Activating Compound | Comment | Organism | Structure |
---|---|---|---|
chymotrypsin | activates | Squalus acanthias | |
additional information | activation by chymotrypsin and trypsin | Squalus acanthias | |
Trypsin | activates | Squalus acanthias |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Squalus acanthias | |
3-Phenylpropionic acid | Ki: 6.1 mM | Squalus acanthias |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
hippuryl-DL-beta-phenylacetic acid | - |
Squalus acanthias | |
0.21 | - |
carbobenzoxy-Gly-L-Phe | - |
Squalus acanthias | |
0.44 | - |
N-acetyl-Gly-L-phenyllactic acid | - |
Squalus acanthias |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | enhances activation | Squalus acanthias |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
sedimentation equilibrium | Squalus acanthias |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Squalus acanthias | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Squalus acanthias |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pancreas | - |
Squalus acanthias | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.9 | - |
- |
Squalus acanthias |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carbobenzoxy-Gly-L-Phe + H2O | - |
Squalus acanthias | carbobenzoxy-Gly + L-Phe | - |
ir | |
hippuryl-DL-beta-phenylacetic acid + H2O | - |
Squalus acanthias | hippuric acid + DL-phenylacetic acid | - |
? | |
hippuryl-DL-beta-phenyllactic acid + H2O | - |
Squalus acanthias | hippuric acid + 3-phenyllactic acid | - |
ir | |
hippuryl-DL-phenyllactic acid + H2O | - |
Squalus acanthias | hippuric acid + DL-phenyllactic acid | - |
ir | |
N-acetyl-Gly-L-phenyllactic acid + H2O | - |
Squalus acanthias | N-acetyl-Gly + L-phenyllactic acid | - |
ir |
Subunits | Comment | Organism |
---|---|---|
monomer | SDS-PAGE | Squalus acanthias |
More | tendency towards aggregation | Squalus acanthias |
More | amino acid composition described | Squalus acanthias |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Squalus acanthias |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
14.7 | - |
carbobenzoxy-Gly-L-Phe | - |
Squalus acanthias | |
86 | - |
N-acetyl-Gly-L-phenyllactic acid | - |
Squalus acanthias | |
635 | - |
hippuryl-DL-phenyllactic acid | - |
Squalus acanthias |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Squalus acanthias |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
6.1 | - |
3-Phenylpropionic acid | - |
Squalus acanthias |