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Literature summary for 3.4.17.1 extracted from
- Cloning and expression of human colon mast cell carboxypeptidase (2004), World J. Gastroenterol., 10, 342-347.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, expression in Escherichia coli as maltose binding protein-fusion protein with low activity level, methanol-inducible expression in Pichia pastoris with a high activity of the secreted recombinant enzyme | Homo sapiens |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
x * 80000, recombinant fusion enzyme expressed in Escherichia coli, SDS-PAGE, x * 36000, recombinant detagged enzyme expressed in Escherichia coli, SDS-PAGE, x * 37000, recombinant enzyme expressed in Pichia pastoris, SDS-PAGE | Homo sapiens |
| 37000 | - |
x * 80000, recombinant fusion enzyme expressed in Escherichia coli, SDS-PAGE, x * 36000, recombinant detagged enzyme expressed in Escherichia coli, SDS-PAGE, x * 37000, recombinant enzyme expressed in Pichia pastoris, SDS-PAGE | Homo sapiens |
| 80000 | - |
x * 80000, recombinant fusion enzyme expressed in Escherichia coli, SDS-PAGE, x * 36000, recombinant detagged enzyme expressed in Escherichia coli, SDS-PAGE, x * 37000, recombinant enzyme expressed in Pichia pastoris, SDS-PAGE | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant maltose binding protein-fusion enzyme from Escherichia coli by amylose affinity chromatography, digestion with thrombin, and heparin affinity chromatography | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| colon | mast cell | Homo sapiens | - |
| mast cell | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 11.7 | - |
recombinant enzyme secreted from Pichia pastoris, cell-free supernatant | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hippuryl-L-phenylalanine + H2O | - |
Homo sapiens | hippuric acid + L-phenylalanine | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 80000, recombinant fusion enzyme expressed in Escherichia coli, SDS-PAGE, x * 36000, recombinant detagged enzyme expressed in Escherichia coli, SDS-PAGE, x * 37000, recombinant enzyme expressed in Pichia pastoris, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| colon mast cell carboxypeptidase | - |
Homo sapiens |
| MC-CP | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Homo sapiens |
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