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Literature summary for 3.4.14.5 extracted from
- Hemorphins: substrates and/or inhibitors of dipeptidyl peptidase IV. Hemorphins N-terminus sequence influence on the interaction between hemorphins and DPPIV (2004), Biochimie, 86, 31-37.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Diprotin A | - |
Rattus norvegicus |  |
| hemorphin-4 peptide | 12% inhibition at 0.03 mM, endogenous blood-derived peptide belonging to the family of atypical opioidic peptides released from sequentially hydrolyzed hemoglobin, amino acid sequence YPWT, competitive | Rattus norvegicus | |
| hemorphin-5 peptide | 18% inhibition at 0.03 mM, endogenous blood-derived peptide belonging to the family of atypical opioidic peptides released from sequentially hydrolyzed hemoglobin, amino acid sequence YPWTQ, competitive | Rattus norvegicus | |
| hemorphin-7 peptide | 30% inhibition at 0.03 mM, endogenous blood-derived peptide belonging to the family of atypical opioidic peptides released from sequentially hydrolyzed hemoglobin, amino acid sequence YPWTQRF, selective and competitive | Rattus norvegicus | |
| LVV-hemorphin-7 peptide | 9% inhibition at 0.03 mM, endogenous blood-derived peptide belonging to the family of atypical opioidic peptides released from sequentially hydrolyzed hemoglobin, amino acid sequence LVVYPWTQRF, the additional leucine residue reduces the inhibitory potential | Rattus norvegicus | |
| additional information | inhibitory mechanism, the N-terminus sequence of hemorphins influence the interaction between hemorphins and the enzyme | Rattus norvegicus | |
| VV-hemorphin-5 peptide | 10% inhibition at 0.03 mM, endogenous blood-derived peptide belonging to the family of atypical opioidic peptides released from sequentially hydrolyzed hemoglobin, amino acid sequence VVYPWTQ | Rattus norvegicus | |
| VV-hemorphin-6 peptide | 15% inhibition at 0.03 mM, endogenous blood-derived peptide belonging to the family of atypical opioidic peptides released from sequentially hydrolyzed hemoglobin, amino acid sequence VVYPWTQR | Rattus norvegicus | |
| VV-hemorphin-7 peptide | 27% inhibition at 0.03 mM, endogenous blood-derived peptide belonging to the family of atypical opioidic peptides released from sequentially hydrolyzed hemoglobin, amino acid sequence VVYPWTQRF | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0387 | - |
hemorphin-7 | pH 8.0, 37°C, microsomes | Rattus norvegicus | |
| 0.38 | - |
Gly-Pro-4-nitroanilide | pH 8.0, 37°C, microsomes | Rattus norvegicus | |
| 6.4 | - |
hemorphin-5 | pH 8.0, 37°C, microsomes | Rattus norvegicus | |
| 13.5 | - |
hemorphin-4 | pH 8.0, 37°C, microsomes | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Rattus norvegicus | 16020 | - |
| microsome | - |
Rattus norvegicus | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Rattus norvegicus | hemorphins may represent endogenous regulators of the enzyme activity | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
male Wistar rats | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, preparation of microsomal fraction | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Gly-Pro-4-nitroanilide + H2O | - |
Rattus norvegicus | Gly-Pro + 4-nitroaniline | - |
? | |
| hemorphin-4 + H2O | - |
Rattus norvegicus | YP + WT | - |
? | |
| hemorphin-5 + H2O | - |
Rattus norvegicus | YP + WTQ | - |
? | |
| hemorphin-7 + H2O | - |
Rattus norvegicus | YP + WTQRF | - |
? | |
| additional information | LVV-hemorphin-7 and LVV-hemorphin-6 are no substrates for the enzyme | Rattus norvegicus | ? | - |
? | |
| additional information | hemorphins may represent endogenous regulators of the enzyme activity | Rattus norvegicus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| dipeptidyl peptidase IV | - |
Rattus norvegicus |
| DPPIV | - |
Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.3 | - |
hemorphin-7 | pH 8.0, 37°C, microsomes | Rattus norvegicus | |
| 52.3 | - |
Gly-Pro-4-nitroanilide | pH 8.0, 37°C, microsomes | Rattus norvegicus | |
| 1770 | - |
hemorphin-4 | pH 8.0, 37°C, microsomes | Rattus norvegicus | |
| 1774 | - |
hemorphin-4 | pH 8.0, 37°C, microsomes | Rattus norvegicus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Rattus norvegicus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | inhibition kinetics | Rattus norvegicus | |
| 0.029 | - |
VV-hemorphin-7 peptide | pH 8.0, 37°C, microsomes | Rattus norvegicus | |
| 0.033 | - |
hemorphin-7 peptide | pH 8.0, 37°C, microsomes | Rattus norvegicus | |
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