Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
C113S | mutation of the cysteine residue | Saccharomyces cerevisiae |
C130S | mutant, only existence of dimeric form | Saccharomyces cerevisiae |
C518S | mutation of the cysteine residue | Saccharomyces cerevisiae |
C626S | mutation of the cysteine residue | Saccharomyces cerevisiae |
C639S | mutant, resistance against p-hydroxy-mercuribenzoate | Saccharomyces cerevisiae |
additional information | DPPIII6His, construct with hexahistidine | Saccharomyces cerevisiae |
General Stability | Organism |
---|---|
4,4'-dithiodipyridine, inactivation of wild-type dipeptidyl peptidase III after incubation at pH 8.0 at 25°C, after 5 min incubation loss of 30% enzyme activity, after 15 min incubation loss of 62% enzyme activity | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4,4'-dithiodipyridine | inactivation of wild-type dipeptidyl peptidase III after incubation at pH 8.0 at 25°C, after 5 min incubation loss of 30% enzyme activity, after 15 min incubation loss of 62% enzyme activity | Saccharomyces cerevisiae | |
Zn2+ | 10 micromol, strong inhibition | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00889 | - |
Arg-Arg-2-naphthylamide | mutant C130S, pH 8.0, 37°C | Saccharomyces cerevisiae | |
0.01041 | - |
Arg-Arg-2-naphthylamide | wild-type, pH 8.0, 37°C | Saccharomyces cerevisiae | |
0.01097 | - |
Arg-Arg-2-naphthylamide | mutant C639S, pH 8.0, 37°C | Saccharomyces cerevisiae | |
0.01191 | - |
Arg-Arg-2-naphthylamide | mutant C518S, pH 8.0, 37°C | Saccharomyces cerevisiae | |
0.01518 | - |
Arg-Arg-2-naphthylamide | wild type, with His-tag, pH 8.0, 37°C | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 10-100 micromol, potent activation | Saccharomyces cerevisiae | |
Zn2+ | 10 micromol, strong inhibition | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
82000 | - |
SDS-PAGE | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | Q08225 | - |
- |
Purification (Comment) | Organism |
---|---|
gel filtration and FPLC on Mono Q column | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ala-Ala-2-naphthylamide + H2O | - |
Saccharomyces cerevisiae | Ala-Ala + 2-naphthylamine | - |
? | |
Ala-Phe-2-naphthylamide + H2O | - |
Saccharomyces cerevisiae | Ala-Phe + 2-naphthylamine | - |
? | |
Arg-Arg-2-naphthylamide + H2O | assay at pH 8.0, 37°C | Saccharomyces cerevisiae | Arg-Arg + 2-naphthylamine | - |
? | |
His-Ser-2-naphthylamide + H2O | - |
Saccharomyces cerevisiae | His-Ser + 2-naphthylamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Saccharomyces cerevisiae |
monomer | - |
Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
dipeptidyl peptidase III | - |
Saccharomyces cerevisiae |
DPP III | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
Arg-Arg-2-naphthylamide | mutant C130S, pH 8.0, 37°C | Saccharomyces cerevisiae | |
0.131 | - |
Arg-Arg-2-naphthylamide | mutant C518S, pH 8.0, 37°C | Saccharomyces cerevisiae | |
0.249 | - |
Arg-Arg-2-naphthylamide | wild-type, with His-tag, pH 8.0, 37°C | Saccharomyces cerevisiae | |
0.256 | - |
Arg-Arg-2-naphthylamide | mutant C639S, pH 8.0, 37°C | Saccharomyces cerevisiae | |
0.334 | - |
Arg-Arg-2-naphthylamide | wild-type, pH 8.0, 37°C | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Saccharomyces cerevisiae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.21 | - |
Arg-Arg-2-naphthylamide | mutant C130S, pH 8.0, 37°C | Saccharomyces cerevisiae | |
10.86 | - |
Arg-Arg-2-naphthylamide | mutant C518S, pH 8.0, 37°C | Saccharomyces cerevisiae | |
16.74 | - |
Arg-Arg-2-naphthylamide | wild type, with His-tag, pH 8.0, 37°C | Saccharomyces cerevisiae | |
23.88 | - |
Arg-Arg-2-naphthylamide | mutant C639S, pH 8.0, 37°C | Saccharomyces cerevisiae | |
32.58 | - |
Arg-Arg-2-naphthylamide | wild-type, pH 8.0, 37°C | Saccharomyces cerevisiae |