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Literature summary for 3.4.14.4 extracted from

  • Hromic-Jahjefendic, A.; Jajcanin Jozic, N.; Kazazic, S.; Grabar Branilovic, M.; Karacic, Z.; Schrittwieser, J.H.; Das, K.M.P.; Tomin, M.; Oberer, M.; Gruber, K.; Abramic, M.; Tomic, S.
    A novel Porphyromonas gingivalis enzyme An atypical dipeptidyl peptidase III with an ARM repeat domain (2017), PLoS ONE, 12, e0188915 .
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00097
-
Arg-Arg-2-naphthylamide pH 8.0, 37°C Porphyromonas gingivalis
0.00256
-
Arg-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C Porphyromonas gingivalis

Organism

Organism UniProt Comment Textmining
Porphyromonas gingivalis Q7MX92
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Arg-2-naphthylamide + H2O
-
Porphyromonas gingivalis Ala-Arg + 2-naphthylamine
-
?
Arg-Arg-2-naphthylamide + H2O
-
Porphyromonas gingivalis Arg-Arg + 2-naphthylamine
-
?
Arg-Arg-4-methylcoumarin 7-amide + H2O
-
Porphyromonas gingivalis Arg-Arg + 7-amino-4-methylcoumarin
-
?
DRVYIHPF + H2O i.e. angiotensin II Porphyromonas gingivalis IHPF + ?
-
?
IVYPW + H2O
-
Porphyromonas gingivalis YPW + ?
-
?
Phe-Arg-2-naphthylamide + H2O
-
Porphyromonas gingivalis Phe-Arg + 2-naphthylamine
-
?
VVYPW + H2O i.e. tynorphin Porphyromonas gingivalis YPW + ?
-
?

Subunits

Subunits Comment Organism
? x * 102000, SDS-PAGE Porphyromonas gingivalis

Synonyms

Synonyms Comment Organism
PG_0317
-
Porphyromonas gingivalis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.29
-
Arg-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C Porphyromonas gingivalis
0.75
-
Arg-Arg-2-naphthylamide pH 8.0, 37°C Porphyromonas gingivalis

General Information

General Information Comment Organism
physiological function the enzyme possesses a C-terminal extension containing an Armadillo type fold similar to the AlkD family of bacterial DNA glycosylases. The Km values for both Arg-Arg substrates are increased by 4fold when the C-terminal domain is removed. kcat values are reduced 120fold in the case of Arg-Arg-4-methylcoumarin 7-amide, and 250fold in the case of Arg-Arg-2-naphthylamide, resulting in a 470fold and 1050fold reduction in the catalytic efficiency, respectively. Complementation assays in a DNA repair-deficient Escherichia coli strain indicate the absence of alkylation repair function Porphyromonas gingivalis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
113
-
Arg-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C Porphyromonas gingivalis
773
-
Arg-Arg-2-naphthylamide pH 8.0, 37°C Porphyromonas gingivalis