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Literature summary for 3.4.18.1 extracted from

  • Klemencic, I.; Carmona, A.K.; Cezari, M.H.; Juliano, M.A.; Juliano, L.; Guncar, G.; Turk, D.; Krizaj, I.; Turk, V.; Turk, B.
    Biochemical characterization of human cathepsin X revealed that the enzyme is an exopeptidase, acting as carboxymonopeptidase or carboxydipeptidase (2000), Eur. J. Biochem., 267, 5404-5412.
    View publication on PubMed
Search for more literature for 3.4.18.1

Inhibitors

Inhibitors Comment Organism Structure
CA-074
-
 Homo sapiens
chicken cystatin
-
 Homo sapiens
cystatin C
-
 Homo sapiens
GFG-semicarbazone
-
 Homo sapiens
Stefin A
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
 (2,4-dinitrophenyl)-GFFW pH 5.1, 37°C Homo sapiens
0.0018
-
 (2,4-dinitrophenyl)-GFFGW pH 5.1, 37°C Homo sapiens
0.0024
-
 (2,4-dinitrophenyl)-GFRFW pH 5.1, 37°C Homo sapiens
0.0036
-
 (2,4-dinitrophenyl)-GFFRW pH 5.1, 37°C Homo sapiens
0.0037
-
 ortho-aminobenzoyl-Phe-Arg-(2,3-diaminopropionyl)-(2,4-dinitrophenyl)-Pro pH 5.1, 37°C Homo sapiens
0.0042
-
 (2,4-dinitrophenyl)-GFRW pH 5.1, 37°C Homo sapiens
0.0046
-
 (2,4-dinitrophenyl)-GRFFW pH 5.1, 37°C Homo sapiens
0.0083
-
 ortho-aminobenzoyl-Arg-Arg-(2,3-diaminopropionyl)-(2,4-dinitrophenyl)-Pro + pH 5.1, 37°C Homo sapiens
0.0105
-
 ortho-aminobenzoyl-Lys-Arg-(2,3-diaminopropionyl)-(2,4-dinitrophenyl)-Pro pH 5.1, 37°C Homo sapiens
0.063
-
 CBZ-FR-7-amido-4-methylcoumarin pH 5.1, 37°C Homo sapiens
0.13
-
 CBZ-RR-7-amido-4-methylcoumarin pH 5.1, 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein mature cathepsin X has at least one of the two potential glycosylation sites glycosylated Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2,4-dinitrophenyl)-GFFGW + H2O
-
 Homo sapiens (2,4-dinitrophenyl)-GFFG + L-Trp
-
 ?
(2,4-dinitrophenyl)-GFFRW + H2O
-
 Homo sapiens (2,4-dinitrophenyl)-GFFR + L-Trp
-
 ?
(2,4-dinitrophenyl)-GFFW + H2O
-
 Homo sapiens (2,4-dinitrophenyl)-GFF + L-Trp
-
 ?
(2,4-dinitrophenyl)-GFRFW + H2O
-
 Homo sapiens (2,4-dinitrophenyl)-GFR + L-Phe-L-Trp
-
 ?
(2,4-dinitrophenyl)-GFRW + H2O
-
 Homo sapiens (2,4-dinitrophenyl)-GFR + L-Trp
-
 ?
(2,4-dinitrophenyl)-GRFFW + H2O
-
 Homo sapiens (2,4-dinitrophenyl)-GRFF + L-Trp
-
 ?
(2-aminobenzoyl)-FFF + H2O
-
 Homo sapiens (2-aminobenzoyl)-FF + L-Phe
-
 ?
(2-aminobenzoyl)-FFFA + H2O
-
 Homo sapiens (2-aminobenzoyl)-FFF + L-Ala
-
 ?
(2-aminobenzoyl)-FFFP + H2O
-
 Homo sapiens (2-aminobenzoyl)-FFF + Pro
-
 ?
(2-aminobenzoyl)-FFFR + H2O
-
 Homo sapiens (2-aminobenzoyl)-FFF + L-Arg
-
 ?
(2-aminobenzoyl)-FFFR-NH2 + H2O
-
 Homo sapiens (2-aminobenzoyl)-FFF + Arg-amide
-
 ?
(2-aminobenzoyl)-FFFW + H2O
-
 Homo sapiens (2-aminobenzoyl)-FFF + L-Trp
-
 ?
(2-aminobenzoyl)-FFGW + H2O
-
 Homo sapiens (2-aminobenzoyl)-FFG + L-Trp
-
 ?
(2-aminobenzoyl)-FFRW + H2O
-
 Homo sapiens (2-aminobenzoyl)-FFR + L-Trp
-
 ?
(2-aminobenzoyl)-FFRW-NH2 + H2O
-
 Homo sapiens (2-aminobenzoyl)-FFR + L-Trp-amide
-
 ?
(2-aminobenzoyl)-FRFW-NH2 + H2O
-
 Homo sapiens (2-aminobenzoyl)-FR + Phe-Trp-amide
-
 ?
2-aminobenzoyl-Arg-Arg-(2,3-diaminopropionyl)-(2,4-dinitrophenyl)-Pro + H2O
-
 Homo sapiens ?
-
 ?
2-aminobenzoyl-Leu-Arg-(2,3-diaminopropionyl)-(2,4-dinitrophenyl)-Pro + H2O
-
 Homo sapiens ?
-
 ?
2-aminobenzoyl-Phe-Arg-(2,3-diaminopropionyl)-(2,4-dinitrophenyl)-Pro + H2O
-
 Homo sapiens ?
-
 ?
3-aminobenzoyl-FR-(2,3-diaminopropionyl)-(2,4-dinitrophenyl)-P + H2O
-
 Homo sapiens 3-aminobenzoyl-FR + (2,3-diaminopropionyl)-(2,4-dinitrophenyl)-P
-
 ?
3-aminobenzoyl-LR-(2,3-diaminopropionyl)-(2,4-dinitrophenyl)-P + H2O
-
 Homo sapiens 3-aminobenzoyl-LR + (2,3-diaminopropionyl)-(2,4-dinitrophenyl)-P
-
 ?
3-aminobenzoyl-RR-(2,3-diaminopropionyl)-(2,4-dinitrophenyl)-P + H2O
-
 Homo sapiens 3-aminobenzoyl-RR + (2,3-diaminopropionyl)-(2,4-dinitrophenyl)-P
-
 ?
benzyloxycarbonyl-FR-4-methylcoumaryl-7-amide + H2O
-
 Homo sapiens benzyloxycarbonyl-FR + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-RR-4-methylcoumaryl-7-amide + H2O
-
 Homo sapiens benzyloxycarbonyl-RR + 7-amino-4-methylcoumarin
-
 ?
additional information the enzyme normally acts as a carboxymonopeptidase. The preference for Arg in the P1 position is so strong that cathepsin X cleaves substrates with Arg in antepenultimate position, acting also as a carboxypeptidase. A large hydrophobic residue such as Trp is preferred in the P1' position, although the enzyme cleaves all P1' residues investigated (Trp, Phe, Ala, Arg, Pro). The enzyme also cleaves the substrates with amide-blocked C-terminal carboxyl group with rates similar to that of the unblocked substrates Homo sapiens ?
-
 ?
ortho-aminobenzoyl-Arg-Arg-(2,3-diaminopropionyl)-(2,4-dinitrophenyl)-Pro + H2O
-
 Homo sapiens ?
-
 ?
ortho-aminobenzoyl-Lys-Arg-(2,3-diaminopropionyl)-(2,4-dinitrophenyl)-Pro + H2O
-
 Homo sapiens ?
-
 ?
ortho-aminobenzoyl-Phe-Arg-(2,3-diaminopropionyl)-(2,4-dinitrophenyl)-Pro + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
cathepsin Z
-
 Homo sapiens
cysteine-type carboxypeptidase
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.007
-
 (2,4-dinitrophenyl)-GFFGW pH 5.1, 37°C Homo sapiens
0.02
-
 2-aminobenzoyl-Leu-Arg-(2,3-diaminopropionyl)-(2,4-dinitrophenyl)-Pro + pH 5.1, 37°C Homo sapiens
0.03
-
 (2,4-dinitrophenyl)-GFRFW pH 5.1, 37°C Homo sapiens
0.06
-
 2-aminobenzoyl-Arg-Arg-(2,3-diaminopropionyl)-(2,4-dinitrophenyl)-Pro + pH 5.1, 37°C Homo sapiens
0.07
-
 (2,4-dinitrophenyl)-GFFW pH 5.1, 37°C Homo sapiens
0.07
-
 2-aminobenzoyl-Phe-Arg-(2,3-diaminopropionyl)-(2,4-dinitrophenyl)-Pro + pH 5.1, 37°C Homo sapiens
0.22
-
 benzyloxycarbony-FR-4-methylcoumaryl-7-amide pH 5.1, 37°C Homo sapiens
0.23
-
 (2,4-dinitrophenyl)-GRFFW pH 5.1, 37°C Homo sapiens
0.3
-
 (2,4-dinitrophenyl)-GFFRW pH 5.1, 37°C Homo sapiens
0.63
-
 benzyloxycarbony-RR-4-methylcoumaryl-7-amide pH 5.1, 37°C Homo sapiens
0.7
-
 (2,4-dinitrophenyl)-GFRW pH 5.1, 37°C Homo sapiens
6.08
-
 benzyloxycarbony-RR-4-methylcoumaryl-7-amide pH 5.1, 37°C Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 reaction with 3-aminobenzoyl-FR-(2,3-diaminopropionyl)-(2,4-dinitrophenyl)-P Homo sapiens
5 5.5 reaction with benzyloxycarbonyl-RR-4-methylcoumaryl-7-amide Homo sapiens
5 6 reaction with (2,4-dinitrophenyl)-GFFW Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
3.5 5.5 pH 3.5: about 50% of maximal activity, pH 5.5: about 50% of maximal activity, reaction with 3-aminobenzoyl-FR-(2,3-diaminopropionyl)-(2,4-dinitrophenyl)-P Homo sapiens
4 6.5 pH 4.0: about 45% of maximal activity, reaction with benzyloxycarbonyl-RR-methylcoumarinamide Homo sapiens
4.5 6.5 pH 4.5: about 45% of maximal activity, pH 6.5: about 10% of maximal activity, reaction with (2,4-dinitrophenyl)-GFFW Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000017
-
 Stefin A pH 5.5, 25°C Homo sapiens
0.000012
-
 cystatin C pH 5.5, 25°C Homo sapiens
0.000015
-
 chicken cystatin pH 5.5, 25°C Homo sapiens
0.0166
-
 GFG-semicarbazone pH 5.5, 25°C Homo sapiens

pI Value

Organism Comment pI Value Maximum pI Value
Homo sapiens isoelectric focusing
-
 5.1