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Literature summary for 3.4.16.4 extracted from

  • Van der Linden, M.P.G.; Mottl, H.; Keck, W.
    Cytoplasmic high-level expression of a soluble, enzymatically active form of the Escherichia coli penicillin-binding protein 5 and purification by dye chromatography (1992), Eur. J. Biochem., 204, 197-202.
    View publication on PubMed
Search for more literature for 3.4.16.4

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of truncated enzyme Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information truncated forms of the enzyme Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
14
-
 Nalpha,Nepsilon-Diacetyl-L-Lys-D-Ala-D-Ala
-
 Escherichia coli
14
-
 Nalpha,Nepsilon-Diacetyl-L-Lys-D-Ala-D-Ala
-
 Streptomyces sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Escherichia coli
-
-
extracellular
-
 Streptomyces sp.
-
-
membrane
-
 Escherichia coli 16020
-
membrane
-
 Streptomyces sp. 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetyl-muramyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanyl-D-alanine Streptomyces sp.
-
 ?
-
 ?
UDP-N-acetyl-muramyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanyl-D-alanine Streptomyces sp. R39
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Streptomyces sp.
-
 R39
-
Streptomyces sp. R39
-
 R39
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + H2O
-
 Escherichia coli Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala + D-alanine
-
 ?
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + H2O
-
 Streptomyces sp. Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala + D-alanine
-
 ?
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + H2O
-
 Streptomyces sp. R39 Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala + D-alanine
-
 ?
UDP-N-acetyl-muramyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanyl-D-alanine
-
 Streptomyces sp. ?
-
 ?
UDP-N-acetyl-muramyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanyl-D-alanine
-
 Streptomyces sp. R39 ?
-
 ?
UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanyl-D-alanine + H2O
-
 Streptomyces sp. UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanine + D-alanine
-
 ?
UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanyl-D-alanine + H2O
-
 Streptomyces sp. R39 UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanine + D-alanine
-
 ?