Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | no effect by addition of FAD, FMN, NADPH, NADP+, NADH, NAD+ | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 0.1 mM and 1 mM, 95.6% and 74.7% compared to a control without additives | Bos taurus | |
Ag+ | AgNO3, 0.1 mM and 1 mM, complete inhibition | Bos taurus | |
CuSO4 | complete inhibition at 1 mM, 39% inhibition at 0.1 mM | Bos taurus | |
diisopropylphosphorofluoridate | 0.1 mM and 1 mM, 32.3% and 24.3% compared to a control without additives | Bos taurus | |
EDTA | 0.1 mM and 1 mM, 105% and 96.1% compared to a control without additives | Bos taurus | |
iodoacetate | 0.1 mM and 1 mM, 110.5% and 94.8% compared to a control without additives | Bos taurus | |
p-chloromercuriphenylsulfonic acid | 0.1 mM and 1 mM, complete inhibition | Bos taurus | |
PMSF | 0.1 mM and 1 mM, 78.1% and 21.4% compared to a control without additives | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0211 | - |
4-dimethylaminoazobenzene-4'-sulfonyl-Gly-L-Phe-Gly | - |
Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | 0.1 mM and 1 mM, 108.9% and 105.5% compared to a control without additives | Bos taurus | |
MgCl2 | 0.1 mM and 1 mM, 109.8% and 103.4% compared to a control without additives | Bos taurus | |
Mn2+ | MnCl2, 0.1 mM and 1 mM, 106.3% and 104.2% compared to a control without additives | Bos taurus | |
Zn2+ | ZnSO4, 0.1 mM and 1 mM, 97.6% and 69.6% compared to a control without additives | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Mus musculus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Mus musculus | - |
hypophysis | - |
Bos taurus | - |
kidney | - |
Mus musculus | - |
liver | - |
Mus musculus | - |
lung | - |
Mus musculus | - |
spleen | - |
Mus musculus | - |
testis | - |
Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000000113 | - |
six-week-old animal | Mus musculus |
0.000000115 | - |
six-week-old animal | Mus musculus |
0.000000116 | - |
six-week-old animal | Mus musculus |
0.000001917 | - |
six-week-old animal | Mus musculus |
0.00000415 | - |
six-week-old animal | Mus musculus |
0.00000845 | - |
six-week-old animal | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-dimethylaminoazobenzene-4'-sulfonyl-Gly-L-Phe-Gly + H2O | - |
Mus musculus | 4-dimethylaminoazobenzene-4'-sulfonyl-Gly-L-Phe | enzyme assay is based on the detection of the product using HPLC to separate it from the substrate | ? | |
4-dimethylaminoazobenzene-4'-sulfonyl-Gly-L-Phe-Gly + H2O | - |
Bos taurus | 4-dimethylaminoazobenzene-4'-sulfonyl-Gly-L-Phe | enzyme assay is based on the detection of the product using HPLC to separate it from the substrate | ? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.8 | 5.4 | 4-dimethylaminoazobenzene-4'-sulfonyl-Gly-L-Phe-Gly as substrate, 50 mM sodium acetate buffer | Bos taurus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.2 | 6.5 | pH 4.2: 28% of the maximal activity, pH 6.5: 43% of the maximal activity | Bos taurus |