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Literature summary for 3.4.17.1 extracted from
- Expression and characterization of human pancreatic preprocarboxypeptidase A1 and preprocarboxypeptidase A2 (1996), Arch. Biochem. Biophys., 332, 8-18.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, expression of the pro-CPA1 in Saccharomyces cerevisiae | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0043 | - |
methotrexate-alpha-phenylalanine | pH 7.4, 25°C, recombinant enzyme | Homo sapiens |  |
| 0.065 | - |
methotrexate-alpha-(1-naphthyl)alanine | pH 7.4, 25°C, recombinant enzyme | Homo sapiens | |
| 0.075 | - |
hippuryl-L-phenylalanine | pH 7.4, 25°C, recombinant enzyme | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
x * 47140, prepro-CPA1, sequence calculation, x * 34000, recombinant mature CPA1, SDS-PAGE, x * 42000, recombinant proCPA1, SDS-PAGE | Homo sapiens |
| 42000 | - |
x * 47140, prepro-CPA1, sequence calculation, x * 34000, recombinant mature CPA1, SDS-PAGE, x * 42000, recombinant proCPA1, SDS-PAGE | Homo sapiens |
| 47140 | - |
x * 47140, prepro-CPA1, sequence calculation, x * 34000, recombinant mature CPA1, SDS-PAGE, x * 42000, recombinant proCPA1, SDS-PAGE | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | the proCPA1 is activated by cleavage by trypsin, the mature enzyme shows 115fold higher activity than the pro-enzyme | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant pro-CPA1 from Saccharomyces cerevisiae by anion exchange and hydrophobic interaction chromatography and another step of anion exchange chromatography followed by dialsis and gel filtration, to homogeneity | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pancreas | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
catalytic efficency of the purified recombinant enzyme | Homo sapiens |
| 7.2 | - |
purified recombinant enzyme, substrate hippuryl-L-phenylalanine | Homo sapiens |
| 850 | - |
purified recombinant enzyme, substrate hippuryl-L-phenyllactate | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hippuryl-DL-phenyllactate + H2O | - |
Homo sapiens | hippuric acid + DL-phenyllactate | - |
? | |
| hippuryl-L-phenylalanine + H2O | - |
Homo sapiens | hippuric acid + L-phenylalanine | - |
? | |
| methotrexate-alpha-(1-naphthyl)alanine + H2O | low activity | Homo sapiens | methotrexate + (1-naphthyl)alanine | - |
? | |
| methotrexate-alpha-phenylalanine + H2O | high activity | Homo sapiens | methotrexate + phenylalanine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 47140, prepro-CPA1, sequence calculation, x * 34000, recombinant mature CPA1, SDS-PAGE, x * 42000, recombinant proCPA1, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CPA1 | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
half-life of purified recombinant CPA2 is 24 min, and 8fold longer than for CPA1 | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.09 | - |
methotrexate-alpha-(1-naphthyl)alanine | pH 7.4, 25°C, recombinant enzyme | Homo sapiens | |
| 1.9 | - |
methotrexate-alpha-phenylalanine | pH 7.4, 25°C, recombinant enzyme | Homo sapiens | |
| 4.3 | - |
hippuryl-L-phenylalanine | pH 7.4, 25°C, recombinant enzyme | Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Homo sapiens |
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