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Literature summary for 3.4.14.4 extracted from

  • Jajcanin-Jozic, N.; Tomic, S.; Abramic, M.
    Importance of the three basic residues in the vicinity of the zinc-binding motifs for the activity of the yeast dipeptidyl peptidase III (2014), J. Biochem., 155, 43-50.
    View publication on PubMed
Search for more literature for 3.4.14.4

Protein Variants

Protein Variants Comment Organism
H578D mutation 122fold lowers the catalytic efficiency for Arg-Arg 2-naphthylamide hydrolysis, and 14fold decreases affinity for hydroxamate inhibitor Tyr-Phe-NHOH Saccharomyces cerevisiae
K638L mutation slightly increases the specificity constant for Arg-Arg 2-naphthylamide hydrolysis. The affinity for Tyr-Phe-NHOH, and activity for the substrates with uncharged P2 side chains such as Ala-Ala-, Ala-Arg- and Phe-Arg 2-naphthylamide are dramatically reduced Saccharomyces cerevisiae
R582Q mutant exhibits an order of magnitude higher activity with all four dipeptide derivatives examined, compared to the wild type, due to a change in the H-bond networking in the R582Q variant active-site region Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0026
-
 L-Arg-L-Arg 2-naphthylamide mutant R582Q, pH 8.0, 25°C, presence of CoCl2 Saccharomyces cerevisiae
0.0032
-
 L-Arg-L-Arg 2-naphthylamide mutant R582Q, pH 8.0, 25°C, absence of CoCl2 Saccharomyces cerevisiae
0.004
-
 L-Arg-L-Arg 2-naphthylamide mutant K638L, pH 8.0, 25°C, absence of CoCl2 Saccharomyces cerevisiae
0.0051
-
 L-Arg-L-Arg 2-naphthylamide mutant K638L, pH 8.0, 25°C, presence of CoCl2 Saccharomyces cerevisiae
0.0143
-
 L-Arg-L-Arg 2-naphthylamide wild-type, pH 8.0, 25°C, absence of CoCl2 Saccharomyces cerevisiae
0.0152
-
 L-Arg-L-Arg 2-naphthylamide wild-type, pH 8.0, 25°C, presence of CoCl2 Saccharomyces cerevisiae
0.0251
-
 L-Arg-L-Arg 2-naphthylamide mutant H578N, pH 8.0, 25°C, presence of CoCl2 Saccharomyces cerevisiae
0.0945
-
 L-Ala-L-Ala 2-naphthylamide mutant R582Q, pH 8.0, 25°C, presence of CoCl2 Saccharomyces cerevisiae
0.1643
-
 L-Ala-L-Ala 2-naphthylamide mutant K638L, pH 8.0, 25°C, presence of CoCl2 Saccharomyces cerevisiae
0.2316
-
 L-Ala-L-Ala 2-naphthylamide wild-type, pH 8.0, 25°C, presence of CoCl2 Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae Q08225
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Ala-L-Ala 2-naphthylamide + H2O
-
 Saccharomyces cerevisiae L-Ala-L-Ala + 2-naphthylamine
-
 ?
L-Arg-L-Arg 2-naphthylamide + H2O
-
 Saccharomyces cerevisiae L-Arg-L-Arg + 2-naphthylamine
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.002
-
 L-Ala-L-Ala 2-naphthylamide mutant K638L, pH 8.0, 25°C, presence of CoCl2 Saccharomyces cerevisiae
0.003
-
 L-Arg-L-Arg 2-naphthylamide mutant H578N, pH 8.0, 25°C, presence of CoCl2 Saccharomyces cerevisiae
0.022
-
 L-Arg-L-Arg 2-naphthylamide mutant K638L, pH 8.0, 25°C, absence of CoCl2 Saccharomyces cerevisiae
0.028
-
 L-Arg-L-Arg 2-naphthylamide wild-type, pH 8.0, 25°C, absence of CoCl2 Saccharomyces cerevisiae
0.163
-
 L-Arg-L-Arg 2-naphthylamide mutant K638L, pH 8.0, 25°C, presence of CoCl2 Saccharomyces cerevisiae
0.248
-
 L-Arg-L-Arg 2-naphthylamide wild-type, pH 8.0, 25°C, presence of CoCl2 Saccharomyces cerevisiae
0.74
-
 L-Arg-L-Arg 2-naphthylamide mutant R582Q, pH 8.0, 25°C, absence of CoCl2 Saccharomyces cerevisiae
0.777
-
 L-Ala-L-Ala 2-naphthylamide wild-type, pH 8.0, 25°C, presence of CoCl2 Saccharomyces cerevisiae
0.953
-
 L-Arg-L-Arg 2-naphthylamide mutant R582Q, pH 8.0, 25°C, presence of CoCl2 Saccharomyces cerevisiae
7.25
-
 L-Ala-L-Ala 2-naphthylamide mutant R582Q, pH 8.0, 25°C, presence of CoCl2 Saccharomyces cerevisiae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.012
-
 L-Ala-L-Ala 2-naphthylamide mutant K638L, pH 8.0, 25°C, presence of CoCl2 Saccharomyces cerevisiae
0.133
-
 L-Arg-L-Arg 2-naphthylamide mutant H578N, pH 8.0, 25°C, presence of CoCl2 Saccharomyces cerevisiae
1.982
-
 L-Arg-L-Arg 2-naphthylamide wild-type, pH 8.0, 25°C, absence of CoCl2 Saccharomyces cerevisiae
3.26
-
 L-Ala-L-Ala 2-naphthylamide wild-type, pH 8.0, 25°C, presence of CoCl2 Saccharomyces cerevisiae
5.42
-
 L-Arg-L-Arg 2-naphthylamide mutant K638L, pH 8.0, 25°C, absence of CoCl2 Saccharomyces cerevisiae
16.34
-
 L-Arg-L-Arg 2-naphthylamide wild-type, pH 8.0, 25°C, presence of CoCl2 Saccharomyces cerevisiae
76.7
-
 L-Ala-L-Ala 2-naphthylamide mutant R582Q, pH 8.0, 25°C, presence of CoCl2 Saccharomyces cerevisiae
231
-
 L-Arg-L-Arg 2-naphthylamide mutant R582Q, pH 8.0, 25°C, absence of CoCl2 Saccharomyces cerevisiae
366.7
-
 L-Arg-L-Arg 2-naphthylamide mutant R582Q, pH 8.0, 25°C, presence of CoCl2 Saccharomyces cerevisiae
3202
-
 L-Arg-L-Arg 2-naphthylamide mutant K638L, pH 8.0, 25°C, presence of CoCl2 Saccharomyces cerevisiae