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Literature summary for 3.4.16.5 extracted from

  • Yu, X.; Zhai, C.; Zhong, X.; Tang, W.; Wang, X.; Yang, H.; Chen, W.; Ma, L.
    High-level expression and characterization of carboxypeptidase Y from Saccharomyces cerevisiae in Pichia pastoris GS115 (2015), Biotechnol. Lett., 37, 161-167 .
    View publication on PubMed
Search for more literature for 3.4.16.5

Application

Application Comment Organism
synthesis synthesis of CPY in Pichia pastoris as procarboxypeptidase Y with a yield of about 605 mg/l in shake-flasks after 168 h induction with 1 % (v/v) methanol. This precursor is cleaved by endogenous proteinases of Pichia pastoris and released into the fermentation broth as active carboxypeptidase Y within 2 weeks at 10°C. The recombinant enzyme is optimally active at 30°C and pH 6.0, with an optimal activity of about 305 U/mg Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P00729
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
305
-
 recombinant enzyme, pH 6.0, 30°C Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-L-phenylalanyl-L-leucine + H2O
-
 Saccharomyces cerevisiae benzyloxycarbonyl-Phe + Leu
-
 ?

Synonyms

Synonyms Comment Organism
CPY
-
 Saccharomyces cerevisiae
PRC1
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 recombinant enzyme Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 recombinant enzyme Saccharomyces cerevisiae