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Literature summary for 3.4.17.13 extracted from
- LD-Carboxypeptidase activity in Escherichia coli. II. Isolation, purification and characterization of the enzyme from E. coli K 12 (1986), Arch. Microbiol., 144, 181-186.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Azthreonam | - |
Escherichia coli |  |
| Ca2+ | 10 mM | Escherichia coli | |
| D-Amino acids | - |
Escherichia coli | |
| Mg2+ | 10 mM | Escherichia coli | |
| Mn2+ | 10 mM | Escherichia coli | |
| nocardicin A | - |
Escherichia coli | |
| Zn2+ | 10 mM | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.108 | - |
UDP-N-acetylmuramyl-Ala-D-Glu-Dap-D-Ala | - |
Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 11800 | - |
E. coli, gel filtration | Escherichia coli |
| 12000 | - |
1 * 12000, SDS-PAGE | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
K12 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| K12 | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -85°C, protein concentration: 0.5 mg/ml, stable for 4 months | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-Acetylglucosamine-N-acetylmuramyl-Ala-D-Glu-Dap-D-Ala + H2O | - |
Escherichia coli | N-Acetylglucosamine-N-acetylmuramyl-Ala-D-Glu-Dap + D-Ala | - |
? | |
| N-acetylmuramoyl-Ala-D-Glu-Dap-D-Ala + H2O | - |
Escherichia coli | N-acetylmuramoyl-Ala-D-Glu-Dap + D-Ala | - |
? | |
| UDP-N-acetylmuramyl-Ala-D-Glu-Dap-D-Ala + H2O | - |
Escherichia coli | UDP-N-acetylmuramyl-Ala-D-Glu-Dap + D-Ala | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 12000, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Escherichia coli |
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