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Literature summary for 3.4.17.1 extracted from

  • Lacko, A.G.; Neurath, H.
    Studies on procarboxypeptidase A of the spiny pacific dogfish (Squalus acanthias) (1976), Biochemistry, 9, 4680-4690.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
chymotrypsin activates Squalus acanthias
additional information activation by chymotrypsin and trypsin Squalus acanthias
Trypsin activates Squalus acanthias

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Squalus acanthias
3-Phenylpropionic acid Ki: 6.1 mM Squalus acanthias

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
hippuryl-DL-beta-phenylacetic acid
-
Squalus acanthias
0.21
-
carbobenzoxy-Gly-L-Phe
-
Squalus acanthias
0.44
-
N-acetyl-Gly-L-phenyllactic acid
-
Squalus acanthias

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ enhances activation Squalus acanthias

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
sedimentation equilibrium Squalus acanthias

Organism

Organism UniProt Comment Textmining
Squalus acanthias
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Squalus acanthias

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
Squalus acanthias
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.9
-
-
Squalus acanthias

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carbobenzoxy-Gly-L-Phe + H2O
-
Squalus acanthias carbobenzoxy-Gly + L-Phe
-
ir
hippuryl-DL-beta-phenylacetic acid + H2O
-
Squalus acanthias hippuric acid + DL-phenylacetic acid
-
?
hippuryl-DL-beta-phenyllactic acid + H2O
-
Squalus acanthias hippuric acid + 3-phenyllactic acid
-
ir
hippuryl-DL-phenyllactic acid + H2O
-
Squalus acanthias hippuric acid + DL-phenyllactic acid
-
ir
N-acetyl-Gly-L-phenyllactic acid + H2O
-
Squalus acanthias N-acetyl-Gly + L-phenyllactic acid
-
ir

Subunits

Subunits Comment Organism
monomer SDS-PAGE Squalus acanthias
More tendency towards aggregation Squalus acanthias
More amino acid composition described Squalus acanthias

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Squalus acanthias

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
14.7
-
carbobenzoxy-Gly-L-Phe
-
Squalus acanthias
86
-
N-acetyl-Gly-L-phenyllactic acid
-
Squalus acanthias
635
-
hippuryl-DL-phenyllactic acid
-
Squalus acanthias

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Squalus acanthias

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.1
-
3-Phenylpropionic acid
-
Squalus acanthias