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Literature summary for 3.4.17.20 extracted from

  • Schneider, M.; Nagashima, M.; Knappe, S.; Zhao, L.; Morser, J.; Nesheim, M.
    Amino acid residues in the P6-P'3 region of thrombin-activable fibrinolysis inhibitor (TAFI) do not determine the thrombomodulin dependence of TAFI activation (2002), J. Biol. Chem., 277, 9944-9951.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in BHK cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
D87A activated with similar kinetics as wild-type enzyme by thrombin-thrombomodulin. Increase in activation by plasmin. Thermal inactivation at 37°C is similar to that of the wild-type enzyme Homo sapiens
additional information mutant with the fibrinogen Bbeta thrombin cleavage site Ala-Arg-Gly-His-Arg substituted into positions 91-95: activated with similar kinetics as wild-type enzyme by thrombin-thrombomodulin, activated by plasmin with the same kinetics as wild-type enzyme. Mutant enzyme with the protein C thrombin cleavage site Asp-Pro-Arg-Leu-Ile-Asp substituted into positions 90-95: no measurable activation by plasmin Homo sapiens
S90A activated with similar kinetics as wild-type enzyme by thrombin-thrombomodulin. Increase in activation by plasmin. Thermal inactivation at 37°C is similar to that of the wild-type enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.979
-
anisylazoformyl-Lys 24°C Homo sapiens
1.089
-
anisylazoformyl-Lys 24°C Homo sapiens
1.11
-
anisylazoformyl-Lys 24°C Homo sapiens
1.26
-
anisylazoformyl-Lys 24°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
x * 60000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
fibrin + H2O Homo sapiens attenuates clot lysis by removing lysine residues from a fibrin clot Lys + ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Homo sapiens
-
variant A147/I325
-
Homo sapiens
-
variant A147/T325
-
Homo sapiens
-
variant T147/I325
-
Homo sapiens
-
variant T147/T325
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the TAFI zymogen is activated to TAFIa by plasmin, thrombin, or the thrombin-thrombomodulin complex. Turnover-number for TAFI activation by solunin in presence of throbin is 0.74 per s, the Km-value is 0.00067 mM Homo sapiens
proteolytic modification the TAFI zymogen is activated to TAFIa by plasmin, thrombin, or the thrombin-thrombomodulin complex. Turnover-number for TAFI activation by solunin in presence of throbin is 1.06 per s, the Km-value is 0.00084 mM Homo sapiens
proteolytic modification the TAFI zymogen is activated to TAFIa by plasmin, thrombin, or the thrombin-thrombomodulin complex. Turnover-number for TAFI activation by solunin in presence of thrombin is 0.74 per s, the Km-value is 0.00082 mM Homo sapiens
proteolytic modification the TAFI zymogen is activated to TAFIa by plasmin, thrombin, or the thrombin-thrombomodulin complex. Turnover-number for TAFI activation by solunin in presence of thrombin is 0.85 per s, the Km-value is 0.00083 mM Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
wild-type and mutant enzymes Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
anisylazoformyl-Lys + H2O
-
Homo sapiens anisylazoformic acid + Lys
-
?
fibrin + H2O attenuates clot lysis by removing lysine residues from a fibrin clot Homo sapiens Lys + ?
-
?

Subunits

Subunits Comment Organism
? x * 60000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
TAFI
-
Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
half-life: 147 min Homo sapiens
25
-
half-life: 162 min Homo sapiens
25
-
half-life: 73.8 min Homo sapiens
25
-
half-life: 77.2 min Homo sapiens
30
-
half-life: 54 min Homo sapiens
30
-
half-life: 28.3 min Homo sapiens
30
-
half-life: 28.6 min Homo sapiens
33
-
half-life: 14.5 min Homo sapiens
33
-
half-life: 15.4 min Homo sapiens
33
-
half-life: 27.2 min Homo sapiens
33
-
half-life: 27.4 min Homo sapiens
37
-
half-life: 15 min Homo sapiens
37
-
half-life: 14.7 min Homo sapiens
37
-
half-life: 7.8 min Homo sapiens
37
-
half-life: 7.8 min for wild-type enzyme, 7.6 min for mutant enzyme S90A and 7.5 min for mutant enzyme D87A Homo sapiens
37
-
half-life: 8.0 min Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
15.9
-
anisylazoformyl-Lys 24°C Homo sapiens
26.8
-
anisylazoformyl-Lys 24°C Homo sapiens
27
-
anisylazoformyl-Lys 24°C Homo sapiens
27.4
-
anisylazoformyl-Lys 24°C Homo sapiens
29.5
-
anisylazoformyl-Lys 24°C Homo sapiens