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Literature summary for 3.4.15.5 extracted from

  • Cunha, C.E.; Magliarelli, H.d.e..F.; Paschoalin, T.; Nchinda, A.T.; Lima, J.C.; Juliano, M.A.; Paiva, P.B.; Sturrock, E.D.; Travassos, L.R.; Carmona, A.K.
    Catalytic properties of recombinant dipeptidyl carboxypeptidase from Escherichia coli: a comparative study with angiotensin I-converting enzyme (2009), Biol. Chem., 390, 931-940.
    View publication on PubMed
Search for more literature for 3.4.15.5

Cloned(Commentary)

Cloned (Comment) Organism
phylogenetic analysis, expression of His-tagged Dcp in Escherichia coli strain BL21(DE3) Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
(5S)-5-[(N-benzoyl)amino]-4-oxo-6-phenylhexanoyl-L-tryptophan
-
 Escherichia coli
captopril
-
 Escherichia coli
lisinopril
-
 Escherichia coli
lisinopril-Trp
-
 Escherichia coli
additional information inhibition mechanism, molecular modeling, overview Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics, overview Escherichia coli
0.00029
-
 o-aminobenzoyl-FRK(2,4-dinitrophenyl)P-OH pH 8.0, 37°C, recombinant enzyme Escherichia coli
0.00066
-
 o-aminobenzoyl-FRAK(2,4-dinitrophenyl)-OH pH 8.0, 37°C, recombinant enzyme Escherichia coli
0.00255
-
 o-aminobenzoyl-LFK(2,4-dinitrophenyl)-OH pH 8.0, 37°C, recombinant enzyme Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Escherichia coli EcDcp removes dipeptides from the free C-termini of peptides, N-blocked tripeptides and unprotected tetrapeptides ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P24171
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged Dcp from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
angiotensin I + H2O i.e. DRVYIHPFHL, hydrolysis at the F-H bond generating angiotensin II Escherichia coli DRVYIHPF + His-Leu
-
 ?
bradykinin + H2O i.e. RPPGFSPFR, hydrolysis at at sequential cleavage sites releasing the C-terminal dipeptides F-R and S-P Escherichia coli RPPGF + Ser-Pro + Phe-Arg
-
 ?
additional information EcDcp removes dipeptides from the free C-termini of peptides, N-blocked tripeptides and unprotected tetrapeptides Escherichia coli ?
-
 ?
additional information no activity with Ac-SDKP-OH, o-aminobenzoyl-FRAK(2,4-dinitrophenyl)-NH2, o-aminobenzoyl-SDK(2,4-dinitrophenyl)P-OH and o-aminobenzoyl-APK(2,4-dinitrophenyl)-OH Escherichia coli ?
-
 ?
o-aminobenzoyl-FRAK(2,4-dinitrophenyl)-OH + H2O an ACE substrate, Dcp hydrolyzes at the R-A bond Escherichia coli o-aminobenzoyl-FR + AK(2,4-dinitrophenyl)-OH
-
 ?
o-aminobenzoyl-FRK(2,4-dinitrophenyl)P-OH + H2O an ACE substrate, Dcp hydrolyzes at the R-K bond Escherichia coli o-aminobenzoyl-FR + K(2,4-dinitrophenyl)P-OH
-
 ?
o-aminobenzoyl-LFK(2,4-dinitrophenyl)-OH + H2O an ACE substrate, Dcp hydrolyzes at the L-F bond Escherichia coli o-aminobenzoyl-L + FK(2,4-dinitrophenyl)-OH
-
 ?

Subunits

Subunits Comment Organism
More peptide mapping, overview Escherichia coli

Synonyms

Synonyms Comment Organism
DCP
-
 Escherichia coli
Dipeptidyl carboxypeptidase
-
 Escherichia coli
dipeptidyl carboxypeptidase II
-
 Escherichia coli
More the enzyme belongs to the metallopeptidase family M3 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.81
-
 o-aminobenzoyl-FRK(2,4-dinitrophenyl)P-OH pH 8.0, 37°C, recombinant enzyme Escherichia coli
1.1
-
 o-aminobenzoyl-FRAK(2,4-dinitrophenyl)-OH pH 8.0, 37°C, recombinant enzyme Escherichia coli
1.4
-
 o-aminobenzoyl-LFK(2,4-dinitrophenyl)-OH pH 8.0, 37°C, recombinant enzyme Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
4 10 activity range, profile, overview Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information inhibition kinetics, overview Escherichia coli
0.000003
-
 captopril
-
 Escherichia coli
0.0008
-
 lisinopril-Trp
-
 Escherichia coli
0.0044
-
 lisinopril
-
 Escherichia coli
0.022
-
 (5S)-5-[(N-benzoyl)amino]-4-oxo-6-phenylhexanoyl-L-tryptophan
-
 Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
538
-
 o-aminobenzoyl-LFK(2,4-dinitrophenyl)-OH pH 8.0, 37°C, recombinant enzyme Escherichia coli
1667
-
 o-aminobenzoyl-FRAK(2,4-dinitrophenyl)-OH pH 8.0, 37°C, recombinant enzyme Escherichia coli
2793
-
 o-aminobenzoyl-FRK(2,4-dinitrophenyl)P-OH pH 8.0, 37°C, recombinant enzyme Escherichia coli