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Literature summary for 3.4.17.11 extracted from

  • Friedlos, F.; Lehouritis, P.; Ogilvie, L.; Hedley, D.; Davies, L.; Bermudes, D.; King, I.; Martin, J.; Marais, R.; Springer, C.J.
    Attenuated Salmonella targets prodrug activating enzyme carboxypeptidase G2 to mouse melanoma and human breast and colon carcinomas for effective suicide gene therapy (2008), Clin. Cancer Res., 14, 4259-4266.
    View publication on PubMed
Search for more literature for 3.4.17.11

Application

Application Comment Organism
medicine CPG2 possesses several properties that make it suitable for suicide gene therapy Pseudomonas sp.

Cloned(Commentary)

Cloned (Comment) Organism
for expression in Salmonella typhimurium cells using the vector pTrc99A Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+
-
 Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 monomer, determined by immunoblotting Pseudomonas sp.
83000
-
-
 Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pseudomonas sp. CPG2 cleaves folic acid to pteroic acid and activates a range of bifunctional alkylating agent and antibiotic prodrugs ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 0.5 U/ml Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,5-difluoro-4-[bis(2-iodoethyl)amino]benzoic acid + H2O cognate drug Pseudomonas sp. ?
-
 ?
3,5-difluoro-4-[bis(2-iodoethyl)amino]benzoyl-L-glutamic acid + H2O prodrug activated by CPG2 Pseudomonas sp. ?
-
 ?
3-fluoro-4-[bis(2-chloroethyl)amino]benzoyl-L-glutamic acid + H2O prodrug activated by CPG2 Pseudomonas sp. ?
-
 ?
4-[(2-chloroethyl)(2-methylsulphonyloxyethyl)amino]benzoic acid + H2O cognate drug Pseudomonas sp. ?
-
 ?
4-[(2-chloroethyl)-(2-methylsulphonyloxyethyl)amino]benzoyl-L-glutamic acid + H2O prodrug activated by CPG2 Pseudomonas sp. ?
-
 ?
4-[bis(2-iodoethyl)-amino]-phenyloxycarbonyl-L-glutamic acid + H2O prodrug activated by CPG2 Pseudomonas sp. ?
-
 ?
methotrexate + H2O substrate in activity assay Pseudomonas sp. ?
-
 ?
additional information CPG2 cleaves folic acid to pteroic acid and activates a range of bifunctional alkylating agent and antibiotic prodrugs Pseudomonas sp. ?
-
 ?

Subunits

Subunits Comment Organism
homodimer
-
 Pseudomonas sp.

Synonyms

Synonyms Comment Organism
carboxypeptidase G2
-
 Pseudomonas sp.
CPG2
-
 Pseudomonas sp.