Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-(2-aminoethyl)benzenesulfonyl fluoride | i.e. AEBSF | Hordeum vulgare | |
Diprotin A | specific inhibition | Hordeum vulgare | |
Diprotin B | specific inhibition | Hordeum vulgare | |
additional information | no inhibition by EDTA, E-64, pepstatin A or leupeptin | Hordeum vulgare | |
PMSF | - |
Hordeum vulgare |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000071 | - |
Lys-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
0.00009 | - |
Arg-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
0.00034 | - |
Ala-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
0.00065 | - |
Phe-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
0.0007 | - |
Ile-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
0.00073 | - |
Tyr-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
0.00093 | - |
L-Thr-L-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
0.00103 | - |
His-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
0.00109 | - |
Leu-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
0.0011 | - |
Pro-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
0.00126 | - |
Ser-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
0.00138 | - |
Gln-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
0.00217 | - |
Met-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
0.00298 | - |
Glu-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
0.00324 | - |
Asn-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
0.00374 | - |
Lys-Ala-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
0.00688 | - |
Gly-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
0.00888 | - |
Asp-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
85000 | - |
1 * 105000, SDS-PAGE, 1 * 85000, deglycosylated enzyme, SDS-PAGE | Hordeum vulgare |
105000 | - |
gel filtration | Hordeum vulgare |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hordeum vulgare | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the deglycosylated enzyme is still active | Hordeum vulgare |
Purification (Comment) | Organism |
---|---|
from green barley malt, more than 43000fold | Hordeum vulgare |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
release of an N-terminal dipeptide, Xaa-Yaa-/-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline | serine protease | Hordeum vulgare |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | malt from green barley | Hordeum vulgare | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
purified enzyme | Hordeum vulgare |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ala-Pro-7-amido-4-methylcoumarin + H2O | - |
Hordeum vulgare | Ala-Pro + 7-amino-4-methylcoumarin | - |
? | |
Arg-Pro-7-amido-4-methylcoumarin + H2O | high activity | Hordeum vulgare | Arg-Pro + 7-amino-4-methylcoumarin | - |
? | |
Asn-Pro-7-amido-4-methylcoumarin + H2O | - |
Hordeum vulgare | Asn-Pro + 7-amino-4-methylcoumarin | - |
? | |
Asp-Pro-7-amido-4-methylcoumarin + H2O | low activity | Hordeum vulgare | Asp-Pro + 7-amino-4-methylcoumarin | - |
? | |
beta-casomorphin + H2O | cleavage sites: YP-/-FP-/-GP-/-IGP-/-RP | Hordeum vulgare | fragments | - |
? | |
des-Pro2-bradykinin + H2O | cleavage sites: RP-/-GFSPFR | Hordeum vulgare | fragments | - |
? | |
Gln-Pro-7-amido-4-methylcoumarin + H2O | - |
Hordeum vulgare | Gln-Pro + 7-amino-4-methylcoumarin | - |
? | |
Glu-Pro-7-amido-4-methylcoumarin + H2O | low activity | Hordeum vulgare | Glu-Pro + 7-amino-4-methylcoumarin | - |
? | |
Gly-Pro-7-amido-4-methylcoumarin + H2O | - |
Hordeum vulgare | Gly-Pro + 7-amino-4-methylcoumarin | - |
? | |
His-Pro-7-amido-4-methylcoumarin + H2O | - |
Hordeum vulgare | His-Pro + 7-amino-4-methylcoumarin | - |
? | |
Ile-Pro-7-amido-4-methylcoumarin + H2O | - |
Hordeum vulgare | Ile-Pro + 7-amino-4-methylcoumarin | - |
? | |
L-Thr-L-Pro-7-amido-4-methylcoumarin + H2O | - |
Hordeum vulgare | L-Thr-L-Pro + 7-amino-4-methylcoumarin | - |
? | |
Leu-Pro-7-amido-4-methylcoumarin + H2O | - |
Hordeum vulgare | Leu-Pro + 7-amino-4-methylcoumarin | - |
? | |
Lys-Ala-7-amido-4-methylcoumarin + H2O | - |
Hordeum vulgare | Lys-Ala + 7-amino-4-methylcoumarin | - |
? | |
Lys-Pro-7-amido-4-methylcoumarin + H2O | best substrate | Hordeum vulgare | Lys-Pro + 7-amino-4-methylcoumarin | - |
? | |
Met-Pro-7-amido-4-methylcoumarin + H2O | - |
Hordeum vulgare | Met-Pro + 7-amino-4-methylcoumarin | - |
? | |
additional information | substrate specificity study, no activity with bradykinin, Pro-7-amido-4-methylcoumarin, benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin, and benzyloxycarbonyl-Phe-Ala-7-amido-4-methylcoumarin | Hordeum vulgare | ? | - |
? | |
Phe-Pro-7-amido-4-methylcoumarin + H2O | low activity | Hordeum vulgare | Phe-Pro + 7-amino-4-methylcoumarin | - |
? | |
Pro-Pro-7-amido-4-methylcoumarin + H2O | - |
Hordeum vulgare | Pro-Pro + 7-amino-4-methylcoumarin | - |
? | |
Ser-Pro-7-amido-4-methylcoumarin + H2O | - |
Hordeum vulgare | Ser-Pro + 7-amino-4-methylcoumarin | - |
? | |
substance P + H2O | cleavage sites: RP-/-KP-/-QQFFGLM | Hordeum vulgare | substance P peptide fragments | - |
? | |
Tyr-Pro-7-amido-4-methylcoumarin + H2O | - |
Hordeum vulgare | Tyr-Pro + 7-amino-4-methylcoumarin | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 105000, SDS-PAGE, 1 * 85000, deglycosylated enzyme, SDS-PAGE | Hordeum vulgare |
Synonyms | Comment | Organism |
---|---|---|
dipeptidyl peptidase IV | - |
Hordeum vulgare |
DPPIV | - |
Hordeum vulgare |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Hordeum vulgare |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.8 | - |
Phe-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
4.6 | - |
Asp-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
10 | - |
Glu-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
10 | - |
Gln-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
22 | - |
Leu-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
23 | - |
Ala-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
35 | - |
Asn-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
41 | - |
His-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
41 | - |
Pro-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
58 | - |
Ser-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
60 | - |
Gly-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
67 | - |
Ile-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
71 | - |
Arg-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
90 | - |
Lys-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
92 | - |
Lys-Ala-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
99 | - |
Tyr-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
102 | - |
Met-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare | |
117 | - |
L-Thr-L-Pro-7-amido-4-methylcoumarin | pH 7.2, 25°C | Hordeum vulgare |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Hordeum vulgare |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | - |
Hordeum vulgare |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Hordeum vulgare | isoelectric focusing | - |
3.6 |