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Literature summary for 3.4.15.1 extracted from

  • Hayakari, M.; Satoh, K.; Izumi, H.; Kudoh, T.; Asano, J.; Yamazaki, T.; Tsuchida, S.
    Kinetic-controlled hydrolysis of Leu-Val-Val-hemorphin-7 catalyzed by angiotensin-converting enzyme from rat brain (2003), Peptides, 24, 1075-1082.
    View publication on PubMed
Search for more literature for 3.4.15.1

Activating Compound

Activating Compound Comment Organism Structure
Cl- activation, optimal NaCl concentration for the brain enzyme in hydrolysis of LVVYPWTQRY is 10 mM, optimal concentration for hydrolysis of angiotensin I is 200 mM Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.014
-
 LVVYPWTQRY pH 7.5, 37°C, enzyme from testis, 200 mM NaCl Rattus norvegicus
0.015
-
 LVVYPWTQRY pH 7.5, 37°C, enzyme from brain, 200 mM NaCl Rattus norvegicus
0.019
-
 LVVYPWTQRY pH 7.5, 37°C, enzyme from brain, 10 mM NaCl Rattus norvegicus
0.033
-
 LVVYPWTQRY pH 7.5, 37°C, enzyme from testis, 10 mM NaCl Rattus norvegicus
0.04
-
 angiotensin I pH 7.5, 37°C, enzyme from brain, 200 mM NaCl Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Rattus norvegicus
-
testis
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
angiotensin I + H2O
-
 Rattus norvegicus angiotensin II + His-Leu
-
 ?
LVVYPWTQRY + H2O
-
 Rattus norvegicus LVVYPWTQ + RY + LVVY + PW + LVVYPW + TQ dipeptide TQ is unidentified. Sequential removal of dipeptides in three consecutive steps ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4
-
 angiotensin I pH 7.5, 37°C, enzyme from brain, 200 mM NaCl Rattus norvegicus
51
-
 LVVYPWTQRY pH 7.5, 37°C, enzyme from testis, 10 mM NaCl Rattus norvegicus
77
-
 LVVYPWTQRY pH 7.5, 37°C, enzyme from testis, 200 mM NaCl Rattus norvegicus
89
-
 LVVYPWTQRY pH 7.5, 37°C, enzyme from brain, 200 mM NaCl Rattus norvegicus
230
-
 LVVYPWTQRY pH 7.5, 37°C, enzyme from brain, 10 mM NaCl Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9.5 pH 5.5: 55% of maximal acticity, pH 50°C: about 50% of maximal activity, enzyme from brain, hydrolysis of LVVYPWTQRY Rattus norvegicus