Literature summary for 3.4.17.2 extracted from

Chatterjee, S.; Lardinois, O.; Bonini, M.G.; Bhattacharjee, S.; Stadler, K.; Corbett, J.; Deterding, L.J.; Tomer, K.B.; Kadiiska, M.; Mason, R.P.
Site-specific carboxypeptidase B1 tyrosine nitration and pathophysiological implications following its physical association with nitric oxide synthase-3 in experimental sepsis (2009), J. Immunol., 183, 4055-4066.

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Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	confocal microscopy results indicate that levels of both CPB1 and nitric oxide synthase-3 are high in the septic spleen and that CPB1 colocalizes with nitric oxide synthase-3 in the sinus-lining cells of the red pulp. LPS treatment results in the translocation of nitric oxide synthase-3 to the cytosol and colocalization with CPB1	Mus musculus	5829	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
32000	-	Western blot analysis, investigation of peroxynitrite-induced chemical nitrosylation of CPB	Sus scrofa
35000	-	Western blot analysis, investigation of peroxynitrite-induced chemical nitrosylation of CPB	Sus scrofa
47000	-	Western Blot analysis, nitrosylated CPB1	Mus musculus
70000	-	Western blot analysis, investigation of peroxynitrite-induced chemical nitrosylation of CPB, dimer formation, band immunoreactive to an antibody specific to 3-nitrotyrosine	Sus scrofa

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	-	-	-
Sus scrofa	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
nitrosylation	LPS-induced septic shock produces tyrosine nitration in vivo which is due to the formation of peroxynitrite and is mediated by xanthine oxidase and nitric oxide synthase-3. Nitrosylation leads to the inactivation of the enzyme	Mus musculus
nitrosylation	peroxynitrite-dependent tyrosine nitration leading to inactivation of the enzyme. At least 11 peptides with five sites of nitration on tyrosine residues are identified. These include Tyr92, Tyr210, Tyr277, Tyr248, and Tyr198. Of these, Tyr248 and Tyr198 are located in the catalytic site of CPB	Sus scrofa

Source Tissue

Source Tissue	Comment	Organism	Textmining
spleen	confocal microscopy results indicate that levels of both CPB1 and nitric oxide synthase-3 are high in the septic spleen and that CPB1 colocalizes with nitric oxide synthase-3 in the sinus-lining cells of the red pulp. LPS treatment results in the translocation of nitric oxide synthase-3 to the cytosol and colocalization with CPB1	Mus musculus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Hippuryl-L-Arg + H2O	-	Mus musculus	Hippuric acid + L-Arg	-	?
Hippuryl-L-Arg + H2O	-	Sus scrofa	Hippuric acid + L-Arg	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 35000, SDS-PAGE, dimer formation as a result of dityrosine crosslinks	Sus scrofa

Synonyms

Synonyms	Comment	Organism
carboxypeptidase B	-	Sus scrofa
Carboxypeptidase B1	-	Mus musculus
CPB	-	Sus scrofa
CPB1	-	Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Mus musculus
25	-	assay at	Sus scrofa

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.65	-	assay at	Mus musculus
7.65	-	assay at	Sus scrofa

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Release 2023.1 (January 2023)