Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
W300F | mutant with slight increase in activity compared to wild-type enzyme | Homo sapiens |
W300L | mutant with higher activity compared to wild-type enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-tyrosyl-glycine hydroxamic acid | - |
Homo sapiens | |
L-tyrosyl-L-phenylalanine hydroxamic acid | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0116 | - |
Arg-Arg-2-naphthylamide | wild-type, pH 8.0, 37°C | Homo sapiens | |
0.0138 | - |
Arg-Arg-2-naphthylamide | mutant W300F, pH 8.0, 37°C | Homo sapiens | |
0.057 | - |
Arg-Arg-2-naphthylamide | mutant W300L, pH 8.0, 37°C | Homo sapiens | |
0.299 | - |
Ala-Ala-2-naphthylamide | wild-type, pH 8.0, 37°C | Homo sapiens | |
0.355 | - |
Ala-Ala-2-naphthylamide | mutant W300F, pH 8.0, 37°C | Homo sapiens | |
0.441 | - |
Ala-Ala-2-naphthylamide | mutant W300L, pH 8.0, 37°C | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
82000 | - |
Western blot | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9NY33 | - |
- |
Purification (Comment) | Organism |
---|---|
about 28% yield after gel filtration and FPLC for wild-type enzyme | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.43 | - |
mutant W300L, pH 8.0, 37°C | Homo sapiens |
24.9 | - |
mutant W300F, pH 8.0, 37°C | Homo sapiens |
45.6 | - |
wild-type, pH 8.0, 37°C | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ala-Ala-2-naphthylamide + H2O | assay at 37°C, pH 8.0 | Homo sapiens | Ala-Ala + 2-naphtylamine | - |
? | |
Ala-Phe-2-naphthylamide + H2O | assay at 37°C, pH 8.0 | Homo sapiens | Ala-Phe + 2-naphtylamine | - |
? | |
Arg-Arg-2-naphthylamide + H2O | assay at 37°C, pH 8.0 | Homo sapiens | Arg-Arg + 2-naphthylamine | - |
? | |
Gly-Phe-2-naphthylamide + H2O | assay at 37°C, pH 8.0 | Homo sapiens | Gly-Phe + 2-naphthylamine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
dipeptidyl peptidase III | - |
Homo sapiens |
DPP III | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
50% enzyme inactivation at 50°C for wild-type enzyme and mutant W300F and mutant W300L | Homo sapiens |
55 | - |
wild-type enzyme and mutant W300F and mutant W300L completely inactivated | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
Ala-Ala-2-naphthylamide | mutant W300L, pH 8.0, 37°C | Homo sapiens | |
0.085 | - |
Ala-Ala-2-naphthylamide | mutant W300F, pH 8.0, 37°C | Homo sapiens | |
0.35 | - |
Ala-Ala-2-naphthylamide | wild-type, pH 8.0, 37°C | Homo sapiens | |
1.4 | - |
Arg-Arg-2-naphthylamide | mutant W300L, pH 8.0, 37°C | Homo sapiens | |
10.7 | - |
Arg-Arg-2-naphthylamide | mutant W300F, pH 8.0, 37°C | Homo sapiens | |
21.8 | - |
Arg-Arg-2-naphthylamide | wild-type, pH 8.0, 37°C | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00015 | - |
L-tyrosyl-L-phenylalanine hydroxamic acid | wild-type, pH 8.0, 37°C | Homo sapiens | |
0.00092 | - |
L-tyrosyl-L-phenylalanine hydroxamic acid | mutant W300F, pH 8.0, 37°C | Homo sapiens | |
0.00427 | - |
L-tyrosyl-L-phenylalanine hydroxamic acid | mutant W300L, pH 8.0, 37°C | Homo sapiens | |
0.0105 | - |
L-tyrosyl-glycine hydroxamic acid | wild-type, pH 8.0, 37°C | Homo sapiens | |
0.06162 | - |
L-tyrosyl-glycine hydroxamic acid | mutant W300F, pH 8.0, 37°C | Homo sapiens | |
0.351 | - |
L-tyrosyl-glycine hydroxamic acid | mutant W300L, pH 8.0, 37°C | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.053 | - |
Ala-Ala-2-naphthylamide | mutant W300L, pH 8.0, 37°C | Homo sapiens | |
0.24 | - |
Ala-Ala-2-naphthylamide | mutant W300F, pH 8.0, 37°C | Homo sapiens | |
1.17 | - |
Ala-Ala-2-naphthylamide | wild-type, pH 8.0, 37°C | Homo sapiens | |
24.6 | - |
Arg-Arg-2-naphthylamide | mutant W300L, pH 8.0, 37°C | Homo sapiens | |
775.4 | - |
Arg-Arg-2-naphthylamide | mutant W300F, pH 8.0, 37°C | Homo sapiens | |
1879 | - |
Arg-Arg-2-naphthylamide | wild-type, pH 8.0, 37°C | Homo sapiens |