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Literature summary for 3.4.14.5 extracted from

  • Davy, A.; Thomsen, K.K.; Juliano, M.A.; Alves, L.C.; Svendsen, I.; Simpson, D.J.
    Purification and characterization of barley dipeptidyl peptidase IV (2000), Plant Physiol., 122, 425-432.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
4-(2-aminoethyl)benzenesulfonyl fluoride i.e. AEBSF Hordeum vulgare
Diprotin A specific inhibition Hordeum vulgare
Diprotin B specific inhibition Hordeum vulgare
additional information no inhibition by EDTA, E-64, pepstatin A or leupeptin Hordeum vulgare
PMSF
-
Hordeum vulgare

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000071
-
Lys-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
0.00009
-
Arg-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
0.00034
-
Ala-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
0.00065
-
Phe-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
0.0007
-
Ile-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
0.00073
-
Tyr-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
0.00093
-
L-Thr-L-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
0.00103
-
His-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
0.00109
-
Leu-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
0.0011
-
Pro-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
0.00126
-
Ser-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
0.00138
-
Gln-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
0.00217
-
Met-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
0.00298
-
Glu-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
0.00324
-
Asn-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
0.00374
-
Lys-Ala-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
0.00688
-
Gly-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
0.00888
-
Asp-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
85000
-
1 * 105000, SDS-PAGE, 1 * 85000, deglycosylated enzyme, SDS-PAGE Hordeum vulgare
105000
-
gel filtration Hordeum vulgare

Organism

Organism UniProt Comment Textmining
Hordeum vulgare
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the deglycosylated enzyme is still active Hordeum vulgare

Purification (Commentary)

Purification (Comment) Organism
from green barley malt, more than 43000fold Hordeum vulgare

Reaction

Reaction Comment Organism Reaction ID
release of an N-terminal dipeptide, Xaa-Yaa-/-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline serine protease Hordeum vulgare

Source Tissue

Source Tissue Comment Organism Textmining
seed malt from green barley Hordeum vulgare
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
purified enzyme Hordeum vulgare

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Pro-7-amido-4-methylcoumarin + H2O
-
Hordeum vulgare Ala-Pro + 7-amino-4-methylcoumarin
-
?
Arg-Pro-7-amido-4-methylcoumarin + H2O high activity Hordeum vulgare Arg-Pro + 7-amino-4-methylcoumarin
-
?
Asn-Pro-7-amido-4-methylcoumarin + H2O
-
Hordeum vulgare Asn-Pro + 7-amino-4-methylcoumarin
-
?
Asp-Pro-7-amido-4-methylcoumarin + H2O low activity Hordeum vulgare Asp-Pro + 7-amino-4-methylcoumarin
-
?
beta-casomorphin + H2O cleavage sites: YP-/-FP-/-GP-/-IGP-/-RP Hordeum vulgare fragments
-
?
des-Pro2-bradykinin + H2O cleavage sites: RP-/-GFSPFR Hordeum vulgare fragments
-
?
Gln-Pro-7-amido-4-methylcoumarin + H2O
-
Hordeum vulgare Gln-Pro + 7-amino-4-methylcoumarin
-
?
Glu-Pro-7-amido-4-methylcoumarin + H2O low activity Hordeum vulgare Glu-Pro + 7-amino-4-methylcoumarin
-
?
Gly-Pro-7-amido-4-methylcoumarin + H2O
-
Hordeum vulgare Gly-Pro + 7-amino-4-methylcoumarin
-
?
His-Pro-7-amido-4-methylcoumarin + H2O
-
Hordeum vulgare His-Pro + 7-amino-4-methylcoumarin
-
?
Ile-Pro-7-amido-4-methylcoumarin + H2O
-
Hordeum vulgare Ile-Pro + 7-amino-4-methylcoumarin
-
?
L-Thr-L-Pro-7-amido-4-methylcoumarin + H2O
-
Hordeum vulgare L-Thr-L-Pro + 7-amino-4-methylcoumarin
-
?
Leu-Pro-7-amido-4-methylcoumarin + H2O
-
Hordeum vulgare Leu-Pro + 7-amino-4-methylcoumarin
-
?
Lys-Ala-7-amido-4-methylcoumarin + H2O
-
Hordeum vulgare Lys-Ala + 7-amino-4-methylcoumarin
-
?
Lys-Pro-7-amido-4-methylcoumarin + H2O best substrate Hordeum vulgare Lys-Pro + 7-amino-4-methylcoumarin
-
?
Met-Pro-7-amido-4-methylcoumarin + H2O
-
Hordeum vulgare Met-Pro + 7-amino-4-methylcoumarin
-
?
additional information substrate specificity study, no activity with bradykinin, Pro-7-amido-4-methylcoumarin, benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin, and benzyloxycarbonyl-Phe-Ala-7-amido-4-methylcoumarin Hordeum vulgare ?
-
?
Phe-Pro-7-amido-4-methylcoumarin + H2O low activity Hordeum vulgare Phe-Pro + 7-amino-4-methylcoumarin
-
?
Pro-Pro-7-amido-4-methylcoumarin + H2O
-
Hordeum vulgare Pro-Pro + 7-amino-4-methylcoumarin
-
?
Ser-Pro-7-amido-4-methylcoumarin + H2O
-
Hordeum vulgare Ser-Pro + 7-amino-4-methylcoumarin
-
?
substance P + H2O cleavage sites: RP-/-KP-/-QQFFGLM Hordeum vulgare substance P peptide fragments
-
?
Tyr-Pro-7-amido-4-methylcoumarin + H2O
-
Hordeum vulgare Tyr-Pro + 7-amino-4-methylcoumarin
-
?

Subunits

Subunits Comment Organism
monomer 1 * 105000, SDS-PAGE, 1 * 85000, deglycosylated enzyme, SDS-PAGE Hordeum vulgare

Synonyms

Synonyms Comment Organism
dipeptidyl peptidase IV
-
Hordeum vulgare
DPPIV
-
Hordeum vulgare

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Hordeum vulgare

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8
-
Phe-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
4.6
-
Asp-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
10
-
Glu-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
10
-
Gln-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
22
-
Leu-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
23
-
Ala-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
35
-
Asn-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
41
-
His-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
41
-
Pro-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
58
-
Ser-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
60
-
Gly-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
67
-
Ile-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
71
-
Arg-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
90
-
Lys-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
92
-
Lys-Ala-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
99
-
Tyr-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
102
-
Met-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare
117
-
L-Thr-L-Pro-7-amido-4-methylcoumarin pH 7.2, 25°C Hordeum vulgare

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
Hordeum vulgare

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9
-
Hordeum vulgare

pI Value

Organism Comment pI Value Maximum pI Value
Hordeum vulgare isoelectric focusing
-
3.6