Application | Comment | Organism |
---|---|---|
diagnostics | Cathepsin X is an inflammatory marker | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression of the proenzyme in Pichia pastoris | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
bradykinin + H2O | Homo sapiens | the peptide is converted from a bradykinin B2 receptor ligand to a bradykinin B1 receptor specific ligand | ? | - |
? | |
kallidin + H2O | Homo sapiens | the peptide is converted from a bradykinin B2 receptor ligand to a bradykinin B1 receptor specific ligand | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | processing of the recombinant proenzyme by cathepsin L | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Pichia pastoris by cation exchange chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
bradykinin + H2O | i.e. Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg, a mainly B2 receptor | Homo sapiens | Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe + Arg | an exclusive B1 receptor | ? | |
bradykinin + H2O | the peptide is converted from a bradykinin B2 receptor ligand to a bradykinin B1 receptor specific ligand | Homo sapiens | ? | - |
? | |
kallidin + H2O | the peptide is converted from a bradykinin B2 receptor ligand to a bradykinin B1 receptor specific ligand | Homo sapiens | ? | - |
? | |
kallidin + H2O | i.e. Lys-Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg, a mainly B2 receptor | Homo sapiens | Lys-Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe + Arg | an exclusive B1 receptor | ? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | cathepsin X generates peptide receptor agonists and acts as a type I kininase. It is a cysteine-type carboxypeptidase and able to modulate the kallikreinkinin system through carboxyterminal processing of the small peptide hormones bradykinin and kallidin. Cathepsin X as an alternative possible link between the kallikreinkinin system and the reninangiotensin system in that it not only cleaves kinins C-terminally, but also converts angiotensin I to angiotensin II, reaction of EC 3.6.15.1 | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.17 | - |
bradykinin | pH 5.5 | Homo sapiens | |
5.75 | - |
kallidin | pH 5.5 | Homo sapiens |