Literature summary for 3.4.18.1 extracted from

Ahn, S.J.; Kim, N.Y.; Jeon, S.J.; Sung, J.H.; Je, J.E.; Seo, J.S.; Kim, M.S.; Kim, J.K.; Chung, J.K.; Lee, H.H.
Molecular cloning, tissue distribution and enzymatic characterization of cathepsin X from olive flounder (Paralichthys olivaceus) (2008), Comp. Biochem. Physiol. B, 151, 203-212.

Search for more literature for 3.4.18.1

Cloned(Commentary)

Cloned (Comment)	Organism
cDNA encoding for the proenzyme of PoCtX (proPoCtX) is expressed in Escherichia coli as a 57 kDa fusion protein with glutathione S-transferase	Paralichthys olivaceus

Inhibitors

Inhibitors	Comment	Organism	Structure
1,10-phenanthroline	0.1 mM, proPoCtX: 51.3% inhibition, native PoCtX: 56.6% inhibition	Paralichthys olivaceus
antipain	0.1 mM, proPoCtX: 98.6% inhibition, native PoCtX: 97.6% inhibition	Paralichthys olivaceus
Aprotinin	0.1 mM, proPoCtX: 31.9% inhibition, native PoCtX: 51.3% inhibition	Paralichthys olivaceus
chymostatin	0.1 mM, proPoCtX: 35.4% inhibition, native PoCtX: 96.9% inhibition	Paralichthys olivaceus
E-64	0.1 mM, proPoCtX: 25.6% inhibition, native PoCtX: 98.6% inhibition	Paralichthys olivaceus
EDTA	0.1 mM, proPoCtX: 5.1% inhibition, native PoCtX: 14.2% inhibition	Paralichthys olivaceus
EGTA	0.1 mM, proPoCtX: 2.2% inhibition, native PoCtX: 25.6% inhibition	Paralichthys olivaceus
leupeptin	0.1 mM, proPoCtX: 99.1% inhibition, native PoCtX: 95.6% inhibition	Paralichthys olivaceus
additional information	recombinant proPoCtX is inhibited by antipain and leupeptin	Paralichthys olivaceus
NEM	0.1 mM, proPoCtX: 44.6% inhibition, native PoCtX: 67.0% inhibition	Paralichthys olivaceus
pepstatin A	0.1 mM, proPoCtX: 0% inhibition, native PoCtX: 36.0% inhibition	Paralichthys olivaceus
PMSF	0.1 mM, proPoCtX: 42.2% inhibition, native PoCtX: 37.2% inhibition	Paralichthys olivaceus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Paralichthys olivaceus	5829	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
33200	-	native PoCtX protein	Paralichthys olivaceus

Organism

Organism	UniProt	Comment	Textmining
Paralichthys olivaceus	Q58HF4	-	-

Purification (Commentary)

Purification (Comment)	Organism
purified 9.48fold via a one-step purification process using a DEAE-Sephagel HPLC column	Paralichthys olivaceus

Source Tissue

Source Tissue	Comment	Organism	Textmining
alimentary canal	-	Paralichthys olivaceus	-
brain	-	Paralichthys olivaceus	-
eye	-	Paralichthys olivaceus	-
gill	-	Paralichthys olivaceus	-
heart	-	Paralichthys olivaceus	-
intestine	-	Paralichthys olivaceus	-
kidney	-	Paralichthys olivaceus	-
liver	-	Paralichthys olivaceus	-
muscle	-	Paralichthys olivaceus	-
pyloric cecum	-	Paralichthys olivaceus	-
spleen	-	Paralichthys olivaceus	-
stomach	-	Paralichthys olivaceus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
364.5	-	cytosol extract	Paralichthys olivaceus
3457	-	DEAE-Sephagel, 9.48fold purification	Paralichthys olivaceus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Z-Arg-Arg-7-amido-4-methylcoumarin + H2O	-	Paralichthys olivaceus	?	-	?
Z-Phe-Arg-7-amido-4-methylcoumarin + H2O	-	Paralichthys olivaceus	?	-	?

Synonyms

Synonyms	Comment	Organism
cathepsin X	-	Paralichthys olivaceus
PoCtX	-	Paralichthys olivaceus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Paralichthys olivaceus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5	-	recombinant proPoCtX	Paralichthys olivaceus
6	-	native PoCtX protein	Paralichthys olivaceus

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Release 2023.1 (January 2023)