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Literature summary for 3.4.14.5 extracted from

  • Baer, J.W.; Gerhartz, B.; Hoffmann, T.; Rosche, F.; Demuth, H.U.
    Characterisation of human DP IV produced by a Pichia pastoris expression system (2003), Adv. Exp. Med. Biol., 524, 103-108.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of the soluble His-tagged protein in Pichia pastoris strain X-33 under control of AOX1 promotor, removal of the membrane anchor, residues 1-36, secretion of the recombinant enzyme extracellular part into the cell culture medium and the periplasm Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
isoleucyl-(2)-cyanopyrrolidine
-
Homo sapiens
isoleucyl-thiazolidine
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.063
-
Gly-Pro-7-amido-4-methylcoumarin pH 7.6, 30°C, recombinant soluble enzyme Homo sapiens
0.165
-
Gly-Pro-4-nitroanilide pH 7.6, 30°C, recombinant soluble enzyme Homo sapiens
2.74
-
Ala-Ala-4-nitroanilide pH 7.6, 30°C, recombinant soluble enzyme Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane type II transmembrane protein Homo sapiens 5886
-
soluble
-
Homo sapiens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
103600
-
2 * 103600, recombinant soluble enzyme, MALDI-TOF mass spectrometry Homo sapiens
210000
-
about, recombinant soluble enzyme, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens enzyme is involved in diabetes mellitus, AIDS, and immunoregulatory processes ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein heterologous glycosylation, several isozymes Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant soluble His-tagged extracellular part of the enzyme from Pichia pastoris culture medium, 534fold Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
release of an N-terminal dipeptide, Xaa-Yaa-/-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline serine protease Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
32.3
-
purified recombinant soluble enzyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Ala-4-nitroanilide + H2O
-
Homo sapiens Ala-Ala + 4-nitroaniline
-
?
Gly-Pro-4-nitroanilide + H2O
-
Homo sapiens Gly-Pro + 4-nitroaniline
-
?
Gly-Pro-7-amido-4-methylcoumarin + H2O
-
Homo sapiens Gly-Pro + 7-amino-4-methylcoumarin
-
?
additional information enzyme is involved in diabetes mellitus, AIDS, and immunoregulatory processes Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 103600, recombinant soluble enzyme, MALDI-TOF mass spectrometry Homo sapiens

Synonyms

Synonyms Comment Organism
dipeptidyl peptidase IV
-
Homo sapiens
DP IV
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
assay at Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000038
-
isoleucyl-(2)-cyanopyrrolidine pH 7.6, 30°C, recombinant soluble enzyme Homo sapiens
0.000085
-
isoleucyl-thiazolidine pH 7.6, 30°C, recombinant soluble enzyme Homo sapiens

pI Value

Organism Comment pI Value Maximum pI Value
Homo sapiens several isozymes due to heterologous glycosylation, isoelectric focusing
-
5.47