Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Subtilisin | cleavage of the carboxypeptidase precursor | Thermoactinomyces vulgaris |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermoactinomyces vulgaris |
General Stability | Organism |
---|---|
Ca2+ important for thermostability | Thermoactinomyces vulgaris |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 10 mM, required for enzyme stabilization | Thermoactinomyces vulgaris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
SDS-PAGE, mature enzyme | Thermoactinomyces vulgaris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoactinomyces vulgaris | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purification from inclusion bodes | Thermoactinomyces vulgaris |
Renatured (Comment) | Organism |
---|---|
best conditions: 30 mM Tris-HCl pH 9.0, 0.5 M NaCl, 30% glycerol, 2 mM dithiothreitol, 2 mM cysteine, 10 mM CaCl2, 40°C for 12 h | Thermoactinomyces vulgaris |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Thermoactinomyces vulgaris | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.2 | - |
0.2 ml 50 mM Tris-HCl pH 8.0, 5 mM CaCl2, 0.2 ml 2,4-dinitrophenyl-Ala-Ala-Arg, 37°C | Thermoactinomyces vulgaris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-dinitrophenyl-Ala-Ala-Arg + H2O | - |
Thermoactinomyces vulgaris | 2,4-dinitrophenyl-Ala-Ala + Arg | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 38000, SDS-PAGE, mature enzyme | Thermoactinomyces vulgaris |
Synonyms | Comment | Organism |
---|---|---|
carboxypeptidase T | - |
Thermoactinomyces vulgaris |
CPT | - |
Thermoactinomyces vulgaris |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
10% denatured protein after 2 h at 65°C, 30 mM Tris-HCl pH 9.0, 0.5 M NaCl, 30% glycerol, 2 mM dithiothreitol, 2 mM cysteine, 10 mM CaCl2, rapid denaturing occurs without CaCl2 under the same conditions | Thermoactinomyces vulgaris |