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Literature summary for 3.4.14.4 extracted from

  • Spoljaric, J.; Salopek-Sondi, B.; Makarevic, J.; Vukelic, B.; Agic, D.; Simaga, S.; Jajcanin-Jozic, N.; Abramic, M.
    Absolutely conserved tryptophan in M49 family of peptidases contributes to catalysis and binding of competitive inhibitors (2009), Bioorg. Chem., 37, 70-76.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 Homo sapiens

Protein Variants

Protein Variants Comment Organism
W300F mutant with slight increase in activity compared to wild-type enzyme Homo sapiens
W300L mutant with higher activity compared to wild-type enzyme Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
L-tyrosyl-glycine hydroxamic acid
-
Homo sapiens
L-tyrosyl-L-phenylalanine hydroxamic acid
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0116
-
Arg-Arg-2-naphthylamide wild-type, pH 8.0, 37°C Homo sapiens
0.0138
-
Arg-Arg-2-naphthylamide mutant W300F, pH 8.0, 37°C Homo sapiens
0.057
-
Arg-Arg-2-naphthylamide mutant W300L, pH 8.0, 37°C Homo sapiens
0.299
-
Ala-Ala-2-naphthylamide wild-type, pH 8.0, 37°C Homo sapiens
0.355
-
Ala-Ala-2-naphthylamide mutant W300F, pH 8.0, 37°C Homo sapiens
0.441
-
Ala-Ala-2-naphthylamide mutant W300L, pH 8.0, 37°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
82000
-
Western blot Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9NY33
-
-

Purification (Commentary)

Purification (Comment) Organism
about 28% yield after gel filtration and FPLC for wild-type enzyme Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.43
-
mutant W300L, pH 8.0, 37°C Homo sapiens
24.9
-
mutant W300F, pH 8.0, 37°C Homo sapiens
45.6
-
wild-type, pH 8.0, 37°C Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Ala-2-naphthylamide + H2O assay at 37°C, pH 8.0 Homo sapiens Ala-Ala + 2-naphtylamine
-
?
Ala-Phe-2-naphthylamide + H2O assay at 37°C, pH 8.0 Homo sapiens Ala-Phe + 2-naphtylamine
-
?
Arg-Arg-2-naphthylamide + H2O assay at 37°C, pH 8.0 Homo sapiens Arg-Arg + 2-naphthylamine
-
?
Gly-Phe-2-naphthylamide + H2O assay at 37°C, pH 8.0 Homo sapiens Gly-Phe + 2-naphthylamine
-
?

Synonyms

Synonyms Comment Organism
dipeptidyl peptidase III
-
Homo sapiens
DPP III
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
50% enzyme inactivation at 50°C for wild-type enzyme and mutant W300F and mutant W300L Homo sapiens
55
-
wild-type enzyme and mutant W300F and mutant W300L completely inactivated Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.02
-
Ala-Ala-2-naphthylamide mutant W300L, pH 8.0, 37°C Homo sapiens
0.085
-
Ala-Ala-2-naphthylamide mutant W300F, pH 8.0, 37°C Homo sapiens
0.35
-
Ala-Ala-2-naphthylamide wild-type, pH 8.0, 37°C Homo sapiens
1.4
-
Arg-Arg-2-naphthylamide mutant W300L, pH 8.0, 37°C Homo sapiens
10.7
-
Arg-Arg-2-naphthylamide mutant W300F, pH 8.0, 37°C Homo sapiens
21.8
-
Arg-Arg-2-naphthylamide wild-type, pH 8.0, 37°C Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00015
-
L-tyrosyl-L-phenylalanine hydroxamic acid wild-type, pH 8.0, 37°C Homo sapiens
0.00092
-
L-tyrosyl-L-phenylalanine hydroxamic acid mutant W300F, pH 8.0, 37°C Homo sapiens
0.00427
-
L-tyrosyl-L-phenylalanine hydroxamic acid mutant W300L, pH 8.0, 37°C Homo sapiens
0.0105
-
L-tyrosyl-glycine hydroxamic acid wild-type, pH 8.0, 37°C Homo sapiens
0.06162
-
L-tyrosyl-glycine hydroxamic acid mutant W300F, pH 8.0, 37°C Homo sapiens
0.351
-
L-tyrosyl-glycine hydroxamic acid mutant W300L, pH 8.0, 37°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.053
-
Ala-Ala-2-naphthylamide mutant W300L, pH 8.0, 37°C Homo sapiens
0.24
-
Ala-Ala-2-naphthylamide mutant W300F, pH 8.0, 37°C Homo sapiens
1.17
-
Ala-Ala-2-naphthylamide wild-type, pH 8.0, 37°C Homo sapiens
24.6
-
Arg-Arg-2-naphthylamide mutant W300L, pH 8.0, 37°C Homo sapiens
775.4
-
Arg-Arg-2-naphthylamide mutant W300F, pH 8.0, 37°C Homo sapiens
1879
-
Arg-Arg-2-naphthylamide wild-type, pH 8.0, 37°C Homo sapiens