BRENDA - Enzyme Database show
show all sequences of 3.4.16.6

Serine-type carboxypeptidase KexA of Aspergillus oryzae has broader substrate specificity than Saccharomyces cerevisiae Kex1 and is required for normal hyphal growth and conidiation

Morita, H.; Tomita, S.; Maeda, H.; Okamoto, A.; Yamagata, Y.; Kusumoto, K.; Amano, H.; Ishida, H.; Takeuchi, M.; Appl. Environ. Microbiol. 78, 8154-8157 (2012)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
a truncated form of KexA (amino acid residues 1 to 526 of KexA), which lack the potential transmembrane region and the succeeding C-terminal sequence (amino acid residues 527 to 625 of KexA) is expressed by using a protein expression system with the pIECS3 vector and Aspergillus nidulans
Aspergillus oryzae
Inhibitors
Inhibitors
Commentary
Organism
Structure
EDTA
14% inhibition
Aspergillus oryzae
monoiodo acetic acid
90% inhibition
Aspergillus oryzae
PMSF
68% inhibition
Aspergillus oryzae
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
58820
-
truncated form, calculated from cDNA
Aspergillus oryzae
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Aspergillus oryzae
-
-
-
Purification (Commentary)
Commentary
Organism
using anion-exchange chromatography and gel filtration
Aspergillus oryzae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
angiotensin 1 + H2O
truncated KexA releases amino acid residues from the C terminus of angiotensin I
731176
Aspergillus oryzae
?
-
-
-
?
benzoyl-Gly-Arg + H2O
enzyme shows very low activity
731176
Aspergillus oryzae
benzoyl-Gly + L-arginine
-
-
-
?
benzyloxycarbonyl-Gly-Lys + H2O
enzyme shows very low activity
731176
Aspergillus oryzae
benzyloxycarbonyl-Gly + L-lysine
-
-
-
?
benzyloxycarbonyl-Phe-Leu + H2O
enzyme shows very low activity
731176
Aspergillus oryzae
benzyloxycarbonyl-Phe + L-leucine
-
-
-
?
benzyloxycarbonyl-Phe-Tyr-Leu + H2O
-
731176
Aspergillus oryzae
benzyloxycarbonyl-Phe-Tyr + L-leucine
-
-
-
?
benzyloxycarbonyl-Tyr-Leu + H2O
enzyme shows very low activity
731176
Aspergillus oryzae
benzyloxycarbonyl-Tyr + L-leucine
-
-
-
?
bradykinin I + H2O
truncated KexA releases amino acid residues from the C terminus of bradykinin I
731176
Aspergillus oryzae
?
-
-
-
?
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
40
-
maximum temperature at which the enzyme has residual activity of over 60% relative to maximum activity
Aspergillus oryzae
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
-
-
Aspergillus oryzae
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
5
8
-
Aspergillus oryzae
Cloned(Commentary) (protein specific)
Commentary
Organism
a truncated form of KexA (amino acid residues 1 to 526 of KexA), which lack the potential transmembrane region and the succeeding C-terminal sequence (amino acid residues 527 to 625 of KexA) is expressed by using a protein expression system with the pIECS3 vector and Aspergillus nidulans
Aspergillus oryzae
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
EDTA
14% inhibition
Aspergillus oryzae
monoiodo acetic acid
90% inhibition
Aspergillus oryzae
PMSF
68% inhibition
Aspergillus oryzae
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
58820
-
truncated form, calculated from cDNA
Aspergillus oryzae
Purification (Commentary) (protein specific)
Commentary
Organism
using anion-exchange chromatography and gel filtration
Aspergillus oryzae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
angiotensin 1 + H2O
truncated KexA releases amino acid residues from the C terminus of angiotensin I
731176
Aspergillus oryzae
?
-
-
-
?
benzoyl-Gly-Arg + H2O
enzyme shows very low activity
731176
Aspergillus oryzae
benzoyl-Gly + L-arginine
-
-
-
?
benzyloxycarbonyl-Gly-Lys + H2O
enzyme shows very low activity
731176
Aspergillus oryzae
benzyloxycarbonyl-Gly + L-lysine
-
-
-
?
benzyloxycarbonyl-Phe-Leu + H2O
enzyme shows very low activity
731176
Aspergillus oryzae
benzyloxycarbonyl-Phe + L-leucine
-
-
-
?
benzyloxycarbonyl-Phe-Tyr-Leu + H2O
-
731176
Aspergillus oryzae
benzyloxycarbonyl-Phe-Tyr + L-leucine
-
-
-
?
benzyloxycarbonyl-Tyr-Leu + H2O
enzyme shows very low activity
731176
Aspergillus oryzae
benzyloxycarbonyl-Tyr + L-leucine
-
-
-
?
bradykinin I + H2O
truncated KexA releases amino acid residues from the C terminus of bradykinin I
731176
Aspergillus oryzae
?
-
-
-
?
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
40
-
maximum temperature at which the enzyme has residual activity of over 60% relative to maximum activity
Aspergillus oryzae
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
-
-
Aspergillus oryzae
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
5
8
-
Aspergillus oryzae
General Information
General Information
Commentary
Organism
malfunction
kexA deletion strain shows slightly lower growth speed. Deletion strain exhibits scarcely branched hyphae, whereas an kexA-overexpressing strain exhibits a hyperbranching phenotype
Aspergillus oryzae
General Information (protein specific)
General Information
Commentary
Organism
malfunction
kexA deletion strain shows slightly lower growth speed. Deletion strain exhibits scarcely branched hyphae, whereas an kexA-overexpressing strain exhibits a hyperbranching phenotype
Aspergillus oryzae
Other publictions for EC 3.4.16.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
732160
Garcia-Pardo
Amyloid formation by human car ...
Homo sapiens
J. Biol. Chem.
289
33783-33796
2014
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1
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731593
Jin
SiRNA-targeted carboxypeptidas ...
Homo sapiens
Cell Biol. Int.
37
929-939
2013
-
-
-
-
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-
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3
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4
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1
1
1
1
-
-
731598
Carmona-Gutierrez
The cell death protease Kex1p ...
Saccharomyces cerevisiae
Cell Cycle
12
1704-1712
2013
-
-
-
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-
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-
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2
2
-
-
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731176
Morita
Serine-type carboxypeptidase K ...
Aspergillus oryzae
Appl. Environ. Microbiol.
78
8154-8157
2012
-
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1
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3
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1
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3
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7
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7
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1
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1
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1
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1
1
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732850
Thomas
Testosterone and prolactin inc ...
Homo sapiens
Prostate
72
450-460
2012
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1
2
2
1
-
-
698771
Hauptmann
Kex1 protease is involved in y ...
Saccharomyces cerevisiae
J. Biol. Chem.
283
19151-19163
2008
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1
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1
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701056
Pozzuolo
Efficient bacterial expression ...
Kluyveromyces lactis
Protein Expr. Purif.
59
334-341
2008
-
1
1
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1
2
1
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4
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1
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1
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683223
Serviene
-
Influence of Kex1p and Kex2p p ...
Saccharomyces cerevisiae
Biologija (Vilnius)
53
35-38
2007
-
-
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-
1
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1
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683695
Heiman
The Golgi-resident protease Ke ...
Saccharomyces cerevisiae
J. Cell Biol.
176
209-222
2007
-
1
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1
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3
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669448
Sidyelyeva
Characterization of the molecu ...
Drosophila melanogaster
J. Biol. Chem.
281
13844-13852
2006
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1
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4
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669602
Kalinina
Drosophila S2 cells produce mu ...
Drosophila melanogaster
J. Cell. Biochem.
99
770-783
2006
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2
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5
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667510
O'Malley
Characterization of a novel, c ...
Homo sapiens, Rattus norvegicus
Biochem. J.
390
665-673
2005
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4
2
2
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6
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1
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1
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3
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665316
Satoh
Microbial serine carboxypeptid ...
Triticum aestivum
J. Antibiot.
57
316-325
2004
-
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4
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4
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647241
Sidyelyeva
Characterization of Drosophila ...
Drosophila melanogaster
J. Biol. Chem.
277
49613-49620
2002
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1
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2
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4
2
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4
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1
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3
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3
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2
3
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647233
Shilton
Crystal structure of Kex1delta ...
Saccharomyces cerevisiae
Biochemistry
36
9002-9012
1997
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1
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647236
Shilton
Crystallization of a soluble f ...
Saccharomyces cerevisiae
Protein Sci.
5
395-397
1996
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647192
Remington
Carboxypeptidases C and D ...
Saccharomyces cerevisiae, Triticum aestivum
Methods Enzymol.
244
231-248
1994
-
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2
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2
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647234
Latchinian-Sadek
Secretion, purification and ch ...
Saccharomyces cerevisiae
Eur. J. Biochem.
219
647-652
1994
-
-
-
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4
1
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1
1
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1
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1
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2
1
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-
1
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1
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4
-
1
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1
1
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1
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2
1
-
-
-
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1
-
-
1
-
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-
-
-
-
647238
Latchinian-Sadek
Expression, purification, and ...
Saccharomyces cerevisiae
J. Biol. Chem.
268
534-540
1993
-
-
-
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-
7
3
-
2
-
1
-
2
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-
1
-
-
-
1
-
9
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
7
-
3
-
2
-
1
-
-
-
1
-
-
1
-
9
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
647231
Liao
Refined atomic model of wheat ...
Triticum aestivum
Biochemistry
31
9796-9812
1992
-
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1
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1
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1
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1
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647235
Thomas
Yeast KEX1 protease cleaves a ...
Saccharomyces cerevisiae
J. Biol. Chem.
265
10821-10824
1990
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647232
Dmochowska
Yeast KEX1 gene encodes a puta ...
Saccharomyces cerevisiae
Cell
50
573-584
1987
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