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Literature summary for 3.4.16.2 extracted from

  • Lyons, P.J.; Callaway, M.B.; Fricker, L.D.
    Characterization of carboxypeptidase a6, an extracellular matrix peptidase (2008), J. Biol. Chem., 283, 7054-7063.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
a cDNA fragment encoding human pro-CPA6 is subcloned into the pcDNA3.1 vector Homo sapiens
expressed in AT-20 cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Benzylsuccinic acid
-
Homo sapiens
potato carboxypeptidase inhibitor
-
Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the 37-kDa active form of the protein is secreted into the extracellular matrix Homo sapiens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
active form, determined by SDS-PAGE and Western blot analysis Homo sapiens
50000
-
pro-CPA6, determined by SDS-PAGE and Western blot analysis Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Homo sapiens
-
embryo embryonic tissue Homo sapiens
-
HEK-293T cell used for transfection Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-(2-furyl)acryloyl-peptide + H2O substrate used in the carboxypeptidase assay Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
carboxypeptidase A6
-
Homo sapiens
CPA6
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
carboxypeptidase assay Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
carboxypeptidase assay Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
7 9
-
Homo sapiens

pI Value

Organism Comment pI Value Maximum pI Value
Homo sapiens predicted
-
9.9

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00005
-
-
Homo sapiens potato carboxypeptidase inhibitor
0.007
-
-
Homo sapiens Benzylsuccinic acid