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Literature summary for 3.4.14.9 extracted from

  • Krimper, R.P.; Jones, T.H.
    Purification and characterization of tripeptidyl peptidase I from Dictyostelium discoideum (1999), Biochem. Mol. Biol. Int., 47, 1079-1088.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 0.6 mM, complete inactivation Dictyostelium discoideum
angiotensin II 0.3 mM, 22% inhibition Dictyostelium discoideum
angiotensin III 0.3 mM, 18% inhibition Dictyostelium discoideum
Aprotinin 0.08 mM, 60% loss of activity Dictyostelium discoideum
dithiothreitol 0.6 mM, complete inactivation Dictyostelium discoideum
EDTA moderate Dictyostelium discoideum
glutathione 0.6 mM, 93% loss of activity Dictyostelium discoideum
Gly-Ala-Ala 0.3 mM, 36% inhibition Dictyostelium discoideum
Gly-His-Lys 0.3 mM, 49% inhibition Dictyostelium discoideum
Leu-Ala-Pro 0.3 mM, 55% inhibition Dictyostelium discoideum
Lys-Ala-Ala 0.3 mM, 34% inhibition Dictyostelium discoideum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0268
-
Ala-Ala-Phe-4-nitroanilide pH 4.5 Dictyostelium discoideum
0.437
-
Ala-Phe-Pro-p-nitroanilide pH 4.5 Dictyostelium discoideum
0.888
-
Ala-Ala-Pro-4-nitroanilide pH 4.5 Dictyostelium discoideum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
107000
-
2 * 107000, SDS-PAGE Dictyostelium discoideum
217000
-
gel filtration Dictyostelium discoideum

Organism

Organism UniProt Comment Textmining
Dictyostelium discoideum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Dictyostelium discoideum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
171
-
-
Dictyostelium discoideum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Ala-Ala-4-nitroanilide + H2O
-
Dictyostelium discoideum Ala-Ala-Ala + 4-nitroaniline
-
?
Ala-Ala-Phe-4-nitroanilide + H2O
-
Dictyostelium discoideum Ala-Ala-Phe + 4-nitroaniline
-
?
Ala-Ala-Pro-4-nitroanilide + H2O
-
Dictyostelium discoideum Ala-Ala-Pro + 4-nitroaniline
-
?
Ala-Phe-Pro-4-nitroanilide + H2O
-
Dictyostelium discoideum Ala-Phe-Pro + 4-nitroaniline
-
?
additional information no significant aminopeptidase activity Dictyostelium discoideum ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 107000, SDS-PAGE Dictyostelium discoideum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
140000
-
Ala-Phe-Pro-p-nitroanilide pH 4.5 Dictyostelium discoideum
210000
-
Ala-Ala-Pro-4-nitroanilide pH 4.5 Dictyostelium discoideum
550000
-
Ala-Ala-Phe-4-nitroanilide pH 4.5 Dictyostelium discoideum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
and a second optimum at pH 5.9 Dictyostelium discoideum
5.9
-
and a second optimum at pH 4.5, at pH 5.9 70% of the activity at pH 4.5 Dictyostelium discoideum