Crystallization (Comment) | Organism |
---|---|
purified Pbp1b in complex with N-benzoyl-D-Ala or pseudo-substrate N-benzoyl-D-alanylmercaptoacetic acid thioester, hanging drop vapor diffusion method, mixing of 0.001 ml of 6-10 mg/ml protein and 5 mM N-benzoyl-D-alanylmercaptoacetic acid thioester, with 0.001 ml of well solution containing 50 mM HEPES, pH 7.0, 0.8 M (NH4)2SO4, 2.8 M NaCl at 15°C, X-ray difraction structure determination and analysis at 2.5 A resolution, molecular replacement | Streptococcus pneumoniae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
nitrocefin | - |
Streptococcus pneumoniae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Streptococcus pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Streptococcus pneumoniae | low molecular mass PBPs can exert carboxypeptidase and/or endopeptidase activities and play auxiliary roles in the peptidoglycan metabolism process | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pneumoniae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | low molecular mass PBPs can exert carboxypeptidase and/or endopeptidase activities and play auxiliary roles in the peptidoglycan metabolism process | Streptococcus pneumoniae | ? | - |
? | |
additional information | the serine protease catalyzes glycosyl transfer, i.e. polymerization of glycan chains, and transpeptidation, i.e. cross-linking of stem peptides, which are essential for peptidoglycan stability in bacterial cell wall synthesis, and for the cell division process, overview | Streptococcus pneumoniae | ? | - |
? | |
N-acetyl-L-Lys-D-Ala-D-Ala + H2O | - |
Streptococcus pneumoniae | N-acetyl-L-Lys-D-Ala + D-Ala | - |
? | |
N-benzoyl-D-Ala + H2O | substrate binding structure | Streptococcus pneumoniae | benzoate + D-Ala | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | PBP1b structure analysis, overview | Streptococcus pneumoniae |
Synonyms | Comment | Organism |
---|---|---|
PBP | - |
Streptococcus pneumoniae |
PBP1b | - |
Streptococcus pneumoniae |
penicillin-binding protein | - |
Streptococcus pneumoniae |