General Stability | Organism |
---|---|
urea, 6 M, about 80% of activity is retained after 1 h | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | - |
Saccharomyces cerevisiae | |
Ba2+ | partial | Saccharomyces cerevisiae | |
benzyloxycarbonyl-L-phenylalanine chloromethyl ketone | - |
Saccharomyces cerevisiae | |
Ca2+ | partial | Saccharomyces cerevisiae | |
Co2+ | partial | Saccharomyces cerevisiae | |
Cr2+ | partial | Saccharomyces cerevisiae | |
Cu+ | partial | Saccharomyces cerevisiae | |
diisopropyl fluorophosphate | - |
Saccharomyces cerevisiae | |
Fe2+ | partial | Saccharomyces cerevisiae | |
Fe3+ | partial | Saccharomyces cerevisiae | |
Hg2+ | - |
Saccharomyces cerevisiae | |
L-amino acids | competitive | Saccharomyces cerevisiae | |
Mg2+ | partial | Saccharomyces cerevisiae | |
Mn2+ | partial | Saccharomyces cerevisiae | |
additional information | no inhibition by 1,10-phenanthroline; not inhibition by EDTA | Saccharomyces cerevisiae | |
NH2-blocked amino acids | - |
Saccharomyces cerevisiae | |
Ni2+ | partial | Saccharomyces cerevisiae | |
p-chloromercuribenzenesulfonate | - |
Saccharomyces cerevisiae | |
p-hydroxymercuribenzoate | - |
Saccharomyces cerevisiae | |
phenylmethane sulfonylfluoride | - |
Saccharomyces cerevisiae | |
Product analogs | - |
Saccharomyces cerevisiae | |
substrate analogs | - |
Saccharomyces cerevisiae | |
trans-cinnamate | - |
Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
endothelin | - |
Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
61000 | - |
- |
Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Saccharomyces cerevisiae | - |
? | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
2-chloroethanol | 10% stable at pH 7.0 for at least 15 min | Saccharomyces cerevisiae |
dioxane | 30% stable at pH 7.0 for at least 15 min | Saccharomyces cerevisiae |
Ethylene glycol | 60% stable at pH 7.0 for at least 15 min | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
baker's yeast | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 16 residues of glucosamine and about 15% hexose | Saccharomyces cerevisiae |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-Tyr-ethyl ester + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
benzyloxycarbonyl-Phe-Leu + H2O | - |
Saccharomyces cerevisiae | benzyloxycarbonyl-Phe + Leu | - |
? | |
endothelin + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
endothelin I + H2O | containing the hydrophobic sequence Ile19-Ile20-Trp21-OH | Saccharomyces cerevisiae | endothelin(1-20) + Trp | - |
? | |
additional information | - |
Saccharomyces cerevisiae | ? | - |
? | |
additional information | no hydrolysis of poly-L-lysine | Saccharomyces cerevisiae | ? | - |
? | |
additional information | no hydrolysis of poly-L-proline | Saccharomyces cerevisiae | ? | - |
? | |
pancreatic carboxypeptidase A + H2O | - |
Saccharomyces cerevisiae | hydrolyzed carboxypeptidase A + C-terminal amino acid | - |
? | |
pancreatic carboxypeptidase B + H2O | - |
Saccharomyces cerevisiae | hydrolyzed pancreatic carboxypeptidase B + C-terminal amino acid | - |
? | |
poly-alpha-L-aspartic acid + H2O | slowly | Saccharomyces cerevisiae | ? | - |
? | |
poly-alpha-L-glutamic acid + H2O | - |
Saccharomyces cerevisiae | glutamic acid | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
pH 5.5-8, stable | Saccharomyces cerevisiae |
60 | - |
quick loss of activity above | Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
24.2 | - |
endothelin | - |
Saccharomyces cerevisiae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | - |
quick loss of activity below | Saccharomyces cerevisiae |
5.5 | 8 | 8 h, 25°C, stable | Saccharomyces cerevisiae |
7 | - |
at temperatures above 25°C the enzyme is most stable at pH 7.0 | Saccharomyces cerevisiae |