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Literature summary for 3.4.15.6 extracted from

  • Richter, R.; Hejazi, M.; Kraft, R.; Ziegler, K.; Lockau, W.
    Cyanophycinase, a peptidase degrading the cyanobacterial reserve material multi-L-arginyl-poly-aspartic acid (cyanophycin). Molecular cloning of the gene of Synechocystis sp. PCC 6803, expression in Escherichia coli, and biochemical characterization of the purified enzyme (1999), Eur. J. Biochem., 263, 163-169.
    View publication on PubMed
Search for more literature for 3.4.15.6

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli DH5alpha Synechocystis sp.

Inhibitors

Inhibitors Comment Organism Structure
3,4-dichloro-isocoumarin 78% inhibition at 1 mM, 56% inhibition at 0.1 mM Synechocystis sp.
additional information not inhibitory: pepstatin, diazoacetyl-DL-nor-leucine-methyl ester, aprotinin Synechocystis sp.
pefabloc i.e. 4-[2-aminoethyl]benzenesulfonyl fluoride, 79% inhibition at 20 mM, 23% inhibition at 8 mM Synechocystis sp.
phenylmethanesulfonyl fluoride 45% inhibition at 10 mM, 8% inhibition at 1 mM Synechocystis sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
 1 * 27000, SDS-PAGE Synechocystis sp.
28000
-
 gel filtration Synechocystis sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[L-Asp(4-Arg)]n + H2O Synechocystis sp.
-
 [L-Asp(4-Arg)]n-1 + [L-Asp(4-Arg)]
-
 ?

Organism

Organism UniProt Comment Textmining
Synechocystis sp. P73832 strain PCC 6803
-

Purification (Commentary)

Purification (Comment) Organism
-
 Synechocystis sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[L-Asp(4-Arg)]n + H2O
-
 Synechocystis sp. [L-Asp(4-Arg)]n-1 + [L-Asp(4-Arg)]
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 27000, SDS-PAGE Synechocystis sp.

Synonyms

Synonyms Comment Organism
CphB
-
 Synechocystis sp.
cyanophycinase
-
 Synechocystis sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Synechocystis sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 broad pH maximum between pH 7 and pH 8, peaking at pH 7.5 Synechocystis sp.

pH Range

pH Minimum pH Maximum Comment Organism
6
-
 30% of maximal activity Synechocystis sp.

pI Value

Organism Comment pI Value Maximum pI Value
Synechocystis sp. calculated from sequence
-
 6.51