Activating Compound | Comment | Organism | Structure |
---|---|---|---|
bestatin | 0.2 mM, 10% activation | Streptococcus anginosus | |
EDTA | 1.0 mM, 8% activation | Streptococcus anginosus | |
leupeptin | 0.2 mM, 8% activation | Streptococcus anginosus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3,4-dichloroisocoumarine | 1.0 mM, 97% inhibition | Streptococcus anginosus | |
antipain | 0.2 mM, 17% inhibition | Streptococcus anginosus | |
Cu2+ | 1.0 mM, 15% inhibition | Streptococcus anginosus | |
diisopropylfluorophosphate | 1.0 mM, complete inhibition | Streptococcus anginosus | |
Mn2+ | 1.0 mM, 22% inhibition | Streptococcus anginosus | |
SDS | 1.0% complete inhibition | Streptococcus anginosus | |
Triton X-100 | 1.0%, 32% inhibition | Streptococcus anginosus | |
Zn2+ | 1.0 mM, 22% inhibition | Streptococcus anginosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.179 | - |
Met-Pro-4-nitroanilide | pH 7.5, 37°C | Streptococcus anginosus | |
0.217 | - |
His-Pro-4-nitroanilide | pH 7.5, 37°C | Streptococcus anginosus | |
0.217 | - |
Ser-Pro-4-nitroanilide | pH 7.5, 37°C | Streptococcus anginosus | |
0.33 | - |
Phe-Pro-4-nitroanilide | pH 7.5, 37°C | Streptococcus anginosus | |
0.37 | - |
Lys-Pro-4-nitroanilide | pH 7.5, 37°C | Streptococcus anginosus | |
0.556 | - |
Gly-Pro-4-nitroanilide | pH 7.5, 37°C | Streptococcus anginosus | |
0.714 | - |
Glu-Pro-4-nitroanilide | pH 7.5, 37°C | Streptococcus anginosus | |
0.75 | - |
Ala-Pro-4-nitroanilide | pH 7.5, 37°C | Streptococcus anginosus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 1.0 mM, 7% activation | Streptococcus anginosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
84000 | - |
x * 84000, SDS-PAGE | Streptococcus anginosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus anginosus | - |
NCTC 10713 | - |
Purification (Comment) | Organism |
---|---|
- |
Streptococcus anginosus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.75 | - |
- |
Streptococcus anginosus |
Storage Stability | Organism |
---|---|
-40°C, stable for at least 1 month | Streptococcus anginosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ala-Pro-4-nitroanilide + H2O | - |
Streptococcus anginosus | Ala-Pro + 4-nitroaniline | - |
? | |
Glu-Pro-4-nitroanilide + H2O | - |
Streptococcus anginosus | Glu-Pro + 4-nitroaniline | - |
? | |
Gly-Pro-4-nitroanilide + H2O | - |
Streptococcus anginosus | Gly-Pro + 4-nitroaniline | - |
? | |
His-Pro-4-nitroanilide + H2O | - |
Streptococcus anginosus | His-Pro + 4-nitroaniline | - |
? | |
Lys-Pro-4-nitroanilide + H2O | - |
Streptococcus anginosus | Lys-Pro + 4-nitroaniline | - |
? | |
Met-Pro-4-nitroanilide + H2O | - |
Streptococcus anginosus | Met-Pro + 4-nitroaniline | - |
? | |
additional information | no hydrolysis of Ala-Ala-Pro-4-nitroanilide, Ala-Phe-Pro-4-nitroanilide, Pro-4-nitroanilide, Leu-4-nitroanilide, Arg, 4-nitroanilide, Ala-Ala-Ala-4-nitroanilide | Streptococcus anginosus | ? | - |
? | |
Phe-Pro-4-nitroanilide + H2O | - |
Streptococcus anginosus | Phe-Pro + 4-nitroaniline | - |
? | |
Ser-Pro-4-nitroanilide + H2O | - |
Streptococcus anginosus | Ser-Pro + 4-nitroaniline | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 84000, SDS-PAGE | Streptococcus anginosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
10 h, stable | Streptococcus anginosus |
50 | - |
15 min, 94% loss of activity | Streptococcus anginosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Streptococcus anginosus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Streptococcus anginosus | isoelectric focusing, pH-range 4.0-6.0 | - |
4.9 |