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Literature summary for 3.4.14.2 extracted from

  • MacFarlane, S.; MacFarlane, G.T.
    Formation of a dipeptidyl arylamidase by Bacteroides splanchnicus NCTC 10825 with specificities towards glycylprolyl-x and valylalanine-x substrates (1997), J. Med. Microbiol., 46, 547-555.
    View publication on PubMed
Search for more literature for 3.4.14.2

Inhibitors

Inhibitors Comment Organism Structure
iodoacetate
-
 Odoribacter splanchnicus
phenylmethylsulfonyl fluoride 5 mM, about 80% inhibition Odoribacter splanchnicus
thimerosal
-
 Odoribacter splanchnicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
 2 * 80000, SDS-PAGE Odoribacter splanchnicus
160000
-
 gel filtration Odoribacter splanchnicus

Organism

Organism UniProt Comment Textmining
Odoribacter splanchnicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Odoribacter splanchnicus

Source Tissue

Source Tissue Comment Organism Textmining
additional information the enzyme is largely cell-associated during exponential growth in batch culture, but is progressively released by the bacteria before the cells enter stationary phase. The enzyme is completely cell-bound during growth in continuous culture Odoribacter splanchnicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
azocasein + H2O
-
 Odoribacter splanchnicus ?
-
 ?
Gly-Pro-2-naphthylamide + H2O
-
 Odoribacter splanchnicus Gly-Pro + 2-naphthylamine
-
 ?
Gly-Pro-4-nitroanilide + H2O
-
 Odoribacter splanchnicus Gly-Pro + 4-nitroaniline
-
 ?
Val-Ala-4-nitroanilide + H2O
-
 Odoribacter splanchnicus Val-Ala + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 80000, SDS-PAGE Odoribacter splanchnicus

Synonyms

Synonyms Comment Organism
GPA
-
 Odoribacter splanchnicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.2
-
 Odoribacter splanchnicus