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Literature summary for 3.4.17.2 extracted from

  • Aviles, F.X.; Vendrell, J.
    Carboxypeptidase B (2004), Handbook of Proteolytic Enzymes (Barrett, J. ; Rawlings, N. D. ; Woessner, J. F. , eds. ) Academic Press, 2, 831-833.
No PubMed abstract available
Search for more literature for 3.4.17.2

Application

Application Comment Organism
diagnostics the enzyme is a serum marker for acute pancreatitis and pancreatic graft injection Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
DNA sequence determination, phylogenetic tree Homo sapiens
DNA sequence determination, phylogenetic tree Rattus norvegicus
DNA sequence determination, phylogenetic tree Sus scrofa
DNA sequence determination, phylogenetic tree Bos taurus

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptomethyl-3-guanidinoethyl-propanoic acid
-
 Bos taurus
2-mercaptomethyl-3-guanidinoethyl-propanoic acid
-
 Homo sapiens
2-mercaptomethyl-3-guanidinoethyl-propanoic acid
-
 Rattus norvegicus
2-mercaptomethyl-3-guanidinoethyl-propanoic acid
-
 Sus scrofa
2-mercaptomethyl-5-guanidinopentanoic acid
-
 Bos taurus
2-mercaptomethyl-5-guanidinopentanoic acid
-
 Homo sapiens
2-mercaptomethyl-5-guanidinopentanoic acid
-
 Rattus norvegicus
2-mercaptomethyl-5-guanidinopentanoic acid
-
 Sus scrofa
Guanidinoethylmercaptosuccinic acid
-
 Bos taurus
Guanidinoethylmercaptosuccinic acid
-
 Homo sapiens
Guanidinoethylmercaptosuccinic acid
-
 Rattus norvegicus
Guanidinoethylmercaptosuccinic acid
-
 Sus scrofa
leech carboxypeptidase inhibitor
-
 Bos taurus
leech carboxypeptidase inhibitor
-
 Homo sapiens
leech carboxypeptidase inhibitor
-
 Rattus norvegicus
leech carboxypeptidase inhibitor
-
 Sus scrofa
potato carboxypeptidase inhibitor
-
 Bos taurus
potato carboxypeptidase inhibitor
-
 Homo sapiens
potato carboxypeptidase inhibitor
-
 Rattus norvegicus
potato carboxypeptidase inhibitor
-
 Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the enzyme is secreted from pancreas Sus scrofa
-
-
extracellular the enzyme is secreted from pancreas Bos taurus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cl- required Homo sapiens
Cl- required Rattus norvegicus
Cl- required Sus scrofa
Cl- required Bos taurus
Zn2+ 1 mol Zn2+ per mol of enzyme, coordinated by the conserved residues His69, Glu72, and His196 Homo sapiens
Zn2+ 1 mol Zn2+ per mol of enzyme, coordinated by the conserved residues His69, Glu72, and His196 Rattus norvegicus
Zn2+ 1 mol Zn2+ per mol of enzyme, coordinated by the conserved residues His69, Glu72, and His196 Sus scrofa
Zn2+ 1 mol Zn2+ per mol of enzyme, coordinated by the conserved residues His69, Glu72, and His196 Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens the enzyme is highly specific for excising C-terminal Lys and Arg residues from peptides and proteins with a preference for Arg, it also acts on C-terminal Val, Leu, Ile, Asn, Gly, and Gln but at a slower rate, the enzyme shows a preference for Ala in P2 position ?
-
 ?
additional information Rattus norvegicus the enzyme is highly specific for excising C-terminal Lys and Arg residues from peptides and proteins with a preference for Arg, it also acts on C-terminal Val, Leu, Ile, Asn, Gly, and Gln but at a slower rate, the enzyme shows a preference for Ala in P2 position ?
-
 ?
additional information Sus scrofa the enzyme is highly specific for excising C-terminal Lys and Arg residues from peptides and proteins with a preference for Arg, it also acts on C-terminal Val, Leu, Ile, Asn, Gly, and Gln but at a slower rate, the enzyme shows a preference for Ala in P2 position ?
-
 ?
additional information Bos taurus the enzyme is highly specific for excising C-terminal Lys and Arg residues from peptides and proteins with a preference for Arg, it also acts on C-terminal Val, Leu, Ile, Asn, Gly, and Gln but at a slower rate, the enzyme shows a preference for Ala in P2 position ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Homo sapiens
-
-
-
Rattus norvegicus
-
-
-
Sus scrofa
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the enzyme is synthesized as zymogen, cleavage and activation in vitro by trypsin Homo sapiens
proteolytic modification the enzyme is synthesized as zymogen, cleavage and activation in vitro by trypsin Rattus norvegicus
proteolytic modification the enzyme is synthesized as zymogen, cleavage and activation in vitro by trypsin Sus scrofa
proteolytic modification the enzyme is synthesized as zymogen, cleavage and activation in vitro by trypsin Bos taurus

Purification (Commentary)

Purification (Comment) Organism
native enzyme from pancreatic acetine powder Bos taurus

Reaction

Reaction Comment Organism Reaction ID
preferential release of a C-terminal lysine or arginine amino acid conserved catalytic residues are Glu270 and Asp127, residues Ser207, Glu243, and Asp255 determine the substrate specificity Homo sapiens
a
preferential release of a C-terminal lysine or arginine amino acid conserved catalytic residues are Glu270 and Asp127, residues Ser207, Glu243, and Asp255 determine the substrate specificity Rattus norvegicus
a
preferential release of a C-terminal lysine or arginine amino acid conserved catalytic residues are Glu270 and Asp127, residues Ser207, Glu243, and Asp255 determine the substrate specificity Sus scrofa
a
preferential release of a C-terminal lysine or arginine amino acid conserved catalytic residues are Glu270 and Asp127, residues Ser207, Glu243, and Asp255 determine the substrate specificity Bos taurus
a

Source Tissue

Source Tissue Comment Organism Textmining
intestine
-
 Bos taurus
-
muscle longitudinal, of instestine Bos taurus
-
pancreas
-
 Homo sapiens
-
pancreas
-
 Sus scrofa
-
pancreas
-
 Bos taurus
-
serum
-
 Homo sapiens
-

Storage Stability

Storage Stability Organism
frozen or precipitated purified pancreatic enzyme is stable for years Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzoyl-Gly-Arg + H2O
-
 Homo sapiens benzoyl-Gly + Arg
-
 ?
benzoyl-Gly-Arg + H2O
-
 Rattus norvegicus benzoyl-Gly + Arg
-
 ?
benzoyl-Gly-Arg + H2O
-
 Sus scrofa benzoyl-Gly + Arg
-
 ?
benzoyl-Gly-Arg + H2O
-
 Bos taurus benzoyl-Gly + Arg
-
 ?
additional information the enzyme is highly specific for excising C-terminal Lys and Arg residues from peptides and proteins with a preference for Arg, it also acts on C-terminal Val, Leu, Ile, Asn, Gly, and Gln but at a slower rate, the enzyme shows a preference for Ala in P2 position Homo sapiens ?
-
 ?
additional information the enzyme is highly specific for excising C-terminal Lys and Arg residues from peptides and proteins with a preference for Arg, it also acts on C-terminal Val, Leu, Ile, Asn, Gly, and Gln but at a slower rate, the enzyme shows a preference for Ala in P2 position Rattus norvegicus ?
-
 ?
additional information the enzyme is highly specific for excising C-terminal Lys and Arg residues from peptides and proteins with a preference for Arg, it also acts on C-terminal Val, Leu, Ile, Asn, Gly, and Gln but at a slower rate, the enzyme shows a preference for Ala in P2 position Sus scrofa ?
-
 ?
additional information the enzyme is highly specific for excising C-terminal Lys and Arg residues from peptides and proteins with a preference for Arg, it also acts on C-terminal Val, Leu, Ile, Asn, Gly, and Gln but at a slower rate, the enzyme shows a preference for Ala in P2 position Bos taurus ?
-
 ?
additional information the enzyme is highly specific for excising C-terminal Lys and Arg residues from peptides and proteins with a preference for Arg, it also acts on C-terminal Val, Leu, Ile, Asn, Gly, and Gln but at a slower rate, the enzyme shows a preference for Ala in P2 position, the enzyme shows no intrinsic carboxypeptidase and autoactivation activity Homo sapiens ?
-
 ?
additional information the enzyme is highly specific for excising C-terminal Lys and Arg residues from peptides and proteins with a preference for Arg, it also acts on C-terminal Val, Leu, Ile, Asn, Gly, and Gln but at a slower rate, the enzyme shows a preference for Ala in P2 position, the enzyme shows no intrinsic carboxypeptidase and autoactivation activity Rattus norvegicus ?
-
 ?
additional information the enzyme is highly specific for excising C-terminal Lys and Arg residues from peptides and proteins with a preference for Arg, it also acts on C-terminal Val, Leu, Ile, Asn, Gly, and Gln but at a slower rate, the enzyme shows a preference for Ala in P2 position, the enzyme shows no intrinsic carboxypeptidase and autoactivation activity Sus scrofa ?
-
 ?
additional information the enzyme is highly specific for excising C-terminal Lys and Arg residues from peptides and proteins with a preference for Arg, it also acts on C-terminal Val, Leu, Ile, Asn, Gly, and Gln but at a slower rate, the enzyme shows a preference for Ala in P2 position, the enzyme shows no intrinsic carboxypeptidase and autoactivation activity Bos taurus ?
-
 ?

Subunits

Subunits Comment Organism
More the pancreatic enzyme is divided in the activation domain and the enzyme domain linked by a connecting region Homo sapiens
More the pancreatic enzyme is divided in the activation domain and the enzyme domain linked by a connecting region Rattus norvegicus
More the pancreatic enzyme is divided in the activation domain and the enzyme domain linked by a connecting region Bos taurus
More the pancreatic enzyme is divided in the activation domain, residues 1-80, and the enzyme domain, residues 96-401, linked by a connecting region, residues 81-95 Sus scrofa

Synonyms

Synonyms Comment Organism
basic carboxypeptidase
-
 Homo sapiens
basic carboxypeptidase
-
 Rattus norvegicus
basic carboxypeptidase
-
 Sus scrofa
basic carboxypeptidase
-
 Bos taurus
CPB
-
 Homo sapiens
CPB
-
 Rattus norvegicus
CPB
-
 Sus scrofa
CPB
-
 Bos taurus
More the enzyme belongs to the M14A peptidase family Homo sapiens
More the enzyme belongs to the M14A peptidase family Rattus norvegicus
More the enzyme belongs to the M14A peptidase family Sus scrofa
More the enzyme belongs to the M14A peptidase family Bos taurus
protaminase formerly Homo sapiens
protaminase formerly Rattus norvegicus
protaminase formerly Sus scrofa
protaminase formerly Bos taurus
tissue carboxypeptidase B
-
 Homo sapiens
tissue carboxypeptidase B
-
 Rattus norvegicus
tissue carboxypeptidase B
-
 Sus scrofa
tissue carboxypeptidase B
-
 Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 7.8 assay at Homo sapiens
7.5 7.8 assay at Rattus norvegicus
7.5 7.8 assay at Sus scrofa
7.5 7.8 assay at Bos taurus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information the Ki values for 2-mercaptomethyl-3-guanidinoethyl-propanoic acid, potato and leech carboxypeptidase inhibitors, and 2-mercaptomethyl-5-guanidinopentanoic acid are in the nanomolar range Homo sapiens
additional information
-
 additional information the Ki values for 2-mercaptomethyl-3-guanidinoethyl-propanoic acid, potato and leech carboxypeptidase inhibitors, and 2-mercaptomethyl-5-guanidinopentanoic acid are in the nanomolar range Rattus norvegicus
additional information
-
 additional information the Ki values for 2-mercaptomethyl-3-guanidinoethyl-propanoic acid, potato and leech carboxypeptidase inhibitors, and 2-mercaptomethyl-5-guanidinopentanoic acid are in the nanomolar range Sus scrofa
additional information
-
 additional information the Ki values for 2-mercaptomethyl-3-guanidinoethyl-propanoic acid, potato and leech carboxypeptidase inhibitors, and 2-mercaptomethyl-5-guanidinopentanoic acid are in the nanomolar range Bos taurus