substrate | Cucumis sativus | formyl-Ala + Ala-Ala | - |
? | |
formyl-Ala-Ala-Ala + H2O | native enzyme shows 81% of the activity compared to acetyl-Ala-Ala as substrate | Arabidopsis thaliana | formyl-Ala + Ala-Ala | - |
? | |
formyl-Gly-Val + H2O | native enzyme shows 30.3% of the activity compared to acetyl-Ala-Ala as substrate | Cucumis sativus | formyl-Gly + Val | - |
? | |
glycated ribulose-1,5-diphosphate carboxylase/oxygenase protein + H2O | no degradation of the native protein | Cucumis sativus | ? | - |
? | |
additional information | the enzyme might be involved in not only catalysis of the N-terminal hydrolysis of Nalpha-acylpeptides but also the elimination of glycated proteins | Cucumis sativus | ? | - |
? | |
N-acetyl-Ala-p-nitroanilide + H2O | substrates in the order of catalytic efficiency: N-acetyl-Ala-p-nitranilide, N-acetyl-Gly-p-nitranilide, N-acetyl-Met-p-nitranilide | Cucumis sativus | N-acetyl-Ala + p-nitroaniline | - |
? | |
N-acetyl-Ala-p-nitroanilide + H2O | substrates in the order of catalytic efficiency: N-acetyl-Ala-p-nitranilide, N-acetyl-Met-p-nitranilide, N-acetyl-Gly-p-nitranilide | Arabidopsis thaliana | N-acetyl-Ala + p-nitroaniline | - |
? | |
N-acetyl-Gly-p-nitroanilide + H2O | substrates in the order of catalytic efficiency: N-acetyl-Ala-p-nitranilide, N-acetyl-Gly-p-nitranilide, N-acetyl-Met-p-nitranilide | Cucumis sativus | N-acetyl-Gly + p-nitroaniline | - |
? | |
N-acetyl-Gly-p-nitroanilide + H2O | substrates in the order of catalytic efficiency: N-acetyl-Ala-p-nitranilide, N-acetyl-Met-p-nitranilide, N-acetyl-Gly-p-nitranilide | Arabidopsis thaliana | N-acetyl-Gly + p-nitroaniline | - |
? | |
N-acetyl-Met-p-nitroanilide + H2O | substrates in the order of catalytic efficiency: N-acetyl-Ala-p-nitranilide, N-acetyl-Gly-p-nitranilide, N-acetyl-Met-p-nitranilide | Cucumis sativus | N-acetyl-Met + p-nitroaniline | - |
? | |
N-acetyl-Met-p-nitroanilide + H2O | substrates in the order of catalytic efficiency: N-acetyl-Ala-p-nitranilide, N-acetyl-Met-p-nitranilide, N-acetyl-Gly-p-nitranilide | Arabidopsis thaliana | N-acetyl-Met + p-nitroaniline | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 82000, SDS-PAGE | Cucumis sativus |
tetramer | 4 * 82000, SDS-PAGE | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
AtAARE | - |
Arabidopsis thaliana |
cAARE | - |
Cucumis sativus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Cucumis sativus |
37 | - |
- |
Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Cucumis sativus |
7 | - |
- |
Arabidopsis thaliana |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Cucumis sativus | isoelectric focusing | - |
5.5 |