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Literature summary for 3.4.14.9 extracted from

  • Vines, D.; Warburton, M.J.
    Purification and characterisation of a tripeptidyl aminopeptidase I from rat spleen (1998), Biochim. Biophys. Acta, 1384, 233-242.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Ala-Ala-Ala-CH2Cl competitive Rattus norvegicus
Ala-Ala-Phe-CH2Cl 0.001 mM, complete inhibition, competitive Rattus norvegicus
Ala-Ala-Tyr(benzoyl)-CH2Cl competitive Rattus norvegicus
Ala-Ala-Tyr-CH2Cl competitive Rattus norvegicus
Ala-Phe-Ala-CH2Cl competitive Rattus norvegicus
bestatin 0.01 mM, 11% inhibition Rattus norvegicus
diethyl dicarbonate 61% inhibition with 1 mM, 91% inhibition with 10 mM Rattus norvegicus
E-64 0.01 mM, 22% inhibition Rattus norvegicus
leupeptin 0.001 mg/ml, 17% inhibition Rattus norvegicus
pepstatin A 0.1 mg/ml, 26% inhibition Rattus norvegicus
Phe-Ala-Ala-CH2Cl competitive Rattus norvegicus
Phe-CH2Cl 0.1 mM, 21% inhibition Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.73
-
Ala-Ala-Phe-7-amido-4-methylcoumarin pH 4.0 Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
1 * 47000, SDS-PAGE Rattus norvegicus
64000
-
gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
spleen
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12.6
-
-
Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Ala-Ala-4-nitroanilide + H2O
-
Rattus norvegicus Ala-Ala-Ala + 4-nitroaniline
-
?
Ala-Ala-Phe-4-nitroanilide + H2O
-
Rattus norvegicus Ala-Ala-Phe + 4-nitroaniline
-
?
Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O
-
Rattus norvegicus Ala-Ala-Phe + 7-amino-4-methylcoumarin
-
?
DRVYIHPF + H2O
-
Rattus norvegicus DRV + YIHPF
-
?
glucagon + H2O
-
Rattus norvegicus additional information all nine tripeptides and the C-terminal pentapeptide are identified ?
Gly-Pro-Leu 2-naphthylamide + H2O 25% of the activity with Gly-Pro-Met 2-naphthylamide, Ala-Ala-Phe-4-nitroanilide or Ala-Ala-Phe-7-amido-4-methylcoumarin Rattus norvegicus Gly-Pro-Leu + 2-naphthylamine
-
?
Gly-Pro-Met 2-naphthylamide + H2O
-
Rattus norvegicus Gly-Pro-Met + 2-naphthylamine
-
?
additional information the enzymne cleaves tripeptides from synthetic substrates provided that the N-terminus is unsubstituted and the amino acid in the P1 position is not charged. The enzyme also cleaves small peptides, angiotensin II and glucagon, releasing tripeptides but does not appear to demonstrate any preference for amino acids on either side of the cleavage site. Substrates with a charged amino acid in the P1 position appear to be resistant to hydrolysis Rattus norvegicus ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 47000, SDS-PAGE Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
hydrolysis of Ala-Ala-Phe-7-amido-4-methylcoumarin Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
3.5 5 pH 3.5: about 25% of maximal activity, pH 5.0: about 45% of maximal activity, hydrolysis of Ala-Ala-Phe-7-amido-4-methylcoumarin Rattus norvegicus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 6 48 h, stable Rattus norvegicus
7
-
48 h, unstable above Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000063
-
Ala-Ala-Phe-CH2Cl pH 4.0, 37°C Rattus norvegicus
0.00072
-
Ala-Ala-Tyr-CH2Cl pH 4.0, 37°C Rattus norvegicus
0.00081
-
Ala-Ala-Tyr(benzoyl)-CH2Cl pH 4.0, 37°C Rattus norvegicus
0.0013
-
Phe-Ala-Ala-CH2Cl pH 4.0, 37°C Rattus norvegicus
0.0069
-
Ala-Ala-Ala-CH2Cl pH 4.0, 37°C Rattus norvegicus
0.0079
-
Ala-Phe-Ala-CH2Cl pH 4.0, 37°C Rattus norvegicus