BRENDA - Enzyme Database show
show all sequences of 3.4.16.6

Efficient bacterial expression of fusion proteins and their selective processing by a recombinant Kex-1 protease

Pozzuolo, S.; Breme, U.; Salis, B.; Taylor, G.; Tonon, G.; Orsini, G.; Protein Expr. Purif. 59, 334-341 (2008)

Data extracted from this reference:

Application
Application
Commentary
Organism
biotechnology
Kex-1-C611 is proposed as a convenient biotechnological reagent for the cleavage of fusion proteins
Kluyveromyces lactis
Cloned(Commentary)
Commentary
Organism
a truncated form of Kex-1, Kex-1-C611, is constructed comprising the amino acid residues from 104-611 and lacks the transmembrane domain
Kluyveromyces lactis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
-
Kluyveromyces lactis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
58000
-
calculated, truncated Kex-1-C611
Kluyveromyces lactis
67000
-
determined by SDS-PAGE, truncated Kex-1-C611
Kluyveromyces lactis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Kluyveromyces lactis
preferential release of a C-terminal arginine or lysine residue
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Kluyveromyces lactis
-
-
-
Purification (Commentary)
Commentary
Organism
archieved by a two-step chromatographic process using cation-exchange, SP Sepharose, followed by anion-exchange, DEAE Sepharose FF resin
Kluyveromyces lactis
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
60 U/ml, purified truncated Kex-1-C611
Kluyveromyces lactis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
preferential release of a C-terminal arginine or lysine residue
701056
Kluyveromyces lactis
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
cleavage assay
Kluyveromyces lactis
Application (protein specific)
Application
Commentary
Organism
biotechnology
Kex-1-C611 is proposed as a convenient biotechnological reagent for the cleavage of fusion proteins
Kluyveromyces lactis
Cloned(Commentary) (protein specific)
Commentary
Organism
a truncated form of Kex-1, Kex-1-C611, is constructed comprising the amino acid residues from 104-611 and lacks the transmembrane domain
Kluyveromyces lactis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
-
Kluyveromyces lactis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
58000
-
calculated, truncated Kex-1-C611
Kluyveromyces lactis
67000
-
determined by SDS-PAGE, truncated Kex-1-C611
Kluyveromyces lactis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Kluyveromyces lactis
preferential release of a C-terminal arginine or lysine residue
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
archieved by a two-step chromatographic process using cation-exchange, SP Sepharose, followed by anion-exchange, DEAE Sepharose FF resin
Kluyveromyces lactis
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
60 U/ml, purified truncated Kex-1-C611
Kluyveromyces lactis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
preferential release of a C-terminal arginine or lysine residue
701056
Kluyveromyces lactis
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
cleavage assay
Kluyveromyces lactis
Other publictions for EC 3.4.16.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
732160
Garcia-Pardo
Amyloid formation by human car ...
Homo sapiens
J. Biol. Chem.
289
33783-33796
2014
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731593
Jin
SiRNA-targeted carboxypeptidas ...
Homo sapiens
Cell Biol. Int.
37
929-939
2013
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1
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731598
Carmona-Gutierrez
The cell death protease Kex1p ...
Saccharomyces cerevisiae
Cell Cycle
12
1704-1712
2013
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2
2
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731176
Morita
Serine-type carboxypeptidase K ...
Aspergillus oryzae
Appl. Environ. Microbiol.
78
8154-8157
2012
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7
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732850
Thomas
Testosterone and prolactin inc ...
Homo sapiens
Prostate
72
450-460
2012
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1
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698771
Hauptmann
Kex1 protease is involved in y ...
Saccharomyces cerevisiae
J. Biol. Chem.
283
19151-19163
2008
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701056
Pozzuolo
Efficient bacterial expression ...
Kluyveromyces lactis
Protein Expr. Purif.
59
334-341
2008
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683223
Serviene
-
Influence of Kex1p and Kex2p p ...
Saccharomyces cerevisiae
Biologija (Vilnius)
53
35-38
2007
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1
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683695
Heiman
The Golgi-resident protease Ke ...
Saccharomyces cerevisiae
J. Cell Biol.
176
209-222
2007
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669448
Sidyelyeva
Characterization of the molecu ...
Drosophila melanogaster
J. Biol. Chem.
281
13844-13852
2006
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669602
Kalinina
Drosophila S2 cells produce mu ...
Drosophila melanogaster
J. Cell. Biochem.
99
770-783
2006
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667510
O'Malley
Characterization of a novel, c ...
Homo sapiens, Rattus norvegicus
Biochem. J.
390
665-673
2005
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665316
Satoh
Microbial serine carboxypeptid ...
Triticum aestivum
J. Antibiot.
57
316-325
2004
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647241
Sidyelyeva
Characterization of Drosophila ...
Drosophila melanogaster
J. Biol. Chem.
277
49613-49620
2002
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2
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3
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647233
Shilton
Crystal structure of Kex1delta ...
Saccharomyces cerevisiae
Biochemistry
36
9002-9012
1997
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647236
Shilton
Crystallization of a soluble f ...
Saccharomyces cerevisiae
Protein Sci.
5
395-397
1996
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647192
Remington
Carboxypeptidases C and D ...
Saccharomyces cerevisiae, Triticum aestivum
Methods Enzymol.
244
231-248
1994
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647234
Latchinian-Sadek
Secretion, purification and ch ...
Saccharomyces cerevisiae
Eur. J. Biochem.
219
647-652
1994
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647238
Latchinian-Sadek
Expression, purification, and ...
Saccharomyces cerevisiae
J. Biol. Chem.
268
534-540
1993
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9
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647231
Liao
Refined atomic model of wheat ...
Triticum aestivum
Biochemistry
31
9796-9812
1992
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647235
Thomas
Yeast KEX1 protease cleaves a ...
Saccharomyces cerevisiae
J. Biol. Chem.
265
10821-10824
1990
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4
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647232
Dmochowska
Yeast KEX1 gene encodes a puta ...
Saccharomyces cerevisiae
Cell
50
573-584
1987
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