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Literature summary for 3.4.17.1 extracted from
- Isolation of Metarhizium anisopliae carboxypeptidase A with native disulfide bonds from the cytosol of Escherichia coli BL21(DE3) (2012), Protein Expr. Purif., 82, 116-124.
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | a strategy is shown for the expression of MeCPA-His6PR in the cytosol of Escherichia coli and a relatively simple procedure to purify it to homogeneity. The bacterial system yields about 0.5 mg of pure enzyme per liter of cell culture and is more convenient and less expensive than is the production of MeCPA in insect cells | Metarhizium anisopliae |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli as a His-tagged fusion protein | Metarhizium anisopliae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Metarhizium anisopliae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using Ni-NTA chromatography, ion exchange chromatography and gel filtration | Metarhizium anisopliae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-(3-[2-furyl]acryloyl)-Phe-Phe + H2O | - |
Metarhizium anisopliae | N-(3-[2-furyl]acryloyl)-Phe + Phe | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| carboxypeptidase a | - |
Metarhizium anisopliae |
| MeCPA | - |
Metarhizium anisopliae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Metarhizium anisopliae |
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Release 2023.1 (January 2023)
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