Literature summary for 3.4.17.6 extracted from

Levy, C.C.; Goldman, P.
Bacterial peptidases. 3. An enzyme specific for N-acyl linkages to alanine (1969), J. Biol. Chem., 244, 4467-4472.

Inhibitors

Inhibitors	Comment	Organism
Benzoyl-Ala	at least 30% inhibition at 0.3 mM; competitive, Ki: 0.009 mM	Soil bacterium
benzoyl-Leu-Ala	at least 30% inhibition at 0.3 mM	Soil bacterium
carboxyl-terminal Ala containing compounds	-	Soil bacterium
Gly-Ala	non-competitive, Ki: 0.13 mM	Soil bacterium
His-Ala	non-competitive, Ki: 0.34 mM	Soil bacterium
additional information	not: compounds containing carboxyl-terminal Met or Gly	Soil bacterium
Phe-Ala	at least 30% inhibition at 0.3 mM	Soil bacterium
phosphate buffer	-	Soil bacterium
Tyr-Ala	at least 30% inhibition at 0.3 mM	Soil bacterium

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
4.3	-	N-benzoyl-L-aminobutyric acid	-	Soil bacterium
4.3	-	N-Benzoyl-Ala	-	Soil bacterium
6.7	-	N-benzoyl-Gly	-	Soil bacterium

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	no effect: Co2+, Mn2+, Mg2+, Fe3+, Fe2+, Cu2+, Cd2+, Ni2+	Soil bacterium
Zn2+	required	Soil bacterium

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
4-aminobenzoylalanine + H2O	Soil bacterium	-	?	-	?
peptidyl-L-alanine + H2O	Soil bacterium	peptide can be replaced by a variety of pteroyl or acyl groups, absolute specificity for alanine in carboxyl-terminal position	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Soil bacterium	-	unidentified	-

Purification (Commentary)

Purification (Comment)	Organism
-	Soil bacterium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
8.3	-	-	Soil bacterium

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
4-amino-4-deoxypteroyl-Ala + H2O	-	Soil bacterium	4-amino-4-deoxypteroate + Ala	-	ir
4-aminobenzoyl-Ala + H2O	-	Soil bacterium	4-aminobenzoate + Ala	-	ir
4-aminobenzoylalanine + H2O	-	Soil bacterium	?	-	?
acyl-Ala + H2O	-	Soil bacterium	? + Ala	-	ir
Ala-Ala + H2O	-	Soil bacterium	Ala + Ala	-	ir
benzoyl-Ala + H2O	-	Soil bacterium	benzoate + Ala	-	ir
benzyloxycarbonyl-Ala + H2O	-	Soil bacterium	benzyloxy-CHO + Ala	-	ir
benzyloxycarbonyl-Ala-Ala + H2O	-	Soil bacterium	benzyloxycarbonyl-Ala + Ala	-	ir
benzyloxycarbonyl-Asp-Ala + H2O	-	Soil bacterium	benzyloxycarbonyl-Asp + Ala	-	ir
benzyloxycarbonyl-Ile-Ala + H2O	-	Soil bacterium	benzyloxycarbonyl-Ile + Ala	-	ir
benzyloxycarbonyl-Leu-Ala + H2O	-	Soil bacterium	benzyloxycarbonyl-Leu + Ala	-	ir
benzyloxycarbonyl-Met-Ala + H2O	-	Soil bacterium	benzyloxycarbonyl-Met + Ala	-	ir
benzyloxycarbonyl-Phe-Ala + H2O	-	Soil bacterium	benzyloxycarbonyl-Phe + Ala	-	ir
benzyloxycarbonyl-Ser-Ala + H2O	-	Soil bacterium	benzyloxycarbonyl-Ser + Ala	-	ir
Glu-Ala + H2O	-	Soil bacterium	Glu + Ala	-	ir
Ile-Ala + H2O	-	Soil bacterium	Ile + Ala	-	ir
Leu-Ala + H2O	-	Soil bacterium	Leu + Ala	-	ir
Met-Ala + H2O	-	Soil bacterium	Met + Ala	-	ir
N-benzoyl-Gly-Ala + H2O	-	Soil bacterium	N-benzoyl-Gly + Ala	-	?
N-benzoyl-L-aminobutyric acid + H2O	-	Soil bacterium	benzoate + L-aminobutyric acid	-	?
peptidyl-L-alanine + H2O	peptide can be replaced by a variety of pteroyl or acyl groups, absolute specificity for alanine in carboxyl-terminal position	Soil bacterium	?	-	?
Phe-Ala + H2O	-	Soil bacterium	Phe + Ala	-	ir
Ser-Ala + H2O	-	Soil bacterium	Ser + Ala	-	ir
Tyr-Ala + H2O	-	Soil bacterium	Tyr + Ala	-	ir

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Soil bacterium

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	8	-	Soil bacterium

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism
0.009	-	Benzoyl-Ala	-	Soil bacterium
0.13	-	Gly-Ala	non-competitive	Soil bacterium
0.34	-	His-Ala	non-competitive	Soil bacterium