Application | Comment | Organism |
---|---|---|
food industry | the production of beta-dipeptides from cyanophycin by cyanophycinase is economically important, because di- and tripeptides are more efficiently utilized than intact proteins or free amino acids, have a greater nutritional value, and are better absorbed | Pseudomonas alcaligenes |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-(2-aminoethyl) benzenesulfonylfluoride | cyanophycinase is strongly inhibited | Pseudomonas alcaligenes | |
additional information | no inhibition by EDTA; no inhibition by leupeptin | Pseudomonas alcaligenes | |
N-bromosuccinimide | total inhibition | Pseudomonas alcaligenes | |
phenylmethylsulfonyl fluoride | cyanophycinase is strongly inhibited | Pseudomonas alcaligenes |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Pseudomonas alcaligenes | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
SDS-PAGE | Pseudomonas alcaligenes |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[L-Asp(4-Arg)]n + H2O | Pseudomonas alcaligenes | - |
[L-Asp(4-Arg)]n-1 + [L-Asp(4-Arg)] | - |
? | |
[L-Asp(4-Arg)]n + H2O | Pseudomonas alcaligenes DIP1 | - |
[L-Asp(4-Arg)]n-1 + [L-Asp(4-Arg)] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas alcaligenes | - |
- |
- |
Pseudomonas alcaligenes DIP1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
centrifugation, proteins are concentrated and desalted using a cross-flow polyethersulfone cassette with a cutoff point of 30 kDa, a purification method for cyanophycinase is developed which depended on the strong affinity of the enzyme in the crude extract for its insoluble substrate cyanophycin | Pseudomonas alcaligenes |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture filtrate | - |
Pseudomonas alcaligenes | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
[L-Asp(4-Arg)]n + H2O | - |
Pseudomonas alcaligenes | [L-Asp(4-Arg)]n-1 + [L-Asp(4-Arg)] | - |
? | |
[L-Asp(4-Arg)]n + H2O | 2 g/l citrate, pH 6.5, and 37°C are ideal parameters for cyanophycinase production | Pseudomonas alcaligenes | [L-Asp(4-Arg)]n-1 + [L-Asp(4-Arg)] | - |
? | |
[L-Asp(4-Arg)]n + H2O | - |
Pseudomonas alcaligenes DIP1 | [L-Asp(4-Arg)]n-1 + [L-Asp(4-Arg)] | - |
? | |
[L-Asp(4-Arg)]n + H2O | 2 g/l citrate, pH 6.5, and 37°C are ideal parameters for cyanophycinase production | Pseudomonas alcaligenes DIP1 | [L-Asp(4-Arg)]n-1 + [L-Asp(4-Arg)] | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CGPase | - |
Pseudomonas alcaligenes |
CphEal | - |
Pseudomonas alcaligenes |
cyanophycinase | - |
Pseudomonas alcaligenes |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Pseudomonas alcaligenes |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 80 | 20 min, sodium phosphate buffer pH 7.0 | Pseudomonas alcaligenes |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
maximum degradation activity at 50°C and complete inactivation at 68°C | Pseudomonas alcaligenes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Pseudomonas alcaligenes |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | 20 min, 30°C | Pseudomonas alcaligenes |