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Literature summary for 3.4.14.9 extracted from

  • Du, P.G.; Kato, S.; Li, Y.H.; Maeda, T.; Yamane, T.; Yamamoto, S.; Fujiwara, M.; Yamamoto, Y.; Nishi, K.; Ohkubo, I.
    Rat tripeptidyl peptidase I: molecular cloning, functional expression, tissue localization and enzymatic characterization (2001), Biol. Chem., 382, 1715-1725.
    View publication on PubMed
Search for more literature for 3.4.14.9

Cloned(Commentary)

Cloned (Comment) Organism
-
 Rattus norvegicus

General Stability

General Stability Organism
resistant against freezing and thawing Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
Ala-Ala-Phe-CH2Cl IC50: 0.06 mM Rattus norvegicus
diisopropylfluorophosphate IC50: 1.15 mM Rattus norvegicus
Diprotin A 0.1 mM, 50% inhibition Rattus norvegicus
Hg2+ 1 mM, complete inhibition, IC50: 0.67 mM Rattus norvegicus
p-chloromercuribenzene sulfonic acid IC50: 0.27 mM Rattus norvegicus
pepstatin 0.1 mM, 15% inhibition Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.68
-
 Ala-Ala-Phe-7-amido-4-methylcoumarin pH 4.0 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
lysosome
-
 Rattus norvegicus 5764
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
 x * 46000, SDS-PAGE in presence of 2-mercaptoethanol Rattus norvegicus
280000
-
 non-denaturing PAGE Rattus norvegicus
290000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Rattus norvegicus an inherited deficiency of tripeptidyl peptidase I activity causes a fatal lysosomal storage disorder, classic late infantile neuronal ceroid lipofuscinosis, CLN2 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
adrenal gland
-
 Rattus norvegicus
-
bronchus
-
 Rattus norvegicus
-
cerebellum activity in neuroglia cells, no activity in small granule cells, Purkinje cells, remified dendrites and myelinated fibers Rattus norvegicus
-
cerebrum
-
 Rattus norvegicus
-
epididymis
-
 Rattus norvegicus
-
esophagus
-
 Rattus norvegicus
-
heart
-
 Rattus norvegicus
-
kidney activity in proximal tubules, distal tubules, collecting tubules and transitional cells, no activity in glomerulus and Bowman‘s capsule Rattus norvegicus
-
large intestine
-
 Rattus norvegicus
-
liver
-
 Rattus norvegicus
-
lung
-
 Rattus norvegicus
-
ovary activity in corpus luteum and corpus albicus, no activity in follicles Rattus norvegicus
-
pancreas activity in ducts, no activity in acinar cells and islets of Langerhans Rattus norvegicus
-
prostate
-
 Rattus norvegicus
-
salivary gland activity in excretory duct and stereocilia, no activity in glandular acinus and intercalated portion Rattus norvegicus
-
seminal vesicle
-
 Rattus norvegicus
-
small intestine
-
 Rattus norvegicus
-
spleen activity in red pulp, no activity in white pulp Rattus norvegicus
-
stomach
-
 Rattus norvegicus
-
testis activity in spermatogonia, spermatozoa, Sertoli cells and Leygig cells, no activity in basement cells Rattus norvegicus
-
thymus
-
 Rattus norvegicus
-
trachea
-
 Rattus norvegicus
-
uterus
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.583
-
-
 Rattus norvegicus

Storage Stability

Storage Stability Organism
4°C, pH 4.0, 50 mM sodium acetate buffer, stable for 10-14 days Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(pGlu)LYENKPRRRPYIL + H2O i.e. neurotensin, 15.0% degradation after 16 h Rattus norvegicus (pGlu)L + YENKPRRRPYIL
-
 ?
Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O 33.8% degradation within 60 min Rattus norvegicus Ala-Ala-Phe + 7-amino-4-methylcoumarin
-
 ?
DRVYIHPF + H2O i.e. angiotensin II, 18.2% degradation after 16 h Rattus norvegicus DRV + YIHPF
-
 ?
DRVYIHPFHL + H2O i.e. angiotensin I, 11.3% degradation after 16 h Rattus norvegicus DRV + YIHPFHL
-
 ?
GKPIPFFRLK + H2O endo-type substrate, 24.5% degradation after 16 h Rattus norvegicus GKPIP + FFRLK
-
 ?
GNLWATGHFM-NH2 + H2O i.e. neuromedin B, complete degradation after 16 h Rattus norvegicus GNL + WATGHFM-NH2
-
 ?
additional information substrates not cleaved after 24 h of digestion: dynorphin, beta-casomorphin-7, Leu-enkephalin, Met-enkephalin, neurokinin-A, LVV-hemorphin, bradykinin Rattus norvegicus ?
-
 ?
additional information an inherited deficiency of tripeptidyl peptidase I activity causes a fatal lysosomal storage disorder, classic late infantile neuronal ceroid lipofuscinosis, CLN2 Rattus norvegicus ?
-
 ?
RVYIHPF + H2O i.e. angiotensin III, 95.0% degradation after 15 min Rattus norvegicus RVY + IHPF
-
 ?
YGGFLRKYP + H2O i.e. beta-neo-endorphin, 28% degradation after 16 h Rattus norvegicus YGG + FLRKYP
-
 ?

Subunits

Subunits Comment Organism
? x * 46000, SDS-PAGE in presence of 2-mercaptoethanol Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 hydrolysis of Ala-Ala-Phe-7-amido-4-methylcoumarin Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
33.1
-
 Ala-Ala-Phe-7-amido-4-methylcoumarin pH 4.0 Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5 4.5
-
 Rattus norvegicus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5 4.5 stable Rattus norvegicus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.06
-
 IC50: 0.06 mM Rattus norvegicus Ala-Ala-Phe-CH2Cl
0.27
-
 IC50: 0.27 mM Rattus norvegicus p-chloromercuribenzene sulfonic acid
0.67
-
 1 mM, complete inhibition, IC50: 0.67 mM Rattus norvegicus Hg2+
1.15
-
 IC50: 1.15 mM Rattus norvegicus diisopropylfluorophosphate