General Stability | Organism |
---|---|
activity can increase up to 30% if the enzyme at all stages of purification is stored at -20°C | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ac-Glu | inhibition of methotrexate hydrolysis | Pseudomonas sp. | |
Ala-Glu | inhibition of methotrexate hydrolysis | Pseudomonas sp. | |
Benzoyl-Glu | inhibition of methotrexate hydrolysis | Pseudomonas sp. | |
Benzyloxycarbonyl-Glu | inhibition of methotrexate hydrolysis | Pseudomonas sp. | |
Benzyloxycarbonyl-Gly-Glu | inhibition of methotrexate hydrolysis | Pseudomonas sp. | |
Glu-Glu | inhibition of methotrexate hydrolysis | Pseudomonas sp. | |
Gly-Glu | inhibition of methotrexate hydrolysis | Pseudomonas sp. | |
His-Glu | inhibition of methotrexate hydrolysis | Pseudomonas sp. | |
Ile-Glu | inhibition of methotrexate hydrolysis | Pseudomonas sp. | |
Phe-Glu | inhibition of methotrexate hydrolysis | Pseudomonas sp. | |
Tyr-Glu | inhibition of methotrexate hydrolysis | Pseudomonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | required | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pseudomonas sp. |
Storage Stability | Organism |
---|---|
-196°C, stable for several months | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
folic acid + H2O | - |
Pseudomonas sp. | pteroic acid + glutamic acid | - |
? | |
methotrexate + H2O | - |
Pseudomonas sp. | 4-amino-4-deoxy-N10-methylpteroic acid + L-glutamate | - |
? | |
additional information | rapid hydrolysis occurs in linkages of the form RCO-Glu and RCO-Gln, whereas those of the form RCO-D-Glu (D-Glu-D-Glu and Z-D-Glu) are not attacked | Pseudomonas sp. | ? | - |
? | |
additional information | no hydrolysis of glutamate or glutamine lacking a free carboxyl alpha-carboxyl | Pseudomonas sp. | ? | - |
? |