General Stability | Organism |
---|---|
plasmin and trypsin cleave the enzyme to lower molecular weight fragments | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
hippuryl-Arg | - |
Homo sapiens | |
0.25 | - |
Benzoyl-Ala-Lys | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
2 * 48000 + 2 * 83000, the 48000 Da subunit is as active as the intact enzyme, the 83000 Da subunit is inactive but stabilizes the 48000 Da subunit, SDS-PAGE | Homo sapiens |
83000 | - |
2 * 48000 + 2 * 83000, the 48000 Da subunit is as active as the intact enzyme, the 83000 Da subunit is inactive but stabilizes the 48000 Da subunit, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
side-chain modification | the 83000 Da subunit contains carbohydrate | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
plasma | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg + H2O | - |
Homo sapiens | Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe + Arg | - |
? | |
benzoyl-Ala-Lys + H2O | - |
Homo sapiens | ? | - |
? | |
Hippuryl-L-Arg + H2O | - |
Homo sapiens | Hippuric acid + L-Arg | - |
? | |
kallikrein + H2O | plasma or urinary | Homo sapiens | ? | - |
? | |
plasmin + H2O | - |
Homo sapiens | ? | - |
? | |
trypsin + H2O | - |
Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 2 * 48000 + 2 * 83000, the 48000 Da subunit is as active as the intact enzyme, the 83000 Da subunit is inactive but stabilizes the 48000 Da subunit, SDS-PAGE | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
room temperature, pH 4-5, 1 h, the 48000 Da subunit loses 94% of its activity, the intact enzyme loses 9% of its activity | Homo sapiens |
37 | - |
2 h, stable | Homo sapiens |