Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dimethyl sulfoxide | the activity progressively increases with increase in dimethyl sulfoxide concentration up to 5% (v/v) | Capra hircus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3,3'-dithiobis(6-nitrobenzoic acid) | 13% residual activity at 2.5 mM, beta-mercaptoethanol and dithiothreitlol completely restores enzyme activity | Capra hircus | |
cysteine | - |
Capra hircus | |
dimethyl sulfoxide | inhibitory effect with more than 5% (v/v) dimethyl sulfoxide | Capra hircus | |
dithioerythreitol | - |
Capra hircus | |
dithiothreitol | - |
Capra hircus | |
EDTA | 49.2% residual activity at 1 mM | Capra hircus | |
ethanol | the enzyme loses about half of its activity at 5% (v/v) ethanol | Capra hircus | |
glutathione | - |
Capra hircus | |
Gly-L-Glu | 36% inhibition at 1 mM | Capra hircus | |
Gly-L-Phe | 46% inhibition at 1 mM | Capra hircus | |
Gly-L-Phe-L-Leu | non-competitive inhibitor, 82% inhibition at 1 mM | Capra hircus | |
Hg2+ | 11.1% residual activity at 0.1 mM | Capra hircus | |
K+ | 82.0% residual activity at 0.1 mM | Capra hircus | |
L-Ala-L-Ala-L-Ala | 4% inhibition at 1 mM | Capra hircus | |
L-Ala-L-Ala-L-Ala-L-Ala | 58% inhibition at 0.5 mM | Capra hircus | |
L-Arg-L-Phe | 56% inhibition at 1 mM | Capra hircus | |
L-Arg-L-Phe-L-Ala | competitive inhibitor, 99% inhibition at 1 mM | Capra hircus | |
L-Arg-L-Trp | 46% inhibition at 1 mM | Capra hircus | |
L-Arg-L-Val | 49% inhibition at 1 mM | Capra hircus | |
L-Asp-L-Ala | 25% inhibition at 1 mM | Capra hircus | |
L-His-L-Leu | 76% inhibition at 1 mM | Capra hircus | |
L-His-L-Lys | 41% inhibition at 1 mM | Capra hircus | |
L-His-L-Phe | 46% inhibition at 1 mM | Capra hircus | |
L-His-L-Pro | 16% inhibition at 1 mM | Capra hircus | |
L-Ile-L-His | 34% inhibition at 1 mM | Capra hircus | |
L-Leu-L-Arg | complete inhibition at 0.5 mM | Capra hircus | |
L-Leu-L-Trp | competitive inhibitor, 90% inhibition at 1 mM | Capra hircus | |
L-Leu-L-Trp-L-Met | competitive inhibitor, 92% inhibition at 1 mM | Capra hircus | |
L-Leu-L-Trp-L-Met-L-Arg-L-Phe-L-Ala | competitive inhibitor, complete inhibition at 1 mM | Capra hircus | |
L-Lys-L-Ala | 23% inhibition at 1 mM | Capra hircus | |
L-Met-L-Arg | 99% inhibition at 1 mM | Capra hircus | |
L-Phe-L-Ala | 41% inhibition at 1 mM | Capra hircus | |
L-Phe-L-Arg | 75% inhibition at 1 mM | Capra hircus | |
L-Phe-L-Leu | 58% inhibition at 1 mM | Capra hircus | |
L-Phe-L-Met | 85% inhibition at 1 mM | Capra hircus | |
L-Pro-L-Arg-OH | 54% inhibition at 1 mM | Capra hircus | |
L-Pro-L-Glu | 34% inhibition at 1 mM | Capra hircus | |
L-Pro-L-Phe | 56% inhibition at 1 mM | Capra hircus | |
L-Ser-L-Met | 32% inhibition at 1 mM | Capra hircus | |
L-Trp-L-Met | competitive inhibitor, 94% inhibition at 1 mM | Capra hircus | |
L-Trp-L-Met-L-Asp-L-Phe | 82% inhibition at 1 mM | Capra hircus | |
L-tryptophyl-L-methionyl-L-alpha-aspartyl-L-phenylalaninamide | competitive inhibition | Capra hircus | |
L-Tyr-Gly | 31% inhibition at 1 mM | Capra hircus | |
L-Tyr-Gly-Gly-L-Phe | 53% inhibition at 1 mM | Capra hircus | |
L-Tyr-L-Ile | 72% inhibition at 1 mM | Capra hircus | |
L-Val-L-Tyr | competitive inhibitor, 84% inhibition at 1 mM | Capra hircus | |
Leu-enkephalin | i.e. L-Tyr-Gly-Gly-L-Phe-L-Leu, 92% inhibition at 1 mM, competitive inhibitor | Capra hircus | |
Li+ | 73.0% residual activity at 0.1 mM | Capra hircus | |
Met-enkephalin | i.e. L-Tyr-Gly-Gly-L-Phe-L-Met, 61% inhibition at 0.5 mM, competitive inhibitor | Capra hircus | |
additional information | not inhibited by beta-mercaptoethanol | Capra hircus | |
Ni2+ | 12.4% residual activity at 0.1 mM | Capra hircus | |
thioglycolic acid | - |
Capra hircus | |
Urea | sensitive to very low concentrations of urea | Capra hircus | |
Zn2+ | 50.3% residual activity at 1 mM | Capra hircus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 174.4% relative activity at 0.1 mM | Capra hircus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Capra hircus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Capra hircus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Capra hircus | - |
Storage Stability | Organism |
---|---|
-20°C, in 50 mM Tris-HCl, pH 7.5, 1 and 6 months, 38.15% and 99.75% loss of activity | Capra hircus |
-20°C, in 50 mM Tris-HCl, pH 7.5, in the presence of 10% (v/v) glycerol, 1 and 6 months, 26.5% and 92% loss of activity | Capra hircus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Arg-L-Arg-4-methoxy-2-naphthylamide + H2O | - |
Capra hircus | L-Arg-L-Arg + 4-methoxy-2-naphthylamine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
dipeptidyl peptidase III | - |
Capra hircus |
DPP-III | - |
Capra hircus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
L-Arg-L-Phe-L-Ala | in 50 mM Tris-HCl, pH 8.5, with 100 mM NaCl and 1 mM beta-mercaptoethanol, at 37°C | Capra hircus | |
0.007 | - |
L-Leu-L-Trp-L-Met-L-Arg-L-Phe-L-Ala | in 50 mM Tris-HCl, pH 8.5, with 100 mM NaCl and 1 mM beta-mercaptoethanol, at 37°C | Capra hircus | |
0.015 | - |
Gly-L-Phe-L-Leu | in 50 mM Tris-HCl, pH 8.5, with 100 mM NaCl and 1 mM beta-mercaptoethanol, at 37°C | Capra hircus | |
0.031 | - |
L-Leu-L-Trp-L-Met | in 50 mM Tris-HCl, pH 8.5, with 100 mM NaCl and 1 mM beta-mercaptoethanol, at 37°C | Capra hircus | |
0.039 | - |
L-Leu-L-Trp | in 50 mM Tris-HCl, pH 8.5, with 100 mM NaCl and 1 mM beta-mercaptoethanol, at 37°C | Capra hircus | |
0.055 | - |
L-tryptophyl-L-methionyl-L-alpha-aspartyl-L-phenylalaninamide | in 50 mM Tris-HCl, pH 8.5, with 100 mM NaCl and 1 mM beta-mercaptoethanol, at 37°C | Capra hircus | |
0.09 | - |
Leu-enkephalin | in 50 mM Tris-HCl, pH 8.5, with 100 mM NaCl and 1 mM beta-mercaptoethanol, at 37°C | Capra hircus | |
0.13 | - |
Met-enkephalin | in 50 mM Tris-HCl, pH 8.5, with 100 mM NaCl and 1 mM beta-mercaptoethanol, at 37°C | Capra hircus | |
0.25 | - |
L-Val-L-Tyr | in 50 mM Tris-HCl, pH 8.5, with 100 mM NaCl and 1 mM beta-mercaptoethanol, at 37°C | Capra hircus | |
0.38 | - |
L-Trp-L-Met | in 50 mM Tris-HCl, pH 8.5, with 100 mM NaCl and 1 mM beta-mercaptoethanol, at 37°C | Capra hircus |