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Literature summary for 3.4.16.2 extracted from
- Recombinant prolylcarboxypeptidase activates plasma prekallikrein (2004), Blood, 15, 4554-4561.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cDNA cloned in pMT/BIP/V5-HIS-C, transfected into Schneider insect cells | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| angiotensin II | IC50: 3 mM, inhibits hydrolysis of Gly-Pro-4-nitroanilide | Homo sapiens |  |
| anti-PRCP | - |
Homo sapiens | |
| benzyloxycarbonyl-Pro-Pro-aldehyde-dimethyl acetal | - |
Homo sapiens | |
| benzyloxycarbonyl-Pro-prolinal | IC50: 0.5 mM, inhibits hydrolysis of H-Gly-Pro-4-nitroanilide | Homo sapiens | |
| bradykinin | - |
Homo sapiens | |
| corn trypsin inhibitor | binds to prekallikrein to prevent activation, but does not directly inhibit the active site of either enzyme | Homo sapiens | |
| diisopropyl fluorophosphonate | IC50: 50 nM | Homo sapiens | |
| leupeptin | IC50: 0.8 mM, inhibits hydrolysis of Gly-Pro-4-nitroanilide | Homo sapiens | |
| additional information | no inhibition by 1 mM EDTA, bradykinin 1-5 or angiotensin 1-7 | Homo sapiens | |
| phenylmethylsulfonyl fluoride | IC50: 0.6 mM | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000017 | - |
prekallikrein | - |
Homo sapiens | |
| 2.4 | - |
Ala-Pro-4-nitroanilide | pH 5.5, 37°C | Homo sapiens | |
| 4 | - |
Gly-Pro-4-nitroanilide | pH 5.5, 37°C | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Homo sapiens | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | optimal activity at 0.01 mM | Homo sapiens | |
| Mg2+ | optimal activity at 1 mM | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| umbilical vein endothelial cell line | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Ala-Pro-4-nitroanilide + H2O | - |
Homo sapiens | Ala-Pro + 4-nitroaniline | - |
? | |
| Gly-Pro-4-nitroanilide + H2O | - |
Homo sapiens | Gly-Pro + 4-nitroaniline | - |
? | |
| prekallikrein + H2O | - |
Homo sapiens | additional information | production of a 51000 Da heavy chain and 2 faint light chains of 39000 Da and 36000 Da | ? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PRCP | - |
Homo sapiens |
| serine protease prolylcarboxypeptidase | - |
Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.1 | - |
- |
Homo sapiens |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.00005 | - |
IC50: 50 nM | Homo sapiens | diisopropyl fluorophosphonate | |
| 0.5 | - |
IC50: 0.5 mM, inhibits hydrolysis of H-Gly-Pro-4-nitroanilide | Homo sapiens | benzyloxycarbonyl-Pro-prolinal | |
| 0.6 | - |
IC50: 0.6 mM | Homo sapiens | phenylmethylsulfonyl fluoride | |
| 0.8 | - |
IC50: 0.8 mM, inhibits hydrolysis of Gly-Pro-4-nitroanilide | Homo sapiens | leupeptin | |
| 3 | - |
IC50: 3 mM, inhibits hydrolysis of Gly-Pro-4-nitroanilide | Homo sapiens | angiotensin II | |
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