General Stability | Organism |
---|---|
10% glycerol stabilizes | Saccharolobus solfataricus |
13% remaining activity in 0.1% SDS at 70°C after 180 min, stable at 40°C | Saccharolobus solfataricus |
40% remaining activity in 8 M urea after 55 min at 40°C | Saccharolobus solfataricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | complete inhibition, can be reversed by Zn2+ or to a lesser extent by Co2+ | Saccharolobus solfataricus | |
o-phenanthroline | - |
Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.061 | - |
benzyloxycarbonyl-L-Arg | pH 6.5, 60°C | Saccharolobus solfataricus | |
0.17 | - |
benzoyl-glycyl-arginine | pH 6.5, 60°C | Saccharolobus solfataricus | |
0.22 | - |
Benzyloxycarbonyl-Asp | pH 6.5, 60°C | Saccharolobus solfataricus | |
0.23 | - |
benzoyl-glycyl-L-lysine | pH 6.5, 60°C | Saccharolobus solfataricus | |
0.31 | - |
benzyloxycarbonyl-Phe | pH 6.5, 60°C | Saccharolobus solfataricus | |
0.69 | - |
benzyloxycarbonyl-Gly-Gly-Phe | pH 6.5, 60°C | Saccharolobus solfataricus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Saccharolobus solfataricus | 5622 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | can partially substitute for Zn2+, activates | Saccharolobus solfataricus | |
additional information | Ni2+, Mn2+, Ca2+, and Mg2+ are ineffective | Saccharolobus solfataricus | |
Zn2+ | metalloprotease, dependent on | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
4 * 42000, SDS-PAGE | Saccharolobus solfataricus |
170000 | - |
gel filtration | Saccharolobus solfataricus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
peptide + H2O | Saccharolobus solfataricus | - |
? | - |
? | |
peptide + H2O | Saccharolobus solfataricus P2 | - |
? | - |
? | |
protein + H2O | Saccharolobus solfataricus | - |
peptides | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
acetonitril | 93% remaining activity in 50% acetonitril at 40°C after 101 min, inactivation at 70°C | Saccharolobus solfataricus |
Ethanol | 90% remaining activity in pure ethanol at 40°C after 98 min, inactivation over this time period at 70°C, 2% remaining activity in 50% ethanol at 70°C | Saccharolobus solfataricus |
Methanol | 56% remaining activity in pure methanol at 40°C after 98 min, inactivation over this time period at 70°C, 11% remaining activity in 50% methanol at 70°C | Saccharolobus solfataricus |
tetrahydrofuran | 19% remaining activity in 50% tetrahydrofuran after 42 min at 40°C, nactivation at 70°C | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | P80092 | strain MT-4, ATCC 49155 | - |
Saccharolobus solfataricus P2 | P80092 | strain MT-4, ATCC 49155 | - |
Purification (Comment) | Organism |
---|---|
to electrophoretic homogeneity, 76fold | Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
substrate specificity, esterase activity | Saccharolobus solfataricus |
4.1 | - |
purified enzyme, substrate benzyloxycarbonyl-Phe | Saccharolobus solfataricus |
8.6 | - |
purified enzyme, substrate benzyloxycarbonyl-Asp | Saccharolobus solfataricus |
16.3 | - |
purified enzyme, substrate benzyloxycarbonyl-Arg | Saccharolobus solfataricus |
21.9 | - |
purified enzyme, substrate benzoyl-glycyl-arginine | Saccharolobus solfataricus |
27.2 | - |
purified enzyme, substrate benzoyl-glycyl-lysine | Saccharolobus solfataricus |
38.7 | - |
purified enzyme, substrate benzyloxycarbonyl-Gly-Gly-Phe | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzoyl-glycyl-arginine + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
benzoyl-glycyl-L-lysine + H2O | - |
Saccharolobus solfataricus | benzoyl-glycine + L-lysine | - |
? | |
benzoyl-glycyl-lysine + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
benzyloxycarbonyl-Ala-Ser-methyl ester + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
benzyloxycarbonyl-Ala-Val-methyl ester + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
benzyloxycarbonyl-Arg + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
benzyloxycarbonyl-Asp + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
benzyloxycarbonyl-Glu-methyl ester + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
benzyloxycarbonyl-Gly-Gly-Phe + H2O | best substrate | Saccharolobus solfataricus | ? | - |
? | |
benzyloxycarbonyl-Gly-Gly-Phe + H2O | best substrate | Saccharolobus solfataricus P2 | ? | - |
? | |
benzyloxycarbonyl-L-Arg + H2O | - |
Saccharolobus solfataricus | benzyloxycarbonate + L-Arg | - |
? | |
benzyloxycarbonyl-Leu-Ala-methyl ester + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
benzyloxycarbonyl-Leu-Gly-methyl ester + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
benzyloxycarbonyl-Phe + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
benzyloxycarbonyl-Phe-Leu + H2O | - |
Saccharolobus solfataricus | benzyloxycarbonyl-Phe + Leu | - |
? | |
benzyloxycarbonyl-Phe-Leu + H2O | - |
Saccharolobus solfataricus P2 | benzyloxycarbonyl-Phe + Leu | - |
? | |
benzyloxycarbonyl-Tyr-ethyl ester + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
additional information | enzyme also shows esterase activity, no activity with 4-nitroanilide substrates | Saccharolobus solfataricus | ? | - |
? | |
additional information | enzyme also shows esterase activity, no activity with 4-nitroanilide substrates | Saccharolobus solfataricus P2 | ? | - |
? | |
peptide + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
peptide + H2O | broad substrate specificity, overview | Saccharolobus solfataricus | ? | - |
? | |
peptide + H2O | - |
Saccharolobus solfataricus P2 | ? | - |
? | |
peptide + H2O | broad substrate specificity, overview | Saccharolobus solfataricus P2 | ? | - |
? | |
protein + H2O | - |
Saccharolobus solfataricus | peptides | - |
? | |
protein + H2O | broad substrate specificity, overview | Saccharolobus solfataricus | peptides | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 42000, SDS-PAGE | Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
carboxypeptidase S1 | - |
Saccharolobus solfataricus |
M20.008 | Merops-ID | Saccharolobus solfataricus |
thermostable carboxypeptidase 1 | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
assay at | Saccharolobus solfataricus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 85 | linear increase, still active at room temperature | Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
25 | instable at, glycerol stabilizes | Saccharolobus solfataricus |
85 | - |
stable for 15 min | Saccharolobus solfataricus |
90 | - |
85% remaining activity | Saccharolobus solfataricus |
95 | - |
17% remaining activity | Saccharolobus solfataricus |
100 | - |
inactivation | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.97 | - |
benzoyl-glycyl-arginine | pH 6.5, 60°C | Saccharolobus solfataricus | |
3.4 | - |
benzyloxycarbonyl-Phe | pH 6.5, 60°C | Saccharolobus solfataricus | |
6 | - |
Benzyloxycarbonyl-Asp | pH 6.5, 60°C | Saccharolobus solfataricus | |
10.4 | - |
Benzyloxycarbonyl-Arg | pH 6.5, 60°C | Saccharolobus solfataricus | |
16.6 | - |
benzoyl-glycyl-arginine | pH 6.5, 60°C | Saccharolobus solfataricus | |
21.2 | - |
benzoyl-glycyl-lysine | pH 6.5, 60°C | Saccharolobus solfataricus | |
36.4 | - |
benzyloxycarbonyl-Gly-Gly-Phe | pH 6.5, 60°C | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | broad optimum, assay at pH 6.5 | Saccharolobus solfataricus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Saccharolobus solfataricus | isoelectric focusing | - |
5.9 |