Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.17.11 extracted from

  • Jamin, Y.; Gabellieri, C.; Smyth, L.; Reynolds, S.; Robinson, S.P.; Springer, C.J.; Leach, M.O.; Payne, G.S.; Eykyn, T.R.
    Hyperpolarized (13)C magnetic resonance detection of carboxypeptidase G2 activity (2009), Magn. Reson. Med., 62, 1300-1304.
    View publication on PubMed

Application

Application Comment Organism
medicine carboxypeptidase G2 is a bacterial enzyme that is currently employed in a range of targeted cancer chemotherapy strategies such as gene-directed enzyme prodrug therapy (GDEPT). These therapeutic strategies would benefit from robust imaging strategies that afford high spatial and temporal resolution images of the biodistribution of carboxypeptidase G2 activity. Employing dynamic nuclear polarization (DNP) and natural abundance 13C magnetic resonance spectroscopy (MRS), the carboxypeptidase G2-mediated conversion of a novel hyperpolarized reporter probe 3,5-difluorobenzoyl-L-glutamic acid to 3,5-difluorobenzoic acid and L-glutamic acid is observed in vitro. The potential for translation in vivo will primarily depend on the 13C relaxation times and this appears to be an important limiting factor in the case of 3,5-difluorobenzoyl-L-glutamic acid Pseudomonas sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Pseudomonas sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+
-
Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
Pseudomonas sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,5-difluorobenzoyl-L-glutamic acid + H2O
-
Pseudomonas sp. 3,5-difluorobenzoic acid + L-glutamate
-
?

Synonyms

Synonyms Comment Organism
carboxypeptidase G2
-
Pseudomonas sp.
CPG2
-
Pseudomonas sp.