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Literature summary for 3.4.17.4 extracted from

  • Watson, H.; Bonifacino, J.S.
    Direct binding to Rsp5p regulates ubiquitination-independent vacuolar transport of Sna3p (2007), Mol. Biol. Cell, 18, 1781-1789.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.17.4

Application

Application Comment Organism
additional information sorting of Cps1p into the luminal vesicles of multivesicular bodies requires ubiquitination of their cytosolic domains by the ubiquitin ligases Rsp5p and/or Tul1p, whereas Sna3p, another integral membrane protein, does not require ubiquitination for entry into multivesicular bodies. Sna3p follows an ubiquitination-independent, but Rsp5p-mediated, sorting pathway to the vacuole Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment) Organism
GFP-Cps1p expressed in rsp5delta cells expressing wild-type or mutant HA-Rsp5p with WW domain mutations W257A, W359A, and W415A. GFP-Cps1p expressed in rsp5delta cells expressing HARsp5pWT, HA-rsp5pL733S or HA-rsp5G555D. GFP-Cps1p expressed in tul1delta cells, bsd2delta cells and sna3delta cells Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
additional information GFP-Cps1p accumulates in a prevacuolar compartment as well as on the limiting membrane of the vacuole in rps5WW1-3 cells with WW domain mutations. In rsp5L733S cells, GFP-Cps1p is detectable in the lumen of the vacuole, some is also found on the limiting membrane of the vacuole. In bsd2delta cells, GFP-Cps1p accumulates in the limiting membrane of the vacuole. In tul1delta cells sorting is also impaired. No defect in GFP-Cps1p vacuolar sorting in sna3delta cells Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane sorting of integral membrane protein Cps1p into the luminal vesicles of multivesicular bodies Saccharomyces cerevisiae 16020
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Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
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Synonyms

Synonyms Comment Organism
carboxypeptidase S
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 Saccharomyces cerevisiae
Cps1p
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 Saccharomyces cerevisiae