Cloned (Comment) | Organism |
---|---|
expression in PC3 cell | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-(phosphonomethyl) pentanedioic acid | inhibitor completely blocks N-acetylaspartylglutamate cleavage activity but not Amyloid-beta degradation | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q04609 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
U-87MG cell | - |
Homo sapiens | - |
General Information | Comment | Organism |
---|---|---|
physiological function | glutamate carboxypeptidase II has a physiological function in degrading amyloid-beta. Amyloid-beta degradation occurs through S1 pocket but not through S1' pocket responsible for N-acetylaspartylglutamate hydrolysis. Treatment with a S1 pocket-specific chemical inhibitor prevents GCPII from amyloid-beta degradation without any impairment in N-acetylaspartylglutamate hydrolysis. Likewise, specific GCPII inhibitor 2-(phosphonomethyl) pentanedioic acid developed targeting S1' pocket completely blocks the N-acetylaspartylglutamate hydrolysis without any effect on amyloid-beta degradation. Pre-incubation with N-acetylaspartylglutamate and amyloid-beta does not affect amyloid-beta degradation and N-acetylaspartylglutamate hydrolysis, respectively | Homo sapiens |