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Literature summary for 3.4.16.4 extracted from

  • Binda, E.; Marcone, G.; Berini, F.; Pollegioni, L.; Marinelli, F.
    Streptomyces spp. as efficient expression system for a D,D-peptidase/D,D-carboxypeptidase involved in glycopeptide antibiotic resistance (2013), BMC Biotechnol., 13, 24.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.16.4

Application

Application Comment Organism
synthesis highest yield of protein expression and purification from 12 mg of protein per liter of culture at 3 l bioreactor-scale is achieved in Streptomyces venezuelae ATCC 10595, a fast growing Streptomyces susceptible to glycopeptides. The addition of His6-tag at the N-terminus of the protein abolishes its biological activity either in vitro or in vivo assays. The addition of His6-tag at the C-terminus of the protein leads to a functional protein Nonomuraea sp.

Cloned(Commentary)

Cloned (Comment) Organism
expression in Streptomyces spp. Nonomuraea sp.

Protein Variants

Protein Variants Comment Organism
additional information the addition of His6-tag at the N-terminus of the protein abolishes its biological activity either in vitro or in vivo assays. The addition of His6-tag at the C-terminus of the protein leads to a functional protein Nonomuraea sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
cell wall enzyme preferentially accumulates in the cell wall fractions obtained by the step of spheroplast preparation Nonomuraea sp. 5618
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22100
-
 x * 25000, SDS-PAGE of His-tagged protein, x * 22100, calculated for native protein Nonomuraea sp.
25000
-
 x * 25000, SDS-PAGE of His-tagged protein, x * 22100, calculated for native protein Nonomuraea sp.

Organism

Organism UniProt Comment Textmining
Nonomuraea sp. Q7WZ84
-
-
Nonomuraea sp. ATCC 39727 Q7WZ84
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3 8 substrate Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala , pH 7.4, 25°C Nonomuraea sp.
18
-
 substrate D-Ala-D-Ala, pH 7.4, 25°C Nonomuraea sp.
40
-
 substrate acetyl-L-Lys-D-Ala-D-Ala, pH 7.4, 25°C Nonomuraea sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-L-Lys-D-Ala-D-Ala + H2O
-
 Nonomuraea sp. acetyl-L-Lys-D-Ala + D-Ala
-
 ?
acetyl-L-Lys-D-Ala-D-Ala + H2O
-
 Nonomuraea sp. ATCC 39727 acetyl-L-Lys-D-Ala + D-Ala
-
 ?
D-Ala-D-Ala + H2O
-
 Nonomuraea sp. D-Ala + D-Ala
-
 ?
D-Ala-D-Ala + H2O
-
 Nonomuraea sp. ATCC 39727 D-Ala + D-Ala
-
 ?
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + H2O
-
 Nonomuraea sp. Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala + D-Ala
-
 ?
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + H2O
-
 Nonomuraea sp. ATCC 39727 Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala + D-Ala
-
 ?

Subunits

Subunits Comment Organism
? x * 25000, SDS-PAGE of His-tagged protein, x * 22100, calculated for native protein Nonomuraea sp.

Synonyms

Synonyms Comment Organism
Dbv7
-
 Nonomuraea sp.
VanYn
-
 Nonomuraea sp.

General Information

General Information Comment Organism
physiological function enzyme has a role in removing the last D-Ala from the pentapeptide peptidoglycan precursors and reprogramming cell wall biosynthesis. Isoform C-His6-VanYn over-expression confers glycopeptide resistance to Streptomyces venezuelae. The addition of His6-tag at the N-terminus of the protein abolishes its biological activity either in vitro or in vivo assays Nonomuraea sp.