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Literature summary for 3.4.17.20 extracted from
- Plasma and recombinant thrombin-activable fibrinolysis inhibitor (TAFI) and activated TAFI compared with respsct to glycosylation, thrombin/thrombomodulin-dependent activation, thermal stability, and enzymatic properties (1998), J. Biol. Chem., 273, 2127-2135.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| thrombin | activation | Homo sapiens | |
| Thrombomodulin | activation | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in BHK-cells | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-Guanidinoethylmercaptosuccinic acid | i.e. GEMSA | Homo sapiens |  |
| 6-aminohexanoic acid | - |
Homo sapiens | |
| potato carboxypeptidase inhibitor | - |
Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.41 | - |
N-[3-(2-Furylacryloyl)]-L-Ala-L-Lys | recombinant enzyme | Homo sapiens | |
| 4.71 | - |
N-[3-(2-Furylacryloyl)]-L-Ala-L-Lys | plasma-derived enzyme | Homo sapiens | |
| 766 | - |
Hippuryl-L-Lys | plasma-derived enzyme | Homo sapiens | |
| 783 | - |
hippuryl-L-Arg | recombinant enzyme | Homo sapiens | |
| 817 | - |
hippuryl-L-Arg | plasma-derived enzyme | Homo sapiens | |
| 933 | - |
Hippuryl-L-Lys | recombinant enzyme | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 12000 | - |
x * 25000 + x * 12000, derived by cleavage at Arg330, inactive form, SDS-PAGE | Homo sapiens |
| 25000 | - |
x * 25000 + x * 12000, derived by cleavage at Arg330, inactive form, SDS-PAGE | Homo sapiens |
| 35000 | - |
x * 35000, active enzyme | Homo sapiens |
| 46000 | - |
x * 46000, enzymes rTAFI and pTAFI, deglycosylated enzymes, slight differences in MW might be due to differences in glycosylation, SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Fibrin, partially degraded + H2O | Homo sapiens | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| of cloned enzyme from BHK-cells | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| plasma | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.017 | - |
substrate hippuryl-L-Arg | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Fibrin, partially degraded + H2O | - |
Homo sapiens | Fibrin, partially degraded + L-Lys + L-Arg | - |
? | |
| Fibrin, partially degraded + H2O | - |
Homo sapiens | ? | - |
? | |
| Hippuryl-L-Arg + H2O | - |
Homo sapiens | Hippuric acid + L-Arg | - |
? | |
| Hippuryl-L-Lys + H2O | - |
Homo sapiens | Hippuric acid + L-Lys | - |
? | |
| N-[3-(2-Furylacryloyl)]-L-Ala-L-Lys + H2O | - |
Homo sapiens | N-[3-(2-Furylacryloyl)]-L-Ala + L-Lys | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | derived from zymogen by cleavage at Arg92, SDS-PAGE | Homo sapiens |
| ? | x * 25000 + x * 12000, derived by cleavage at Arg330, inactive form, SDS-PAGE | Homo sapiens |
| ? | x * 46000, enzymes rTAFI and pTAFI, deglycosylated enzymes, slight differences in MW might be due to differences in glycosylation, SDS-PAGE | Homo sapiens |
| ? | x * 35000, active enzyme | Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
- |
enzyme pTAFI and rTAFI stable | Homo sapiens |
| 22 | - |
half-life 120-150 min | Homo sapiens |
| 30 | - |
half-life 40-50 min | Homo sapiens |
| 37 | - |
half-life 8-9 min, stable in presence of inhibitors 2-guanidinoethylmercaptosuccinic acid or 6-aminohexanoic acid | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 40.7 | - |
Hippuryl-L-Lys | plasma-derived enzyme | Homo sapiens | |
| 45.6 | - |
Hippuryl-L-Lys | recombinant enzyme | Homo sapiens | |
| 52.4 | - |
hippuryl-L-Arg | plasma-derived enzyme | Homo sapiens | |
| 56.7 | - |
hippuryl-L-Arg | recombinant enzyme | Homo sapiens | |
| 248 | - |
N-[3-(2-Furylacryloyl)]-L-Ala-L-Lys | recombinant enzyme | Homo sapiens | |
| 293 | - |
N-[3-(2-Furylacryloyl)]-L-Ala-L-Lys | plasma-derived enzyme | Homo sapiens | |
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